January 21, 2016
"The Burmese Pythod Genome Reveals the Molecular Basis for Extreme Adaptation in Snakes" (2013)
- Researchers clustered genes with similar trends in differential gene expression together.
- STEM is a source of gene identifiers and clusters.
- Volcano plots showed significant difference for no change in gene expression levels.
Questions to Consider:
- What are the names of the genes the researchers clustered?
- Investigate ncbi gene bank
- What started the cascade?
- If known transcription factors activate genes in the cascade, maybe we can find them early on.
- If a few genes don't change, is that biologically significant?
Analyze FastQC data analysis results downloaded from class on January 19, 2016
- A quality score above 40 is good.
- Sequence codes match the reagents provided in class document.
- All reads are 76bp.
- Very few "N's." A "N" indicates that RNAseq was unable to pick a base.
- Intestine has smooth GC curve line.
- The class was unable to determine what exactly "Sequence Duplication Levels" showed us.
- Overall, our data is of good quality.
Moving forward, we need to:
- Trim off the first 4 bases.
- Discard reads with a score less than 15.
- Name all the reads.
- Sort all the reads.
- Exhaust publicly avaible data sets.
INCLUDE SCREENSHOTS OF DATA WHEN YOU FIGURE OUT HOW TO UPLOAD PICTURES
- Castoe, Todd A., et al. “The Burmese python genome reveals the molecular basis for extreme adaptation in snakes.” Pnas.org. (2013)