Repeating Eckdahl's 20-clone selection
We need to replicate Dr. Eckdahl’s 20-clone experiment twice on both campuses and mathematically analyze the data. Continue adding arabinose to induce the chaperones.
We need to grow the surviving clones on individual plates: no antibiotic, amp, amp+chlor. This will enable us to determine whether both plasmids (CDM+amp, chaperone+chlor) are still present and being transcribed.
Depending on the survivorship of the clones in the previous step, set up plates to run head-to-head competition between two surviving clones, and determine which strain is most competent. This will confirm the results of the 20-clone experiment, in a more controlled environment than a plate with all 20 clones.
Protocol created on 05/21/14: Eckdahl-replication-protocol
Photos from Davidson's first replication (four plates) (Updated May 31, 2014)
Template for results: File:Replication Results Template.xlsx Upload a file for each campus with the results displayed in the table. There is a tab in the file for each trial.