Difference between revisions of "Pouring an Agarose Gel"

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0.35 grams of Agarose
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///Pouring an Agarose Gel///
35 mL of TAE Buffer
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• Add both to an Erlenmeyer Flask and microwave for 1 minute
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#Go to http://www.bio.davidson.edu/courses/Molbio/Protocols/molwt.html to determine the percentage of agarose and calculate amount of agarose for 35 ml.
Swirl and microwave for 10 seconds
+
 
Let flask cool enough to touch, add 5 µL of Ethidium Bromide (WEAR GLOVES)
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# Weigh out agarose in 125 ml flask (eg. 0.35 grams agarose for 1% gel).
Swirl to mix and pour into gel tray
+
# Add 35 mL of TAE buffer
 +
# Microwave for 1 minute
 +
# Swirl and microwave for 10 seconds
 +
# Let flask cool enough to touch, add 5 µL of 10 mg/ml Ethidium Bromide (WEAR GLOVES)
 +
# Swirl to mix and pour into gel tray with comb

Revision as of 11:12, 30 June 2011

///Pouring an Agarose Gel///

  1. Go to http://www.bio.davidson.edu/courses/Molbio/Protocols/molwt.html to determine the percentage of agarose and calculate amount of agarose for 35 ml.
  1. Weigh out agarose in 125 ml flask (eg. 0.35 grams agarose for 1% gel).
  2. Add 35 mL of TAE buffer
  3. Microwave for 1 minute
  4. Swirl and microwave for 10 seconds
  5. Let flask cool enough to touch, add 5 µL of 10 mg/ml Ethidium Bromide (WEAR GLOVES)
  6. Swirl to mix and pour into gel tray with comb