NHE Feb 2 Notes

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Nick Elder

Group 2 intestines

BLAST Results:

  • most were mitochondrial

We can download the sequences from the 2015 paper, but not the list of genes 2013 Paper has list of genes with gene ontology

We are using DESeq standard to normalize(?) data

  • used to normalize expression
    • normalizes for the length of the gene per million reads
    • FPKM values

Used R and DESeq data to generate heat maps of differentially expressed genes in both liver and intestine.

Also tired to separate fed and fasted within the liver and intestine groups. (Modified R code)

  • noticed that one of the fed (animal 4?) was being grouped as more similar to the fasted animals than the other fed.