RRNA operon 2009

From GcatWiki
Revision as of 12:55, 1 September 2009 by Mereilly (talk | contribs)

Jump to: navigation, search

Ribosomal RNA (rRNA) is the central component of the ribosome, the protein manufacturing machinery of all living cells. The function of the rRNA is to provide a mechanism for decoding mRNA into amino acids and to interact with the tRNAs during translation by providing peptidyl transferase activity.The tRNA then brings the necessary amino acids corresponding to the appropriate mRNA codon. Archaea contains either a single rDNA operon or multiple copies of the operon.

Archea usually contains three kinds of rRNA: 16S, 5S and 23S. 5S and 23S are part of the large ribosomal subunit, whereas 16S is part of the small robosomal unit and is responsible for recognizing the Shine-Delgarno sequences on most mRNAs. 16S is one of the most highly conserved sequences in any genome. 23S has peptidyl transferase activity and assists in binding adjacent amino acids during translation of an mRNA. Chloramphenicol acts by binding to the 23S rRNA so studying this gene in particular might explain why our species is chloramphenicol resistant.

Strangely enough, All the suggested 5S rRNAs in our genome were identical, as shown in the BLAST2 comparison here: Mr11.png

5s rRNA (445..583(+); 1766895..1767033(-); 1962..2100(+)), all three consisted of 139 identical base pairs.


16s rRNA (1770405..1771883(-); 1479bp): Top three species with similar 16s rRNA sequences




16s rRNA (3107428..3108907(+); 1480bp): Top three species with similar 16s rRNA sequences




16s rRNA (218960..219414(+); 455bp): Top three species with similar 16s rRNA sequences