LuxR/cI Test and Outcome
luxR/CI hybrid promoter is constitutively off. Ligate with GFP on the 3’ end. If luxR is present first then add AI-1, the bacteria should glow green. Then add CI, the bacteria should not glow.
Some promoter→LuxR+t+t+pLuxR/cI→GFP does not glows
Some promoter→LuxR+t+t+pLuxR/cI→GFP glows
Some promoter→LuxR+cI+t+t+pLuxR/cI→GFP does not glow
|LuxR||cI||AI-1 + GFP||AI-1 - GFP|