pMnt/Lac is constitutively on but is repressed by the LacI protein. The promoter is also repressed by the Mnt protein. The addition of IPTG should repress the LacI repressor and glow in the absence of the Mnt protein.
Plan to Test the promoter
To Test repression using LacI
Some promoter→LacI+t+t+pLac/Mnt→GFP does not glow
To Test repression using Mnt protein
Some promoter→Mnt+t+t+pLac/Mnt→GFP does not glow
To Test pLac being on with addition of IPTG
Some promoter→LacI+t+t+pLac/Mnt→GFP glows
To Test the repression of pLac
Some promoter→LacI+Mnt+t+t+pLac/Mnt→GFP does not glow
|LacI||Mnt||IPTG + GFP||IPTG - GFP|
The plasmid + vector was then transformed onto an amp plate.