Future Design/ 8+ HPP
We were thinking that if we could get one strain of E.coli that is auxotrophic to all 20 amino acids we could split the amino acids and then follow our designed protocol and solve a 20 HPP at one time.
However, we could not find any strains of E. coli that are auxotrophic for all 20 amino acids. We were able to find 3 strains auxotrophic for at least two amino acids. So, we thought that we could mate the E. coli cells and get a strain of E. coli auxotrophic for 8 amino acids.
As a result, we need to find and obtain:
|What We Need||Status|
|Y10 E. coli Strain|| Found at Yale University, but have not requested a copy Picture of Yale University E.coli database results: Media:Yale.png
|58-161 E.coli Strain||Found at Yale University, but have not requested a copy|
|WWU E. coli Strain||Found at Yale University is call E. coli strain 15 T-, but have not requested a copy|
|Protocol for mating E. coli|| Protocol Options
|Protocol for changing a haploid to a diploid||HrF cross will work for mating protocol to get haploid|
Mutated Enzymes Causing Auxotroph's for Amino Acids: Media:AminoAcidAuxotrophs.pptx
Sequences of Genes causing Auxtrophs used in strain DL39: Media: aminacidsequences.docx