Difference between revisions of "Pouring an Agarose Gel"

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• 0.35 grams of Agarose
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'''Pouring an Agarose Gel'''
35 mL of TAE Buffer
+
 
Add both to an Erlenmeyer Flask and microwave for 1 minute
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# Add 35 mL of 1X TAE buffer to a 125 ml flask
Swirl and microwave for 10 seconds
+
# Add agarose (0.35 grams agarose for 1% gel).
• Let flask cool enough to touch, add 5 µL of Ethidium Bromide (WEAR GLOVES)
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# Microwave for 50 seconds
Swirl to mix and pour into gel tray
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# Swirl and microwave for 5 seconds - repeat 3 times
 +
# Place flask in 55 C water bath to cool
 +
# Add 5 µL of 10 mg/ml Ethidium Bromide (CAUTION: WEAR GLOVES)
 +
# Swirl to mix and pour into gel tray with comb

Latest revision as of 17:03, 15 February 2017

Pouring an Agarose Gel

  1. Add 35 mL of 1X TAE buffer to a 125 ml flask
  2. Add agarose (0.35 grams agarose for 1% gel).
  3. Microwave for 50 seconds
  4. Swirl and microwave for 5 seconds - repeat 3 times
  5. Place flask in 55 C water bath to cool
  6. Add 5 µL of 10 mg/ml Ethidium Bromide (CAUTION: WEAR GLOVES)
  7. Swirl to mix and pour into gel tray with comb