Claudia's Assignment

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Introduction

From the background information on our species, Halomicrobium mukohataei, we know that our organism is not only a halophile, or salt-loving, but also that the organism in fact requires a high salinity environment to survive. This trait is unique and compelling because of the harsh environment and the many pumps and mechanisms that must be present for an living organism to survive. However, our organism is not the only one to have such adaptations. There are a number of halophile species as well as a number of salt-tolerant organisms, including bacteria and yeasts.

Cation Pumps

The following is a list of the cation pumps and cation-influenced structures encoded in our organism's genome:

Na+/proline symporter 644032116
A BLAST (megablast) of the gene encoding this ion pump produced the following results:
Picture 5.png

Kef-type K+ ransport system, predicted NAD-binding component 644032129
A BLAST using megablast produced no results other than our own species.
A BLAST (blastn) of the gene encoding this ion pump produced the following results:
Picture 6.png

Ca2+/Na+ antiporter 644032240
A BLAST using megablast produced no results other than our own species.
A BLAST using blastn produced the following results:
Picture 7.png

Mn2+/Fe2_ transporter, NRAMP family 644032307
A BLAST using megablast produced the following results:
Picture 8.png

H(+)-transporting ATP synthase, vacuolar type, subunit D ( EC:3.6.3.14 ) 644032373
A BLAST using megablast produced the following results:
Picture 10.png
A BLAST using blastn produced the following results:
Picture 11.png

archaeal/vacuolar-type H+-ATPase subunit B ( EC:3.6.3.14 ) 644032378
A BLAST using megablast produced the following results:
Picture 12.png
A BLAST using blastn produced the following results:
Picture 13.png

archaeal/vacuolar-type H+-ATPase subunit F ( EC:3.6.3.14 ) 644032380
A BLAST using megablast produced no results other than our own species.
A BLAST using blastn produced the following results:
Picture 14.png

archaeal/vacuolar-type H+-ATPase subunit E ( EC:3.6.3.14 ) 644032382
A BLAST using megablast produced no results other than our own species.
A BLAST using blastn produced the following results:
Picture 15.png

archaeal/vacuolar-type H+-ATPase subunit I ( EC:3.6.3.14 ) 644032384
A BLAST using megablast produced the following results:
Picture 16.png
A BLAST using blastn produced the following results:
Picture 17.png

K+ transport system, NAD-binding component 644032472
A BLAST using megablast produced no results other than our own species.
A BLAST using blast n produced the following results:
Picture 18.png

NAD+ synthetase ( EC:6.3.5.1 ) 644032488
A BLAST using megablast produced no results other than our own species.
A BLAST using blastn produced the following results:
Picture 19.png

NH(3)-dependent NAD(+) synthetase (EC 6.3.1.5) (IMGterm) 644032494
A BLAST using megablast produced the following results:
Picture 20.png
Picture 22.png
Picture 21.png
A BLAST using blastn produced the following results:
Picture 24.png