Pouring an Agarose Gel

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//Pouring an Agarose Gel//

  1. Go to http://www.bio.davidson.edu/courses/Molbio/Protocols/molwt.html to determine the percentage of agarose and calculate amount of agarose for 35 ml.
  1. Weigh out agarose in 125 ml flask (eg. 0.35 grams agarose for 1% gel).
  2. Add 35 mL of TAE buffer
  3. Microwave for 1 minute
  4. Swirl and microwave for 10 seconds
  5. Let flask cool enough to touch, add 5 µL of 10 mg/ml Ethidium Bromide (WEAR GLOVES)
  6. Swirl to mix and pour into gel tray with comb