Promoter Strength Metric

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P=Fss(u) (1+u/m)+ fssD((2u/m)+(D/m)+1)

u=growth rate m=maturation constant for oxygen-dependent fluorophore activation of GFP
D=first-order rate constant for protease-mediated degredation
fss=exponential-phase steady state concentration of GFP
P=rate of promoter driven production of GFP

Main Idea:

Flouresence/absorbance per hour is measured by the rate of production, the amount of GFP in the cell, and the time GFP stays in the cell. The equation is one of the measures used to characterize the mutant promoters' strength.