Nanodrop DNA for Bio113

From GcatWiki
Jump to: navigation, search

Nanodrop DNA

  1. The Nanodrop will be blanked using the elution buffer from your mini prep.
  2. Pipet 2 µL of your mini prep onto the small open stop on the metal circle.
  3. Carefully lower the arm and click on the measure button.
  4. Record these values for each of your samples:
  • absorbance value of your DNA at 260 nm
  • concentration of your DNA in ng/µL
  • 260/280 ratio value