JP Jan 28 16
72 nucleotide reads. What gene do they go to? How many of each are there?
How to count number of genes and compare across time points? Normalize to a housekeeping gene. OR standard procedure (look up).
- "How to normalize gene expression given you don't get the same number of reads in each sample"
How to know we have the right tissue?
- Blast overrepresented sequences of trimmed intestinal sequences.
Have asked for a list of the genes expressed in each organ from the previous paper. (Todd?)
- How can we use the data repository, can we query? SRP051827
- Two teams to work in pairs to figure out a way to take the text file and add a number to "protein of unknown function" / see if other genes are named more than once.