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Las/Rhl cell signaling system

Responsible: Robert Cool, Alicia Allen, and Erin Feeney

Las System

Signal Molecule: An AHL called PAI-1 (N-3-oxododecanoyl-l-hsl)(3-oxo-C12-hsl)

Bacterial species: Pseudomonas aeruginosa gram(-) possibly E.coli (see article 3)

Receiver protein: LasR

Effect of binding: TXN activation of virulence genes, lasA, lasB, apr, toxR

Synthase: LasI enzyme

Target Genes: lasI, lasA, lasB, apr, toxR

Regulation: unknown


"Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes"

JP Pearson, EC Pesci and BH Iglewski [1]

"Regulation of Pseudomonas Quinolone Signal Synthesis in Pseudomonas aeruginosa"

Dana S. Wade, M. Worth Calfee, Edson R. Rocha, Elizabeth A. Ling, Elana Engstrom, James P. Coleman, and Everett C. Pesci 2

Posttranscriptional Control of Quorum-Sensing-Dependent Virulence Genes by DksA in Pseudomonas aeruginosa

Florence Jude,Thilo Köhler,Pavel Branny,Karl Perron,Matthias P. Mayer,Rachel Comte, and Christian van Delden 3

Pending: [2]

Rhl System

Signal Molecule: An AHL called PAI-2, Plasminogen activator inhibitor-2, N-butanoyl-homoserine lactone (C4HSL)

Bacterial species: Pseudomonas aeruginosa, gram(-)

Receiver Protein: Rhl R

Effect of Binding: activation of Rhamnosyl Transferase, then making RL (rhamnolipid)

Synthase: RhlA and RhlB

Target Genes: pqsABCDE and phnAB

Regulation: unknown


background information on Las and Rhl

Las rhl.gif

Parts Needed:

LasR + pro/term

RhlR + pro/term

RhlI + pro/term

pqsABCDE + pro/term


pqsH + pro/term