https://gcat.davidson.edu/GcatWiki/api.php?action=feedcontributions&feedformat=atom&user=XiZhu
GcatWiki - User contributions [en]
2026-05-05T15:43:09Z
User contributions
MediaWiki 1.28.2
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=6007
LsrA/cI
2008-07-28T15:27:37Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': <br />
<br />
'''pBAD+RBS+lsrK+RBS+lsrR+TT'''<br />
<br />
'''pLsrA/cI+RBS+LuxI+RBS+Mnt+TT'''<br />
<br />
'''I13453'''+'''B0034'''+'''K091002'''+'''B0034'''+'''K091001'''+'''B0015'''<br />
<br />
'''K091106'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| B0034 ||RBS ||R/X; S/P||S/P || 12+2079||K091001||lsrR ||R/X;S/P ||X/P ||954 ||K091138 ||972<br />
|-<br />
| 2|| B0034 ||RBS ||R/X; S/P||S/P || 12+2079||K091002||lsrK ||R/X;S/P ||X/P ||1593 ||K091158 ||1611<br />
|-<br />
| 3||I13453||pBAD ||R/X;S/P ||S/P || 120+2079||TBD||RBS+lsrK ||R/X; S/P ||X/P ||1611 ||K091159 || 1866<br />
|-<br />
| 4||B0015||TT||R/X; S/P ||R/X ||129+2079 ||K091138 ||RBS+lsrR ||R/X; S/P ||R/S || 972||K091139 ||1109<br />
|-<br />
| 5||K091139||RBS+lsrR+TT||R/X; S/P ||R/X ||1109+2079 ||TBD ||pBAD+RBA+lsrK ||R/X; S/P ||R/S ||1866 ||TBD ||<br />
|-<br />
| 6|| K091106 ||pLsrA/cI ||R/X; S/P ||S/P || 141+2079||C0261||RBS+luxI ||R/X; S/P ||X/P ||661 ||K091140||810<br />
|-<br />
| 7||B0015 ||TT ||R/X; S/P ||R/X ||129+2079 ||I13025 ||RBS+Mnt ||R/X;S/P ||R/S ||331 ||K091141 ||468<br />
|-<br />
| 8||K091141||RBS+Mnt+TT ||R/X; S/P ||R/X || 468+2079||K091140 ||pLsrA/cI+RBS+luxI ||R/X; S/P ||R/S ||810 || K091142||1286<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="110"|Description<br />
!width="60"|Source<br />
!width="100"|location('07)<br />
!width="35"|Vector<br />
!width="40"|Responsible<br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091001 K091001] ||lsrR ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091002 K091002] ||lsrK ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091106 K091106] ||plsrA/cI ||MW ||N/A ||N/A || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=6006
LsrA/cI
2008-07-28T15:26:51Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': <br />
<br />
'''pBAD+RBS+lsrK+RBS+lsrR+TT'''<br />
<br />
'''pLsrA/cI+RBS+LuxI+RBS+Mnt+TT'''<br />
<br />
'''I13453'''+'''B0034'''+'''K091002'''+'''B0034'''+'''K091001'''+'''B0015'''<br />
<br />
'''K091106'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| B0034 ||RBS ||R/X; S/P||S/P || 12+2079||K091001||lsrR ||R/X;S/P ||X/P ||954 ||K091138 ||972<br />
|-<br />
| 2|| B0034 ||RBS ||R/X; S/P||S/P || 12+2079||K091002||lsrK ||R/X;S/P ||X/P ||1593 ||K091158 ||1611<br />
|-<br />
| 3||I13453||pBAD ||R/X;S/P ||S/P || 120+2079||TBD||RBS+lsrK ||R/X; S/P ||X/P || ||K091159 || 1866<br />
|-<br />
| 4||B0015||TT||R/X; S/P ||R/X ||129+2079 ||K091138 ||RBS+lsrR ||R/X; S/P ||R/S || 972||K091139 ||1109<br />
|-<br />
| 5||K091139||RBS+lsrR+TT||R/X; S/P ||R/X ||1109+2079 ||TBD ||pBAD+RBA+lsrK ||R/X; S/P ||R/S || ||TBD ||<br />
|-<br />
| 6|| K091106 ||pLsrA/cI ||R/X; S/P ||S/P || 141+2079||C0261||RBS+luxI ||R/X; S/P ||X/P ||661 ||K091140||810<br />
|-<br />
| 7||B0015 ||TT ||R/X; S/P ||R/X ||129+2079 ||I13025 ||RBS+Mnt ||R/X;S/P ||R/S ||331 ||K091141 ||468<br />
|-<br />
| 8||K091141||RBS+Mnt+TT ||R/X; S/P ||R/X || 468+2079||K091140 ||pLsrA/cI+RBS+luxI ||R/X; S/P ||R/S ||810 || K091142||1286<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="110"|Description<br />
!width="60"|Source<br />
!width="100"|location('07)<br />
!width="35"|Vector<br />
!width="40"|Responsible<br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091001 K091001] ||lsrR ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091002 K091002] ||lsrK ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091106 K091106] ||plsrA/cI ||MW ||N/A ||N/A || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=Test_XOR_Hybrid_Promoters&diff=5959
Test XOR Hybrid Promoters
2008-07-18T15:27:52Z
<p>XiZhu: </p>
<hr />
<div>This are our ideas of how to test the four hybrid promoters for the XOR gate.<br />
<br />
<br />
[[Test pLuxR/cI]]<br />
<br />
<br />
<br />
[[Test pMnt/Lac]]<br />
<br />
<br />
[[Test pLsrA/cI]]<br />
<br />
<br />
<br />
[[Test pLasR/cI]]<br />
<br />
<br />
<br />
[[Test Mnt/Tet promoter]]<br />
<br />
<br />
<br />
'''Overlapping Parts And intermediates'''<br />
<br />
<br />
{| border="1" cellpadding="2"<br />
!width="30"|LuxR/cI<br />
!width="30"|Lac/Mnt<br />
!width="30"|LsrR/cI<br />
!width="30"|LasR/cI<br />
!width="30"|Mnt/Tet<br />
|-<br />
| RBS+cI(+TT) || || RBS+cI(+TT) || || <br />
|-<br />
| || RBS+Mnt(+TT) || || RBS+Mnt(+TT) || <br />
|-<br />
| || || || ||<br />
|-<br />
| || || || ||<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LasR/cI&diff=5953
LasR/cI
2008-07-17T15:58:25Z
<p>XiZhu: </p>
<hr />
<div>'''Goal:'''<br />
<br />
'''pBAD+RBS+lasR+TT'''(James DC)<br />
<br />
'''S0388?'''(I13453+B0034+C0079+B0015)<br />
<br />
'''pLasR/cI+RBS+LuxI+RBS+Mnt+TT'''<br />
<br />
'''K09110?'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| K091143 ||plasR/cI ||R/X; S/P||S/P || 164+2079||C0261||RBS+luxI ||R/X;S/P ||X/P ||661 ||TBD ||<br />
|-<br />
| 2|| B0015 ||TT ||R/X; S/P||R/X || 129+2079||I13025||RBS+Mnt ||R/X;S/P ||R/S ||331 ||TBD ||<br />
|-<br />
| 3||K091141||RBS+Mnt+TT ||R/X;S/P ||R/X ||468+2079 ||TBD||plasR/cI+RBS+luxI ||R/X; S/P ||R/S || ||TBD || <br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="110"|Description<br />
!width="60"|Source<br />
!width="100"|location('07)<br />
!width="35"|Vector<br />
!width="40"|Responsible<br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K09110? K09110?] ||plasR/cI ||MW ||N/A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LasR/cI&diff=5946
LasR/cI
2008-07-15T18:30:21Z
<p>XiZhu: </p>
<hr />
<div>'''Goal:'''<br />
<br />
'''pBAD+RBS+lasR+TT'''(James DC)<br />
<br />
'''S0388?'''(I13453+B0034+C0079+B0015)<br />
<br />
'''pLasR/cI+RBS+LuxI+RBS+Mnt+TT'''<br />
<br />
'''K09110?'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| K091143 ||plasR/cI ||R/X; S/P||S/P || 164+2079||C0261||RBS+luxI ||R/X;S/P ||X/P ||661 ||TBD ||<br />
|-<br />
| 2|| B0015 ||TT ||R/X; S/P||R/X || 129+2079||I13025||RBS+Mnt ||R/X;S/P ||R/S ||331 ||TBD ||<br />
|-<br />
| 3||TBD||RBS+Mnt+TT ||R/X;S/P ||R/X || ||TBD||plasR/cI+RBS+luxI ||R/X; S/P ||R/S || ||TBD || <br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="110"|Description<br />
!width="60"|Source<br />
!width="100"|location('07)<br />
!width="35"|Vector<br />
!width="40"|Responsible<br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K09110? K09110?] ||plasR/cI ||MW ||N/A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5945
LsrA/cI
2008-07-15T18:13:22Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': <br />
<br />
'''pBAD+RBS+lsrK+RBS+lsrR+TT'''<br />
<br />
'''pLsrA/cI+RBS+LuxI+RBS+Mnt+TT'''<br />
<br />
'''I13453'''+'''B0034'''+'''K091002'''+'''B0034'''+'''K091001'''+'''B0015'''<br />
<br />
'''K091106'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| B0034 ||RBS ||R/X; S/P||S/P || 12+2079||K091001||lsrR ||R/X;S/P ||X/P ||954 ||K091138 ||972<br />
|-<br />
| 2|| B0034 ||RBS ||R/X; S/P||S/P || 12+2079||K091002||lsrK ||R/X;S/P ||X/P ||1593 ||TBD ||<br />
|-<br />
| 3||I13453||pBAD ||R/X;S/P ||S/P || 120+2079||TBD||RBS+lsrK ||R/X; S/P ||X/P || ||TBD || <br />
|-<br />
| 4||B0015||TT||R/X; S/P ||R/X ||129+2079 ||K091138 ||RBS+lsrR ||R/X; S/P ||R/S || 972||K091139 ||1109<br />
|-<br />
| 5||K091139||RBS+lsrR+TT||R/X; S/P ||R/X ||1109+2079 ||TBD ||pBAD+RBA+lsrK ||R/X; S/P ||R/S || ||TBD ||<br />
|-<br />
| 6|| K091106 ||pLsrA/cI ||R/X; S/P ||S/P || 141+2079||C0261||RBS+luxI ||R/X; S/P ||X/P ||661 ||K091140||810<br />
|-<br />
| 7||B0015 ||TT ||R/X; S/P ||R/X ||129+2079 ||I13025 ||RBS+Mnt ||R/X;S/P ||R/S ||331 ||K091141 ||468<br />
|-<br />
| 8||K091141||RBS+Mnt+TT ||R/X; S/P ||R/X || 468+2079||K091140 ||pLsrA/cI+RBS+luxI ||R/X; S/P ||R/S ||810 || K091142||1286<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="110"|Description<br />
!width="60"|Source<br />
!width="100"|location('07)<br />
!width="35"|Vector<br />
!width="40"|Responsible<br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091001 K091001] ||lsrR ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091002 K091002] ||lsrK ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091106 K091106] ||plsrA/cI ||MW ||N/A ||N/A || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5944
LsrA/cI
2008-07-15T18:09:05Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': <br />
<br />
'''pBAD+RBS+lsrK+RBS+lsrR+TT'''<br />
<br />
'''pLsrA/cI+RBS+LuxI+RBS+Mnt+TT'''<br />
<br />
'''I13453'''+'''B0034'''+'''K091002'''+'''B0034'''+'''K091001'''+'''B0015'''<br />
<br />
'''K091106'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| B0034 ||RBS ||R/X; S/P||S/P || 12+2079||K091001||lsrR ||R/X;S/P ||X/P ||954 ||K091138 ||972<br />
|-<br />
| 2|| B0034 ||RBS ||R/X; S/P||S/P || 12+2079||K091002||lsrK ||R/X;S/P ||X/P ||1593 ||TBD ||<br />
|-<br />
| 3||I13453||pBAD ||R/X;S/P ||S/P || 120+2079||TBD||RBS+lsrK ||R/X; S/P ||X/P || ||TBD || <br />
|-<br />
| 4||B0015||TT||R/X; S/P ||R/X ||129+2079 ||TBD ||RBS+lsrR ||R/X; S/P ||R/S || ||K091139 ||1109<br />
|-<br />
| 5||K091139||RBS+lsrR+TT||R/X; S/P ||R/X ||1109+2079 ||TBD ||pBAD+RBA+lsrK ||R/X; S/P ||R/S || ||TBD ||<br />
|-<br />
| 6|| K091106 ||pLsrA/cI ||R/X; S/P ||S/P || 141+2079||C0261||RBS+luxI ||R/X; S/P ||X/P ||661 ||K091140||810<br />
|-<br />
| 7||B0015 ||TT ||R/X; S/P ||R/X ||129+2079 ||I13025 ||RBS+Mnt ||R/X;S/P ||R/S ||331 ||K091141 ||468<br />
|-<br />
| 8||K091141||RBS+Mnt+TT ||R/X; S/P ||R/X || 468||K091140 ||pLsrA/cI+RBS+luxI ||R/X; S/P ||R/S ||810 || TBD||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="110"|Description<br />
!width="60"|Source<br />
!width="100"|location('07)<br />
!width="35"|Vector<br />
!width="40"|Responsible<br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091001 K091001] ||lsrR ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091002 K091002] ||lsrK ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091106 K091106] ||plsrA/cI ||MW ||N/A ||N/A || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5943
LsrA/cI
2008-07-15T18:05:01Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': <br />
<br />
'''pBAD+RBS+lsrK+RBS+lsrR+TT'''<br />
<br />
'''pLsrA/cI+RBS+LuxI+RBS+Mnt+TT'''<br />
<br />
'''I13453'''+'''B0034'''+'''K091002'''+'''B0034'''+'''K091001'''+'''B0015'''<br />
<br />
'''K091106'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| B0034 ||RBS ||R/X; S/P||S/P || 12+2079||K091001||lsrR ||R/X;S/P ||X/P ||954 ||K091138 ||972<br />
|-<br />
| 2|| B0034 ||RBS ||R/X; S/P||S/P || 12+2079||K091002||lsrK ||R/X;S/P ||X/P ||1593 ||TBD ||<br />
|-<br />
| 3||I13453||pBAD ||R/X;S/P ||S/P || 120+2079||TBD||RBS+lsrK ||R/X; S/P ||X/P || ||TBD || <br />
|-<br />
| 4||B0015||TT||R/X; S/P ||R/X ||129+2079 ||TBD ||RBS+lsrR ||R/X; S/P ||R/S || ||K091139 ||1109<br />
|-<br />
| 5||TBD||RBS+lsrR+TT||R/X; S/P ||R/X || ||TBD ||pBAD+RBA+lsrK ||R/X; S/P ||R/S || ||TBD ||<br />
|-<br />
| 6|| K091106 ||pLsrA/cI ||R/X; S/P ||S/P || 141+2079||C0261||RBS+luxI ||R/X; S/P ||X/P ||661 ||K091140||810<br />
|-<br />
| 7||B0015 ||TT ||R/X; S/P ||R/X ||129+2079 ||I13025 ||RBS+Mnt ||R/X;S/P ||R/S ||331 ||TBD ||<br />
|-<br />
| 8||TBD ||RBS+Mnt+TT ||R/X; S/P ||R/X || ||TBD ||pLsrA/cI+RBS+luxI ||R/X; S/P ||R/S || || TBD||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="110"|Description<br />
!width="60"|Source<br />
!width="100"|location('07)<br />
!width="35"|Vector<br />
!width="40"|Responsible<br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091001 K091001] ||lsrR ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091002 K091002] ||lsrK ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091106 K091106] ||plsrA/cI ||MW ||N/A ||N/A || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5942
LsrA/cI
2008-07-15T18:01:04Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': <br />
<br />
'''pBAD+RBS+lsrK+RBS+lsrR+TT'''<br />
<br />
'''pLsrA/cI+RBS+LuxI+RBS+Mnt+TT'''<br />
<br />
'''I13453'''+'''B0034'''+'''K091002'''+'''B0034'''+'''K091001'''+'''B0015'''<br />
<br />
'''K091106'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| B0034 ||RBS ||R/X; S/P||S/P || 12+2079||K091001||lsrR ||R/X;S/P ||X/P ||954 ||K091138 ||972<br />
|-<br />
| 2|| B0034 ||RBS ||R/X; S/P||S/P || 12+2079||K091002||lsrK ||R/X;S/P ||X/P ||1593 ||TBD ||<br />
|-<br />
| 3||I13453||pBAD ||R/X;S/P ||S/P || 120+2079||TBD||RBS+lsrK ||R/X; S/P ||X/P || ||TBD || <br />
|-<br />
| 4||B0015||TT||R/X; S/P ||R/X ||129+2079 ||TBD ||RBS+lsrR ||R/X; S/P ||R/S || ||K091139 ||1109<br />
|-<br />
| 5||TBD||RBS+lsrR+TT||R/X; S/P ||R/X || ||TBD ||pBAD+RBA+lsrK ||R/X; S/P ||R/S || ||TBD ||<br />
|-<br />
| 6|| K091106 ||pLsrA/cI ||R/X; S/P ||S/P || 141+2079||C0261||RBS+luxI ||R/X; S/P ||X/P ||661 ||TBD||<br />
|-<br />
| 7||B0015 ||TT ||R/X; S/P ||R/X ||129+2079 ||I13025 ||RBS+Mnt ||R/X;S/P ||R/S ||331 ||TBD ||<br />
|-<br />
| 8||TBD ||RBS+Mnt+TT ||R/X; S/P ||R/X || ||TBD ||pLsrA/cI+RBS+luxI ||R/X; S/P ||R/S || || TBD||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="110"|Description<br />
!width="60"|Source<br />
!width="100"|location('07)<br />
!width="35"|Vector<br />
!width="40"|Responsible<br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091001 K091001] ||lsrR ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091002 K091002] ||lsrK ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091106 K091106] ||plsrA/cI ||MW ||N/A ||N/A || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5941
LsrA/cI
2008-07-15T17:57:54Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': <br />
<br />
'''pBAD+RBS+lsrK+RBS+lsrR+TT'''<br />
<br />
'''pLsrA/cI+RBS+LuxI+RBS+Mnt+TT'''<br />
<br />
'''I13453'''+'''B0034'''+'''K091002'''+'''B0034'''+'''K091001'''+'''B0015'''<br />
<br />
'''K091106'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| B0034 ||RBS ||R/X; S/P||S/P || 12+2079||K091001||lsrR ||R/X;S/P ||X/P ||954 ||K091138 ||972<br />
|-<br />
| 2|| B0034 ||RBS ||R/X; S/P||S/P || 12+2079||K091002||lsrK ||R/X;S/P ||X/P ||1593 ||TBD ||<br />
|-<br />
| 3||I13453||pBAD ||R/X;S/P ||S/P || 120+2079||TBD||RBS+lsrK ||R/X; S/P ||X/P || ||TBD || <br />
|-<br />
| 4||B0015||TT||R/X; S/P ||R/X ||129+2079 ||TBD ||RBS+lsrR ||R/X; S/P ||R/S || ||TBD ||<br />
|-<br />
| 5||TBD||RBS+lsrR+TT||R/X; S/P ||R/X || ||TBD ||pBAD+RBA+lsrK ||R/X; S/P ||R/S || ||TBD ||<br />
|-<br />
| 6|| K091106 ||pLsrA/cI ||R/X; S/P ||S/P || 141+2079||C0261||RBS+luxI ||R/X; S/P ||X/P ||661 ||TBD||<br />
|-<br />
| 7||B0015 ||TT ||R/X; S/P ||R/X ||129+2079 ||I13025 ||RBS+Mnt ||R/X;S/P ||R/S ||331 ||TBD ||<br />
|-<br />
| 8||TBD ||RBS+Mnt+TT ||R/X; S/P ||R/X || ||TBD ||pLsrA/cI+RBS+luxI ||R/X; S/P ||R/S || || TBD||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="110"|Description<br />
!width="60"|Source<br />
!width="100"|location('07)<br />
!width="35"|Vector<br />
!width="40"|Responsible<br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091001 K091001] ||lsrR ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091002 K091002] ||lsrK ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091106 K091106] ||plsrA/cI ||MW ||N/A ||N/A || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5933
LsrA/cI
2008-07-11T20:10:47Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': <br />
<br />
'''pBAD+RBS+lsrK+RBS+lsrR+TT'''<br />
<br />
'''pLsrA/cI+RBS+LuxI+RBS+Mnt+TT'''<br />
<br />
'''I13453'''+'''B0034'''+'''K091002'''+'''B0034'''+'''K091001'''+'''B0015'''<br />
<br />
'''K091106'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| B0034 ||RBS ||R/X; S/P||S/P || 12+2079||K091001||lsrR ||R/X;S/P ||X/P ||954 ||TBD ||<br />
|-<br />
| 2|| B0034 ||RBS ||R/X; S/P||S/P || 12+2079||K091002||lsrK ||R/X;S/P ||X/P ||1593 ||TBD ||<br />
|-<br />
| 3||I13453||pBAD ||R/X;S/P ||S/P || 120+2079||TBD||RBS+lsrK ||R/X; S/P ||X/P || ||TBD || <br />
|-<br />
| 4||B0015||TT||R/X; S/P ||R/X ||129+2079 ||TBD ||RBS+lsrR ||R/X; S/P ||R/S || ||TBD ||<br />
|-<br />
| 5||TBD||RBS+lsrR+TT||R/X; S/P ||R/X || ||TBD ||pBAD+RBA+lsrK ||R/X; S/P ||R/S || ||TBD ||<br />
|-<br />
| 6|| K091106 ||pLsrA/cI ||R/X; S/P ||S/P || 141+2079||C0261||RBS+luxI ||R/X; S/P ||X/P ||661 ||TBD||<br />
|-<br />
| 7||B0015 ||TT ||R/X; S/P ||R/X ||129+2079 ||I13025 ||RBS+Mnt ||R/X;S/P ||R/S ||331 ||TBD ||<br />
|-<br />
| 8||TBD ||RBS+Mnt+TT ||R/X; S/P ||R/X || ||TBD ||pLsrA/cI+RBS+luxI ||R/X; S/P ||R/S || || TBD||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="110"|Description<br />
!width="60"|Source<br />
!width="100"|location('07)<br />
!width="35"|Vector<br />
!width="40"|Responsible<br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091001 K091001] ||lsrR ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091002 K091002] ||lsrK ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091106 K091106] ||plsrA/cI ||MW ||N/A ||N/A || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=Test_pLasR/cI&diff=5925
Test pLasR/cI
2008-07-11T14:54:58Z
<p>XiZhu: </p>
<hr />
<div>pLasR/cI is constitutively off and is repressed by cI. The addition of p-AI-1, activates LasR causing the promoter to turn on. The addition of cI to should repress the promoter causing it to turn off.<br />
<br />
{| border="1" cellpadding="2"<br />
!width="30"|LasR<br />
!width="30"|cI<br />
!width="30"|p-AI-1 + GFP<br />
!width="30"|p-AI-1 - GFP<br />
|-<br />
| 0 || 0 || 0 || 0 <br />
|-<br />
| 1 || 0 || 1 || 0 <br />
|-<br />
| 0 || 1 || 0 || 0 <br />
|-<br />
| 1 || 1 || 0 || 0 <br />
|}<br />
<br />
<br />
'''Test pLasR/cI:'''<br />
<br />
pBAD→RBS+LasR+TT (from James DC)<br />
<br />
I13453+B0034+C0079+B0015<br />
<br />
plasR/cI→RBS+GFP+tt <br />
<br />
K09110?+E0240 does not glow<br />
<br />
'''add P-AI-1'''<br />
<br />
pBAD→RBS+LasR+TT (from James DC)<br />
<br />
I13453+B0034+C0079+B0015<br />
<br />
plasR/cI→RBS+GFP+tt <br />
<br />
K09110?+E0240 glows<br />
<br />
<br />
'''Ligation plan for test sequences'''<br />
<br />
Digest plasR/cI with S/P, E0240 with X/P; do ligation.</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=Test_pLsrA/cI&diff=5924
Test pLsrA/cI
2008-07-11T14:54:18Z
<p>XiZhu: </p>
<hr />
<div>Test pLsrA/cI promoter<br />
<br />
The pLsrA/cI promoter is constitutively off and is repressed by the lsrR protein. The addition of AI-2 should de-repress the lsrR operon causing it to glow. The addition of the cI protein should repress the promoter, causing it to not glow.<br />
<br />
<br />
''' Test pLsrA/cI repression:'''<br />
<br />
pBAD→RBS+lsrK+RBS+LsrR+TT<br />
<br />
plsrA/cI→RBS+GFP+TT does not glow<br />
<br />
[http://partsregistry.org/Part:BBa_I13453 I13453]+[http://partsregistry.org/Part:BBa_B0034 B0034]+[http://partsregistry.org/Part:BBa_K091002 K091002]+[http://partsregistry.org/Part:BBa_B0034 B0034]+[http://partsregistry.org/Part:BBa_K091001 K091001]+[http://partsregistry.org/Part:BBa_B0015 B0015]<br />
<br />
[http://partsregistry.org/Part:BBa_K091106 K091106]+[http://partsregistry.org/Part:BBa_E0240 E0240]<br />
<br />
add AI-2<br />
<br />
pBAD→RBS+lsrK+RBS+LsrR+TT<br />
<br />
plsrA/cI→RBS+GFP+TT glows<br />
<br />
[http://partsregistry.org/Part:BBa_I13453 I13453]+[http://partsregistry.org/Part:BBa_B0034 B0034]+[http://partsregistry.org/Part:BBa_K091002 K091002]+[http://partsregistry.org/Part:BBa_B0034 B0034]+[http://partsregistry.org/Part:BBa_K091001 K091001]+[http://partsregistry.org/Part:BBa_B0015 B0015]<br />
<br />
[http://partsregistry.org/Part:BBa_K091106 K091106]+[http://partsregistry.org/Part:BBa_E0240 E0240]<br />
<br />
{| border="1" cellpadding="2"<br />
!width="30"|LsrR<br />
!width="30"|cI<br />
!width="30"|AI-2 + GFP<br />
!width="30"|AI-2 - GFP<br />
|-<br />
| 0 || 0 || 1 || 1 <br />
|-<br />
| 1 || 0 || 1 || 0 <br />
|-<br />
| 0 || 1 || 0 || 0 <br />
|-<br />
| 1 || 1 || 0 || 0 <br />
|}<br />
<br />
<br />
'''Ligation plan for test sequences'''<br />
<br />
Digest RBS with S/P, lsrK with X/P; do ligation.<br />
<br />
Digest RBS with S/P, lsrR with X/P; do ligation.<br />
<br />
Digest pBAD with S/P, RBS+lsrK with X/P; do ligation.<br />
<br />
Digest TT with R/X, RBS+lsrR with R/S; do ligation.<br />
<br />
Digest RBS+lsrR+TT with R/X, pBAD+RBS+lsrK with R/S,; do ligation.<br />
<br />
Digest plsrA/cI with S/P, E0240 with X/P; do ligation.</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=Test_pLasR/cI&diff=5923
Test pLasR/cI
2008-07-11T14:53:22Z
<p>XiZhu: </p>
<hr />
<div>pLasR/cI is constitutively off and is repressed by cI. The addition of p-AI-1, activates LasR causing the promoter to turn on. The addition of cI to should repress the promoter causing it to turn off.<br />
<br />
{| border="1" cellpadding="2"<br />
!width="30"|LasR<br />
!width="30"|cI<br />
!width="30"|p-AI-1 + GFP<br />
!width="30"|p-AI-1 - GFP<br />
|-<br />
| 0 || 0 || 0 || 0 <br />
|-<br />
| 1 || 0 || 1 || 0 <br />
|-<br />
| 0 || 1 || 0 || 0 <br />
|-<br />
| 1 || 1 || 0 || 0 <br />
|}<br />
<br />
<br />
'''Test pLasR/cI:'''<br />
<br />
pBAD→RBS+LasR+TT (from James DC)<br />
<br />
I13453+B0034+C0079+B0015<br />
<br />
plasR/cI→RBS+GFP <br />
<br />
K09110?+E0240 does not glow<br />
<br />
'''add P-AI-1'''<br />
<br />
pBAD→RBS+LasR+TT (from James DC)<br />
<br />
I13453+B0034+C0079+B0015<br />
<br />
plasR/cI→RBS+GFP <br />
<br />
K09110?+E0240 glows<br />
<br />
<br />
'''Ligation plan for test sequences'''<br />
<br />
<br />
K09110? ligated with E0240<br />
<br />
Digest plasR/cI with S/P, E0240 with X/P; do ligation.</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=Test_pLsrA/cI&diff=5922
Test pLsrA/cI
2008-07-11T14:52:19Z
<p>XiZhu: </p>
<hr />
<div>Test pLsrA/cI promoter<br />
<br />
The pLsrA/cI promoter is constitutively off and is repressed by the lsrR protein. The addition of AI-2 should de-repress the lsrR operon causing it to glow. The addition of the cI protein should repress the promoter, causing it to not glow.<br />
<br />
<br />
''' Test pLsrA/cI repression:'''<br />
<br />
pBAD→RBS+lsrK+RBS+LsrR+TT<br />
<br />
plsrA/cI→GFP does not glow<br />
<br />
[http://partsregistry.org/Part:BBa_I13453 I13453]+[http://partsregistry.org/Part:BBa_B0034 B0034]+[http://partsregistry.org/Part:BBa_K091002 K091002]+[http://partsregistry.org/Part:BBa_B0034 B0034]+[http://partsregistry.org/Part:BBa_K091001 K091001]+[http://partsregistry.org/Part:BBa_B0015 B0015]<br />
<br />
[http://partsregistry.org/Part:BBa_K091106 K091106]+[http://partsregistry.org/Part:BBa_E0240 E0240]<br />
<br />
add AI-2<br />
<br />
pBAD→RBS+lsrK+RBS+LsrR+TT<br />
<br />
plsrA/cI→GFP glows<br />
<br />
[http://partsregistry.org/Part:BBa_I13453 I13453]+[http://partsregistry.org/Part:BBa_B0034 B0034]+[http://partsregistry.org/Part:BBa_K091002 K091002]+[http://partsregistry.org/Part:BBa_B0034 B0034]+[http://partsregistry.org/Part:BBa_K091001 K091001]+[http://partsregistry.org/Part:BBa_B0015 B0015]<br />
<br />
[http://partsregistry.org/Part:BBa_K091106 K091106]+[http://partsregistry.org/Part:BBa_E0240 E0240]<br />
<br />
{| border="1" cellpadding="2"<br />
!width="30"|LsrR<br />
!width="30"|cI<br />
!width="30"|AI-2 + GFP<br />
!width="30"|AI-2 - GFP<br />
|-<br />
| 0 || 0 || 1 || 1 <br />
|-<br />
| 1 || 0 || 1 || 0 <br />
|-<br />
| 0 || 1 || 0 || 0 <br />
|-<br />
| 1 || 1 || 0 || 0 <br />
|}<br />
<br />
<br />
'''Ligation plan for test sequences'''<br />
<br />
<br />
K091106 ligated with E0240<br />
<br />
Digest RBS with S/P, lsrK with X/P; do ligation.<br />
<br />
Digest RBS with S/P, lsrR with X/P; do ligation.<br />
<br />
Digest pBAD with S/P, RBS+lsrK with X/P; do ligation.<br />
<br />
Digest TT with R/X, RBS+lsrR with R/S; do ligation.<br />
<br />
Digest RBS+lsrR+TT with R/X, pBAD+RBS+lsrK with R/S,; do ligation.<br />
<br />
Digest plsrA/cI with S/P, E0240 with X/P; do ligation.</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=Test_pLsrA/cI&diff=5921
Test pLsrA/cI
2008-07-11T14:51:59Z
<p>XiZhu: </p>
<hr />
<div>Test pLsrA/cI promoter<br />
<br />
The pLsrA/cI promoter is constitutively off and is repressed by the lsrR protein. The addition of AI-2 should de-repress the lsrR operon causing it to glow. The addition of the cI protein should repress the promoter, causing it to not glow.<br />
<br />
<br />
''' Test pLsrA/cI repression:'''<br />
<br />
pBAD→RBS+lsrK+RBS+LsrR+TT<br />
<br />
plsrA/cI→GFP does not glow<br />
<br />
[http://partsregistry.org/Part:BBa_I13453 I13453]+[http://partsregistry.org/Part:BBa_B0034 B0034]+[http://partsregistry.org/Part:BBa_K091002 K091002]+[http://partsregistry.org/Part:BBa_B0034 B0034]+[http://partsregistry.org/Part:BBa_K091001 K091001]+[http://partsregistry.org/Part:BBa_B0015 B0015]<br />
<br />
[http://partsregistry.org/Part:BBa_K091106 K091106]+[http://partsregistry.org/Part:BBa_E0240 E0240]<br />
<br />
add AI-2<br />
<br />
pBAD→RBS+lsrK+RBS+LsrR+TT<br />
<br />
plsrA/cI→GFP glows<br />
<br />
[http://partsregistry.org/Part:BBa_I13453 I13453]+[http://partsregistry.org/Part:BBa_B0034 B0034]+[http://partsregistry.org/Part:BBa_K091002 K091002]+[http://partsregistry.org/Part:BBa_B0034 B0034]+[http://partsregistry.org/Part:BBa_K091001 K091001]+[http://partsregistry.org/Part:BBa_B0015 B0015]<br />
<br />
[http://partsregistry.org/Part:BBa_K091106 K091106]+[http://partsregistry.org/Part:BBa_E0240 E0240]<br />
<br />
{| border="1" cellpadding="2"<br />
!width="30"|LsrR<br />
!width="30"|cI<br />
!width="30"|AI-2 + GFP<br />
!width="30"|AI-2 - GFP<br />
|-<br />
| 0 || 0 || 1 || 1 <br />
|-<br />
| 1 || 0 || 1 || 0 <br />
|-<br />
| 0 || 1 || 0 || 0 <br />
|-<br />
| 1 || 1 || 0 || 0 <br />
|}<br />
<br />
<br />
'''Ligation plan for test sequences'''<br />
<br />
<br />
K091106 ligated with E0240<br />
<br />
Digest RBS with S/P, lsrK with X/P; do ligation.<br />
Digest RBS with S/P, lsrR with X/P; do ligation.<br />
Digest pBAD with S/P, RBS+lsrK with X/P; do ligation.<br />
Digest TT with R/X, RBS+lsrR with R/S; do ligation.<br />
Digest RBS+lsrR+TT with R/X, pBAD+RBS+lsrK with R/S,; do ligation.<br />
Digest plsrA/cI with S/P, E0240 with X/P; do ligation.</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=Test_pLasR/cI&diff=5920
Test pLasR/cI
2008-07-11T14:42:37Z
<p>XiZhu: </p>
<hr />
<div>pLasR/cI is constitutively off and is repressed by cI. The addition of p-AI-1, activates LasR causing the promoter to turn on. The addition of cI to should repress the promoter causing it to turn off.<br />
<br />
{| border="1" cellpadding="2"<br />
!width="30"|LasR<br />
!width="30"|cI<br />
!width="30"|p-AI-1 + GFP<br />
!width="30"|p-AI-1 - GFP<br />
|-<br />
| 0 || 0 || 0 || 0 <br />
|-<br />
| 1 || 0 || 1 || 0 <br />
|-<br />
| 0 || 1 || 0 || 0 <br />
|-<br />
| 1 || 1 || 0 || 0 <br />
|}<br />
<br />
<br />
'''Test pLasR/cI:'''<br />
<br />
pBAD→RBS+LasR+TT (from James DC)<br />
<br />
I13453+B0034+C0079+B0015<br />
<br />
plasR/cI→RBS+GFP <br />
<br />
K09110?+E0240 does not glow<br />
<br />
'''add P-AI-1'''<br />
<br />
pBAD→RBS+LasR+TT (from James DC)<br />
<br />
I13453+B0034+C0079+B0015<br />
<br />
plasR/cI→RBS+GFP <br />
<br />
K09110?+E0240 glows<br />
<br />
<br />
<br />
'''Ligation plan for test sequences'''<br />
<br />
<br />
K09110? ligated with E0240<br />
<br />
Digest RBS with S/P, E0240 with X/P; do ligation.<br />
<br />
Digest plasR/cI with S/P, RBS+E0240 with X/P; do ligation.</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LasR/cI&diff=5888
LasR/cI
2008-07-10T11:48:04Z
<p>XiZhu: </p>
<hr />
<div>pBAD+RBS+lasR+RBS+cI+TT+pLasR/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+C0079+B0034+C0051+B0034+C0061+B0034+C0072+B0015</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5866
LsrA/cI
2008-07-08T22:11:15Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': <br />
<br />
'''pBAD+RBS+lsrK+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT'''<br />
<br />
'''I13453'''+'''B0034'''+'''K091002'''+'''B0034'''+'''K091001'''+'''P0455'''(B0034+C0051+B0015)+'''K091106'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| B0034 ||RBS ||R/X; S/P||S/P || 12||K091001||lsrR ||R/X;S/P ||X/P ||120 ||TBD ||<br />
|-<br />
| 2|| B0034 ||RBS ||R/X; S/P||S/P || 12||K091002||lsrK ||R/X;S/P ||X/P ||516 ||TBD ||<br />
|-<br />
| 3||I13453||pBAD ||R/X;S/P ||S/P || 120||TBD||RBS+lsrK ||R/X; S/P ||X/P || ||TBD || <br />
|-<br />
| 4||TBD||pBAD+RBS+lsrK||R/X; S/P ||S/P || ||TBD ||RBS+lsrR ||R/X; S/P ||X/P || ||TBD ||<br />
|-<br />
| 5||P0455 ||RBS+cI+TT ||R/X; S/P ||R/X ||896 ||TBD ||pBAD+RBS+lsrK+RBS+lsrR ||R/X; S/P ||R/S || ||TBD ||<br />
|-<br />
| 6|| K091106 ||pLsrA/cI ||R/X; S/P ||S/P || 141||C0261||RBS+luxI ||R/X; S/P ||X/P ||661 ||TBD||<br />
|-<br />
| 7||B0015 ||TT ||R/X; S/P ||R/X ||129 ||I13025 ||RBS+Mnt ||R/X;S/P ||R/S ||331 ||TBD ||<br />
|-<br />
| 8||TBD ||RBS+Mnt+TT ||R/X; S/P ||R/X || ||TBD ||pLsrA/cI+RBS+luxI ||R/X; S/P ||R/S || || TBD||<br />
|-<br />
| 9|| TBD ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||R/X || ||TBD||pBAD+RBS+lsrK+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||R/S || ||TBD ||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="110"|Description<br />
!width="60"|Source<br />
!width="100"|location('07)<br />
!width="35"|Vector<br />
!width="40"|Responsible<br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_P0455 P0455]||RBS+cI+TT ||registry 07 ||Plt.1-16M ||pSB1AC3 || <br />
|-<br />
[http://partsregistry.org/Part:BBa_P0451 P0451]||RBS+cI+TT ||registry 07 ||Plt.1-21O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091001 K091001] ||lsrR ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091002 K091002] ||lsrK ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091106 K091106] ||plsrA/cI ||MW ||N/A ||N/A || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5865
LsrA/cI
2008-07-08T22:09:56Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': <br />
<br />
'''pBAD+RBS+lsrK+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT'''<br />
<br />
'''I13453'''+'''B0034'''+'''K091002'''B0034'''+'''K091001'''+'''P0455'''(B0034+C0051+B0015)+'''K091106'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| B0034 ||RBS ||R/X; S/P||S/P || 12||K091001||lsrR ||R/X;S/P ||X/P ||120 ||TBD ||<br />
|-<br />
| 2|| B0034 ||RBS ||R/X; S/P||S/P || 12||K091002||lsrK ||R/X;S/P ||X/P ||516 ||TBD ||<br />
|-<br />
| 3||I13453||pBAD ||R/X;S/P ||S/P || 120||TBD||RBS+lsrK ||R/X; S/P ||X/P || ||TBD || <br />
|-<br />
| 4||TBD||pBAD+RBS+lsrK||R/X; S/P ||S/P || ||TBD ||RBS+lsrR ||R/X; S/P ||X/P || ||TBD ||<br />
|-<br />
| 5||P0455 ||RBS+cI+TT ||R/X; S/P ||R/X ||896 ||TBD ||pBAD+RBS+lsrK+RBS+lsrR ||R/X; S/P ||R/S || ||TBD ||<br />
|-<br />
| 6|| K091106 ||pLsrA/cI ||R/X; S/P ||S/P || 141||C0261||RBS+luxI ||R/X; S/P ||X/P ||661 ||TBD||<br />
|-<br />
| 7||B0015 ||TT ||R/X; S/P ||R/X ||129 ||I13025 ||RBS+Mnt ||R/X;S/P ||R/S ||331 ||TBD ||<br />
|-<br />
| 8||TBD ||RBS+Mnt+TT ||R/X; S/P ||R/X || ||TBD ||pLsrA/cI+RBS+luxI ||R/X; S/P ||R/S || || TBD||<br />
|-<br />
| 9|| TBD ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||R/X || ||TBD||pBAD+RBS+lsrK+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||R/S || ||TBD ||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="110"|Description<br />
!width="60"|Source<br />
!width="100"|location('07)<br />
!width="35"|Vector<br />
!width="40"|Responsible<br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_P0455 P0455]||RBS+cI+TT ||registry 07 ||Plt.1-16M ||pSB1AC3 || <br />
|-<br />
[http://partsregistry.org/Part:BBa_P0451 P0451]||RBS+cI+TT ||registry 07 ||Plt.1-21O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091001 K091001] ||lsrR ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091002 K091002] ||lsrK ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091106 K091106] ||plsrA/cI ||MW ||N/A ||N/A || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5862
LsrA/cI
2008-07-08T18:53:01Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': <br />
<br />
'''pBAD+RBS+lsrK+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT'''<br />
<br />
'''I13453'''+'''B0034'''+'''K091002'''B0034'''+'''K091001'''+'''P0455'''(B0034+C0051+B0015)+'''K091106'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| B0034 ||RBS ||R/X; S/P||S/P || 12||K091001||lsrR ||R/X;S/P ||R/S ||120 ||TBD ||<br />
|-<br />
| 1|| B0034 ||RBS ||R/X; S/P||R/X || 12||I13453||pBAD ||R/X;S/P ||R/S ||120 ||TBD ||<br />
|-<br />
| 2||TBD||pBAD+RBS ||R/X;S/P ||S/P || ||K091002||lsrK ||R/X; S/P ||X/P ||954 ||TBD || <br />
|-<br />
| 3||P0455 ||RBS+cI+TT ||R/X; S/P ||R/X ||896 ||TBD ||pBAD+RBS+lsrR ||R/X; S/P ||R/S || ||TBD ||<br />
|-<br />
| 4|| K091106 ||pLsrA/cI ||R/X; S/P ||S/P || 141||C0261||RBS+luxI ||R/X; S/P ||X/P ||661 ||TBD||<br />
|-<br />
| 5||B0015 ||TT ||R/X; S/P ||R/X ||129 ||I13025 ||RBS+Mnt ||R/X;S/P ||R/S ||331 ||TBD ||<br />
|-<br />
| 6||TBD ||RBS+Mnt+TT ||R/X; S/P ||R/X || ||TBD ||pLsrA/cI+RBS+luxI ||R/X; S/P ||R/S || || TBD||<br />
|-<br />
| 7|| TBD ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||R/X || ||TBD||pBAD+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||R/S || ||TBD ||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="110"|Description<br />
!width="60"|Source<br />
!width="100"|location('07)<br />
!width="35"|Vector<br />
!width="40"|Responsible<br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_P0455 P0455]||RBS+cI+TT ||registry 07 ||Plt.1-16M ||pSB1AC3 || <br />
|-<br />
[http://partsregistry.org/Part:BBa_P0451 P0451]||RBS+cI+TT ||registry 07 ||Plt.1-21O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091001 K091001] ||lsrR ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091106 K091106] ||plsrA/cI ||MW ||N/A ||N/A || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5753
LsrA/cI
2008-07-02T20:36:33Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': <br />
<br />
'''pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT'''<br />
<br />
'''I13453'''+'''B0034'''+'''K091001'''+'''P0455'''(B0034+C0051+B0015)+'''K091106'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| B0034 ||RBS ||R/X; S/P||S/P || 12||I13453||pBAD ||R/X;S/P ||X/P ||120 ||TBD ||<br />
|-<br />
| 2||TBD||pBAD+RBS ||R/X;S/P ||R/X || ||K091001||lsrR ||R/X; S/P ||R/S ||954 ||TBD || <br />
|-<br />
| 3||P0455 ||RBS+cI+TT ||R/X; S/P ||S/P ||896 ||TBD ||pBAD+RBS+lsrR ||R/X; S/P ||X/P || ||TBD ||<br />
|-<br />
| 4|| K091106 ||pLsrA/cI ||R/X; S/P ||R/X || 141||C0261||RBS+luxI ||R/X; S/P ||R/S ||661 ||TBD||<br />
|-<br />
| 5||B0015 ||TT ||R/X; S/P ||S/P ||129 ||I13025 ||RBS+Mnt ||R/X;S/P ||X/P ||331 ||TBD ||<br />
|-<br />
| 6||TBD ||RBS+Mnt+TT ||R/X; S/P ||S/P || ||TBD ||pLsrA/cI+RBS+luxI ||R/X; S/P ||X/P || || TBD||<br />
|-<br />
| 7|| TBD ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||S/P || ||TBD||pBAD+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||X/P || ||TBD ||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="110"|Description<br />
!width="60"|Source<br />
!width="100"|location('07)<br />
!width="35"|Vector<br />
!width="40"|Responsible<br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_P0455 P0455]||RBS+cI+TT ||registry 07 ||Plt.1-16M ||pSB1AC3 || <br />
|-<br />
[http://partsregistry.org/Part:BBa_P0451 P0451]||RBS+cI+TT ||registry 07 ||Plt.1-21O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091001 K091001] ||lsrR ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091106 K091106] ||plsrA/cI ||MW ||N/A ||N/A || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5752
LsrA/cI
2008-07-02T20:36:19Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': <br />
<br />
'''pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT'''<br />
<br />
'''I13453'''+'''B0034'''+'''K091001'''+'''P0455'''(B0034+C0051+B0015)+'''K091106'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| B0034 ||RBS ||R/X; S/P||S/P || 12||I13453||pBAD ||R/X;S/P ||X/P ||120 ||TBD ||<br />
|-<br />
| 2||TBD||pBAD+RBS ||R/X;S/P ||R/X || ||K091001||lsrR ||R/X; S/P ||R/S ||954 ||TBD || <br />
|-<br />
| 3||P0455 ||RBS+cI+TT ||R/X; S/P ||S/P ||896 ||TBD ||pBAD+RBS+lsrR ||R/X; S/P ||X/P || ||TBD ||<br />
|-<br />
| 4|| K091106 ||pLsrA/cI ||R/X; S/P ||R/X || 141||C0261||RBS+luxI ||R/X; S/P ||R/S ||661 ||TBD||<br />
|<br />
| 5||B0015 ||TT ||R/X; S/P ||S/P ||129 ||I13025 ||RBS+Mnt ||R/X;S/P ||X/P ||331 ||TBD ||<br />
|-<br />
| 6||TBD ||RBS+Mnt+TT ||R/X; S/P ||S/P || ||TBD ||pLsrA/cI+RBS+luxI ||R/X; S/P ||X/P || || TBD||<br />
|-<br />
| 7|| TBD ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||S/P || ||TBD||pBAD+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||X/P || ||TBD ||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="110"|Description<br />
!width="60"|Source<br />
!width="100"|location('07)<br />
!width="35"|Vector<br />
!width="40"|Responsible<br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_P0455 P0455]||RBS+cI+TT ||registry 07 ||Plt.1-16M ||pSB1AC3 || <br />
|-<br />
[http://partsregistry.org/Part:BBa_P0451 P0451]||RBS+cI+TT ||registry 07 ||Plt.1-21O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091001 K091001] ||lsrR ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091106 K091106] ||plsrA/cI ||MW ||N/A ||N/A || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5750
LsrA/cI
2008-07-02T20:35:41Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': <br />
<br />
'''pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT'''<br />
<br />
'''I13453'''+'''B0034'''+'''K091001'''+'''P0455'''(B0034+C0051+B0015)+'''K091106'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| B0034 ||RBS ||R/X; S/P||S/P || 12||I13453||pBAD ||R/X;S/P ||X/P ||120 ||TBD ||<br />
|-<br />
| 2||TBD||pBAD+RBS ||R/X;S/P ||R/X || ||K091001||lsrR ||R/X; S/P ||R/S ||954 ||TBD || <br />
|-<br />
| 3||P0455 ||RBS+cI+TT ||R/X; S/P ||S/P ||896 ||TBD ||pBAD+RBS+lsrR ||R/X; S/P ||X/P || ||TBD ||<br />
|-<br />
| 4|| K091106 ||pLsrA/cI ||R/X; S/P ||R/X || 141||C0261||RBS+luxI ||R/X; S/P ||R/S ||661 ||TBD||<br />
|S<br />
| 5||B0015 ||TT ||R/X; S/P ||S/P ||129 ||I13025 ||RBS+Mnt ||R/X;S/P ||X/P ||331 ||TBD ||<br />
|-<br />
| 6||TBD ||RBS+Mnt+TT ||R/X; S/P ||S/P || ||TBD ||pLsrA/cI+RBS+luxI ||R/X; S/P ||X/P || || TBD||<br />
|-<br />
| 7|| TBD ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||S/P || ||TBD||pBAD+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||X/P || ||TBD ||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="110"|Description<br />
!width="60"|Source<br />
!width="100"|location('07)<br />
!width="35"|Vector<br />
!width="40"|Responsible<br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_P0455 P0455]||RBS+cI+TT ||registry 07 ||Plt.1-16M ||pSB1AC3 || <br />
|-<br />
[http://partsregistry.org/Part:BBa_P0451 P0451]||RBS+cI+TT ||registry 07 ||Plt.1-21O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091001 K091001] ||lsrR ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091106 K091106] ||plsrA/cI ||MW ||N/A ||N/A || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5711
LsrA/cI
2008-07-02T15:46:29Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': <br />
<br />
'''pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT'''<br />
<br />
'''I13453'''+'''B0034'''+'''K091001'''+'''P0455'''(B0034+C0051+B0015)+'''K091106'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12||TBD ||<br />
|-<br />
| 2||K091001||lsrR ||R/X; S/P ||R/X ||954 ||TBD ||pBAD+RBS ||R/X;S/P ||R/S || || TBD||<br />
|-<br />
| 3||TBD ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 ||TBD ||<br />
|-<br />
| 4|| C0261||RBS+luxI ||R/X; S/P ||R/X ||661 ||K091106 ||pLsrA/cI ||R/X; S/P ||R/S || 141||TBD||<br />
|-<br />
| 5||I13025 ||RBS+Mnt ||R/X;S/P ||S/P ||331 ||B0015 ||TT ||R/X; S/P ||X/P ||129 ||TBD ||<br />
|-<br />
| 6||TBD ||pLsrA/cI+RBS+luxI ||R/X; S/P ||S/P || ||TBD ||RBS+Mnt+TT ||R/X; S/P ||X/P || || TBD||<br />
|-<br />
| 7|| TBD||pBAD+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||S/P || ||TBD ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||X/P || ||TBD ||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="110"|Description<br />
!width="60"|Source<br />
!width="100"|location('07)<br />
!width="35"|Vector<br />
!width="40"|Responsible<br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_P0455 P0455]||RBS+cI+TT ||registry 07 ||Plt.1-16M ||pSB1AC3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091001 K091001] ||lsrR ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091106 K091106] ||plsrA/cI ||MW ||N/A ||N/A || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5704
LsrA/cI
2008-07-02T15:27:36Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': <br />
<br />
'''pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT'''<br />
<br />
'''I13453'''+'''B0034'''+'''K091001'''+'''P0455'''(B0034+C0051+B0015)+'''K091106'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||<br />
|-<br />
| 2||K091001||lsrR ||R/X; S/P ||R/X ||954 || ||pBAD+RBS ||R/X;S/P ||R/S || || ||<br />
|-<br />
| 3|| ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || ||<br />
|-<br />
| 4|| C0261||RBS+luxI ||R/X; S/P ||R/X ||661 ||K091106 ||pLsrA/cI ||R/X; S/P ||R/S || 141|| ||<br />
|-<br />
| 5||I13025 ||RBS+Mnt ||R/X;S/P ||S/P ||331 ||B0015 ||TT ||R/X; S/P ||X/P ||129 || ||<br />
|-<br />
| 6|| ||pLsrA/cI+RBS+luxI ||R/X; S/P ||S/P || || ||RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
| 7|| ||pBAD+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||S/P || || ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="110"|Description<br />
!width="60"|Source<br />
!width="100"|location('07)<br />
!width="35"|Vector<br />
!width="40"|Responsible<br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_P0455 P0455]||RBS+cI+TT ||registry 07 ||Plt.1-16M ||pSB1AC3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091001 K091001] ||lsrR ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091106 K091106] ||plsrA/cI ||MW ||N/A ||N/A || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5702
LsrA/cI
2008-07-02T15:26:28Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+K091001+P0455(B0034+C0051+B0015)+K091106+C0261(B0034+C0061)+I13025(B0034+C0072)+B0015<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||<br />
|-<br />
| 2||K091001||lsrR ||R/X; S/P ||R/X ||954 || ||pBAD+RBS ||R/X;S/P ||R/S || || ||<br />
|-<br />
| 3|| ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || ||<br />
|-<br />
| 4|| C0261||RBS+luxI ||R/X; S/P ||R/X ||661 ||K091106 ||pLsrA/cI ||R/X; S/P ||R/S || 141|| ||<br />
|-<br />
| 5||I13025 ||RBS+Mnt ||R/X;S/P ||S/P ||331 ||B0015 ||TT ||R/X; S/P ||X/P ||129 || ||<br />
|-<br />
| 6|| ||pLsrA/cI+RBS+luxI ||R/X; S/P ||S/P || || ||RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
| 7|| ||pBAD+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||S/P || || ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="110"|Description<br />
!width="60"|Source<br />
!width="100"|location('07)<br />
!width="35"|Vector<br />
!width="40"|Responsible<br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_P0455 P0455]||RBS+cI+TT ||registry 07 ||Plt.1-16M ||pSB1AC3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091001 K091001] ||lsrR ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091106 K091106] ||plsrA/cI ||MW ||N/A ||N/A || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5701
LsrA/cI
2008-07-02T15:24:44Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||<br />
|-<br />
| 2||K091001||lsrR ||R/X; S/P ||R/X ||954 || ||pBAD+RBS ||R/X;S/P ||R/S || || ||<br />
|-<br />
| 3|| ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || ||<br />
|-<br />
| 4|| C0261||RBS+luxI ||R/X; S/P ||R/X ||661 ||K091106 ||pLsrA/cI ||R/X; S/P ||R/S || 141|| ||<br />
|-<br />
| 5||I13025 ||RBS+Mnt ||R/X;S/P ||S/P ||331 ||B0015 ||TT ||R/X; S/P ||X/P ||129 || ||<br />
|-<br />
| 6|| ||pLsrA/cI+RBS+luxI ||R/X; S/P ||S/P || || ||RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
| 7|| ||pBAD+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||S/P || || ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="110"|Description<br />
!width="60"|Source<br />
!width="100"|location('07)<br />
!width="35"|Vector<br />
!width="40"|Responsible<br />
|-<br />
| [http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_P0455 P0455]||RBS+cI+TT ||registry 07 ||Plt.1-16M ||pSB1AC3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091001 K091001] ||lsrR ||MW ||N/A||N/A || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_K091106 K091106] ||plsrA/cI ||MW ||N/A ||N/A || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5700
LsrA/cI
2008-07-02T15:19:01Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="110"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||<br />
|-<br />
| 2||K091001||lsrR ||R/X; S/P ||R/X ||954 || ||pBAD+RBS ||R/X;S/P ||R/S || || ||<br />
|-<br />
| 3|| ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || ||<br />
|-<br />
| 4|| C0261||RBS+luxI ||R/X; S/P ||R/X ||661 ||K091106 ||pLsrA/cI ||R/X; S/P ||R/S || 141|| ||<br />
|-<br />
| 5||I13025 ||RBS+Mnt ||R/X;S/P ||S/P ||331 ||B0015 ||TT ||R/X; S/P ||X/P ||129 || ||<br />
|-<br />
| 6|| ||pLsrA/cI+RBS+luxI ||R/X; S/P ||S/P || || ||RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
| 7|| ||pBAD+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||S/P || || ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="110"|Description<br />
!width="60"|Source<br />
!width="100"|location('07)<br />
!width="35"|Vector<br />
!width="40"|Responsible<br />
|-<br />
| [http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_P0455 P0455]||RBS+cI+TT ||registry 07 ||Plt.1-16M ||pSB1AC3 || <br />
|-<br />
| || || || || || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5699
LsrA/cI
2008-07-02T15:18:23Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||<br />
|-<br />
| 2||K091001||lsrR ||R/X; S/P ||R/X ||954 || ||pBAD+RBS ||R/X;S/P ||R/S || || ||<br />
|-<br />
| 3|| ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || ||<br />
|-<br />
| 4|| C0261||RBS+luxI ||R/X; S/P ||R/X ||661 ||K091106 ||pLsrA/cI ||R/X; S/P ||R/S || 141|| ||<br />
|-<br />
| 5||I13025 ||RBS+Mnt ||R/X;S/P ||S/P ||331 ||B0015 ||TT ||R/X; S/P ||X/P ||129 || ||<br />
|-<br />
| 6|| ||pLsrA/cI+RBS+luxI ||R/X; S/P ||S/P || || ||RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
| 7|| ||pBAD+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||S/P || || ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="100"|Description<br />
!width="50"|Source<br />
!width="100"|location('07)<br />
!width="30"|Vector<br />
!width="40"|Responsible<br />
|-<br />
| [http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_P0455 P0455]||RBS+cI+TT ||registry 07 ||Plt.1-16M ||pSB1AC3 || <br />
|-<br />
| || || || || || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5697
LsrA/cI
2008-07-02T15:18:01Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||<br />
|-<br />
| 2||K091001||lsrR ||R/X; S/P ||R/X ||954 || ||pBAD+RBS ||R/X;S/P ||R/S || || ||<br />
|-<br />
| 3|| ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || ||<br />
|-<br />
| 4|| C0261||RBS+luxI ||R/X; S/P ||R/X ||661 ||K091106 ||pLsrA/cI ||R/X; S/P ||R/S || 141|| ||<br />
|-<br />
| 5||I13025 ||RBS+Mnt ||R/X;S/P ||S/P ||331 ||B0015 ||TT ||R/X; S/P ||X/P ||129 || ||<br />
|-<br />
| 6|| ||pLsrA/cI+RBS+luxI ||R/X; S/P ||S/P || || ||RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
| 7|| ||pBAD+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||S/P || || ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="40"|BBa_<br />
!width="100"|Description<br />
!width="40"|Source<br />
!width="100"|location('07)<br />
!width="30"|Vector<br />
!width="40"|Responsible<br />
|-<br />
| [http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_P0455 P0455]||RBS+cI+TT ||registry 07 ||Plt.1-16M ||pSB1AC3 || <br />
|-<br />
| || || || || || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5696
LsrA/cI
2008-07-02T15:17:39Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||<br />
|-<br />
| 2||K091001||lsrR ||R/X; S/P ||R/X ||954 || ||pBAD+RBS ||R/X;S/P ||R/S || || ||<br />
|-<br />
| 3|| ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || ||<br />
|-<br />
| 4|| C0261||RBS+luxI ||R/X; S/P ||R/X ||661 ||K091106 ||pLsrA/cI ||R/X; S/P ||R/S || 141|| ||<br />
|-<br />
| 5||I13025 ||RBS+Mnt ||R/X;S/P ||S/P ||331 ||B0015 ||TT ||R/X; S/P ||X/P ||129 || ||<br />
|-<br />
| 6|| ||pLsrA/cI+RBS+luxI ||R/X; S/P ||S/P || || ||RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
| 7|| ||pBAD+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||S/P || || ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="30"|BBa_<br />
!width="100"|Description<br />
!width="30"|Source<br />
!width="100"|location('07)<br />
!width="30"|Vector<br />
!width="40"|Responsible<br />
|-<br />
| [http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_P0455 P0455]||RBS+cI+TT ||registry 07 ||Plt.1-16M ||pSB1AC3 || <br />
|-<br />
| || || || || || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5694
LsrA/cI
2008-07-02T15:16:30Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||<br />
|-<br />
| 2||K091001||lsrR ||R/X; S/P ||R/X ||954 || ||pBAD+RBS ||R/X;S/P ||R/S || || ||<br />
|-<br />
| 3|| ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || ||<br />
|-<br />
| 4|| C0261||RBS+luxI ||R/X; S/P ||R/X ||661 ||K091106 ||pLsrA/cI ||R/X; S/P ||R/S || 141|| ||<br />
|-<br />
| 5||I13025 ||RBS+Mnt ||R/X;S/P ||S/P ||331 ||B0015 ||TT ||R/X; S/P ||X/P ||129 || ||<br />
|-<br />
| 6|| ||pLsrA/cI+RBS+luxI ||R/X; S/P ||S/P || || ||RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
| 7|| ||pBAD+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||S/P || || ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="30"|BBa_<br />
!width="100"|Description<br />
!width="30"|Source<br />
!width="30"|Responsible<br />
!width="100"|location('07)<br />
!width="40"|Vector<br />
|-<br />
| [http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_P0455 P0455]||RBS+cI+TT ||registry 07 ||Plt.1-16M ||pSB1AC3 || <br />
|-<br />
| || || || || || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5690
LsrA/cI
2008-07-02T15:13:02Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||<br />
|-<br />
| 2||K091001||lsrR ||R/X; S/P ||R/X ||954 || ||pBAD+RBS ||R/X;S/P ||R/S || || ||<br />
|-<br />
| 3|| ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || ||<br />
|-<br />
| 4|| C0261||RBS+luxI ||R/X; S/P ||R/X ||661 ||K091106 ||pLsrA/cI ||R/X; S/P ||R/S || 141|| ||<br />
|-<br />
| 5||I13025 ||RBS+Mnt ||R/X;S/P ||S/P ||331 ||B0015 ||TT ||R/X; S/P ||X/P ||129 || ||<br />
|-<br />
| 6|| ||pLsrA/cI+RBS+luxI ||R/X; S/P ||S/P || || ||RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
| 7|| ||pBAD+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||S/P || || ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="30"|BBa_<br />
!width="100"|Description<br />
!width="30"|Source<br />
!width="30"|Responsible<br />
!width="80"|location('07)<br />
!width="40"|Vector<br />
|-<br />
| [http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_P0455 P0455]||RBS+cI+TT ||registry 07 ||Plt.1-16M ||pSB1AC3 || <br />
|-<br />
| || || || || || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5689
LsrA/cI
2008-07-02T15:12:42Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||<br />
|-<br />
| 2||K091001||lsrR ||R/X; S/P ||R/X ||954 || ||pBAD+RBS ||R/X;S/P ||R/S || || ||<br />
|-<br />
| 3|| ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || ||<br />
|-<br />
| 4|| C0261||RBS+luxI ||R/X; S/P ||R/X ||661 ||K091106 ||pLsrA/cI ||R/X; S/P ||R/S || 141|| ||<br />
|-<br />
| 5||I13025 ||RBS+Mnt ||R/X;S/P ||S/P ||331 ||B0015 ||TT ||R/X; S/P ||X/P ||129 || ||<br />
|-<br />
| 6|| ||pLsrA/cI+RBS+luxI ||R/X; S/P ||S/P || || ||RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
| 7|| ||pBAD+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||S/P || || ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="30"|BBa_<br />
!width="100"|Description<br />
!width="30"|Source<br />
!width="30"|Responsible<br />
!width="80"|location('07)<br />
!width="40"|Vector<br />
|-<br />
| [http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_B0034 B0034 ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_I13453 I13453 ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_C0261 C0261 ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || <br />
|-<br />
|[http://partsregistry.org/Part:BBa_P0455 P0455||RBS+cI+TT ||registry 07 ||Plt.1-16M ||pSB1AC3 || <br />
|-<br />
| || || || || || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5686
LsrA/cI
2008-07-02T15:03:45Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||<br />
|-<br />
| 2||K091001||lsrR ||R/X; S/P ||R/X ||954 || ||pBAD+RBS ||R/X;S/P ||R/S || || ||<br />
|-<br />
| 3|| ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || ||<br />
|-<br />
| 4|| C0261||RBS+luxI ||R/X; S/P ||R/X ||661 ||K091106 ||pLsrA/cI ||R/X; S/P ||R/S || 141|| ||<br />
|-<br />
| 5||I13025 ||RBS+Mnt ||R/X;S/P ||S/P ||331 ||B0015 ||TT ||R/X; S/P ||X/P ||129 || ||<br />
|-<br />
| 6|| ||pLsrA/cI+RBS+luxI ||R/X; S/P ||S/P || || ||RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
| 7|| ||pBAD+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||S/P || || ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="30"|BBa_<br />
!width="100"|Description<br />
!width="30"|Source<br />
!width="30"|Responsible<br />
!width="80"|location(07/08)<br />
!width="40"|Vector<br />
|-<br />
| || || || || || <br />
|-<br />
| || || || || || <br />
|-<br />
| || || || || || <br />
|-<br />
| || || || || || <br />
|-<br />
| || || || || || <br />
|-<br />
| || || || || || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5684
LsrA/cI
2008-07-02T15:03:00Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015<br />
<br />
<br />
'''Ligation Plan'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||<br />
|-<br />
| 2||K091001||lsrR ||R/X; S/P ||R/X ||954 || ||pBAD+RBS ||R/X;S/P ||R/S || || ||<br />
|-<br />
| 3|| ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || ||<br />
|-<br />
| 4|| C0261||RBS+luxI ||R/X; S/P ||R/X ||661 ||K091106 ||pLsrA/cI ||R/X; S/P ||R/S || 141|| ||<br />
|-<br />
| 5||I13025 ||RBS+Mnt ||R/X;S/P ||S/P ||331 ||B0015 ||TT ||R/X; S/P ||X/P ||129 || ||<br />
|-<br />
| 6|| ||pLsrA/cI+RBS+luxI ||R/X; S/P ||S/P || || ||RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
| 7|| ||pBAD+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||S/P || || ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
|}<br />
<br />
'''Parts'''<br />
{| border="1" cellpadding="2"<br />
!width="20"|BBa_<br />
!width="100"|Description<br />
!width="30"|Source<br />
!width="30"|Responsible<br />
!width="60"|location(07/08)<br />
!width="30"|Vector<br />
|-<br />
| || || || || || <br />
|-<br />
| || || || || || <br />
|-<br />
| || || || || || <br />
|-<br />
| || || || || || <br />
|-<br />
| || || || || || <br />
|-<br />
| || || || || || <br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5682
LsrA/cI
2008-07-02T14:57:32Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015<br />
<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||<br />
|-<br />
| 2||K091001||lsrR ||R/X; S/P ||R/X ||954 || ||pBAD+RBS ||R/X;S/P ||R/S || || ||<br />
|-<br />
| 3|| ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || ||<br />
|-<br />
| 4|| C0261||RBS+luxI ||R/X; S/P ||R/X ||661 ||K091106 ||pLsrA/cI ||R/X; S/P ||R/S || 141|| ||<br />
|-<br />
| 5||I13025 ||RBS+Mnt ||R/X;S/P ||S/P ||331 ||B0015 ||TT ||R/X; S/P ||X/P ||129 || ||<br />
|-<br />
| 6|| ||pLsrA/cI+RBS+luxI ||R/X; S/P ||S/P || || ||RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
| 7|| ||pBAD+RBS+lsrR+RBS+cI+TT ||R/X; S/P ||S/P || || ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5681
LsrA/cI
2008-07-02T14:56:43Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015<br />
<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||<br />
|-<br />
| 2||K091001||lsrR ||R/X; S/P ||R/X ||954 || ||pBAD+RBS ||R/X;S/P ||R/S || || ||<br />
|-<br />
| 3|| ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || ||<br />
|-<br />
| 4|| C0261||RBS+luxI ||R/X; S/P ||R/X ||661 ||K091106 ||pLsrA/cI ||R/X; S/P ||R/S || 141|| ||<br />
|-<br />
| 5||I13025 ||RBS+Mnt ||R/X;S/P ||S/P ||331 ||B0015 ||TT ||R/X; S/P ||X/P ||129 || ||<br />
|-<br />
| 6|| ||pLsrA/cI+RBS+luxI ||R/X; S/P ||S/P || || ||RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
| 7|| ||pBAD+RBS+lsrR+RBS+cI=TT ||R/X; S/P ||S/P || || ||pLsrA/cI+RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||X/P || || ||<br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5680
LsrA/cI
2008-07-02T14:50:15Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015<br />
<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||<br />
|-<br />
| 2||K091001||lsrR ||R/X; S/P ||R/X ||954 || ||pBAD+RBS ||R/X;S/P ||R/S || || ||<br />
|-<br />
| 3|| ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || ||<br />
|-<br />
| 4|| C0261||RBS+luxI ||R/X; S/P ||R/X ||661 ||K091106 ||pLsrA/cI ||R/X; S/P ||R/S || 141|| ||<br />
|-<br />
| 5||I13025 ||RBS+Mnt ||R/X;S/P ||S/P ||331 ||B0015 ||TT ||R/X; S/P ||X/P ||129 || ||<br />
|-<br />
| 6|| || || || || || || || || || || ||<br />
|-<br />
| 7|| || || || || || || || || || || ||<br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5679
LsrA/cI
2008-07-02T14:49:35Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015<br />
<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||<br />
|-<br />
| 2||K091001||lsrR ||R/X; S/P ||R/X ||954 || ||pBAD+RBS ||R/X;S/P ||R/S || || ||<br />
|-<br />
| 3|| ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || ||<br />
|-<br />
| 4|| C0261||RBS+luxI ||R/X; S/P ||R/X ||661 ||K091106 ||pLsrA/cI ||R/X; S/P ||R/S || || ||<br />
|-<br />
| 5||I13025 ||RBS+Mnt ||R/X;S/P ||S/P ||331 ||B0015 ||TT ||R/X; S/P ||X/P ||129 || ||<br />
|-<br />
| 6|| || || || || || || || || || || ||<br />
|-<br />
| 7|| || || || || || || || || || || ||<br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5678
LsrA/cI
2008-07-02T14:42:49Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015<br />
<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="100"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||<br />
|-<br />
| 2||K091106||lsrR ||R/X; S/P ||R/X ||954 || ||pBAD+RBS ||R/X;S/P ||R/S || || ||<br />
|-<br />
| 3|| ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5677
LsrA/cI
2008-07-02T14:42:28Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015<br />
<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="70"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="70"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||<br />
|-<br />
| 2||K091106||lsrR ||R/X; S/P ||R/X ||954 || ||pBAD+RBS ||R/X;S/P ||R/S || || ||<br />
|-<br />
| 3|| ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5675
LsrA/cI
2008-07-02T14:41:29Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015<br />
<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="70"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="70"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Prefix/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Prefix/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||<br />
|-<br />
| 2||K091106||lsrR ||R/X; S/P ||R/X ||954 || ||pBAD+RBS ||R/X;S/P ||R/S || || ||<br />
|-<br />
| 3|| ||pBAD+RBS+lsrR ||R/X; S/P ||S/P || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5671
LsrA/cI
2008-07-02T14:22:24Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015<br />
<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="70"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="70"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Prefix/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Prefix/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||<br />
|-<br />
| 2||K091106||lsrR ||R/X; S/P ||R/X ||954 || ||pBAD+RBS ||R/X;S/P ||R/S || || ||<br />
|-<br />
|3 ||C0051 || cI ||R/X;S/P ||S/P ||711 ||B0015 ||double terminator ||R/X;S/P ||X/P ||129 || ||<br />
|-<br />
| 4|| || cI+TT ||R/X;S/P ||R/X || || B0034||RBS ||R/X; S/P ||R/S ||12 || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5670
LsrA/cI
2008-07-02T14:16:48Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015<br />
<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="70"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="70"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Prefix/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Prefix/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||132<br />
|-<br />
| 2|| ||pBAD+RBS ||R/X; S/P ||S/P ||132 ||K091106 ||lsrR ||R/X;S/P ||X/P ||954 || ||1068<br />
|-<br />
|3 ||C0051 || cI ||R/X;S/P ||S/P ||711 ||B0015 ||double terminator ||R/X;S/P ||X/P ||129 || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5669
LsrA/cI
2008-07-02T14:13:56Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+TT+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+C0079+B0015+C0051+B0015+K091106+B0034+B0034+C0072+B0015<br />
<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="70"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="70"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Prefix/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Prefix/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||132<br />
|-<br />
| 2|| ||pBAD+RBS ||R/X; S/P ||S/P ||132 ||K091106 ||lsrR ||R/X;S/P ||X/P ||954 || ||1068<br />
|-<br />
|3 ||C0051 || cI ||R/X;S/P ||S/P ||711 ||B0015 ||double terminator ||R/X;S/P ||X/P ||129 || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5668
LsrA/cI
2008-07-02T14:13:20Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+TT+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+C0079+B0015+C0051+B0015+K091106+B0034+B0034+C0072+B0015<br />
<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="70"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="70"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Prefix/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Prefix/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| I13453||pBAD ||R/X;S/P ||S/P ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||<br />
|-<br />
| 2|| ||pBAD+RBS ||R/X; S/P ||S/P ||132 ||K091106 ||lsrR ||R/X;S/P ||X/P ||954 || ||<br />
|-<br />
|3 ||C0051 || cI ||R/X;S/P ||S/P ||711 ||B0015 ||double terminator ||R/X;S/P ||X/P ||129 || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5665
LsrA/cI
2008-07-02T14:05:33Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+TT+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+C0079+B0015+C0051+B0015+K091106+B0034+B0034+C0072+B0015<br />
<br />
{| border="1" cellpadding="2"<br />
!width="20"|<br />
!width="30"|Vector<br />
!width="70"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="70"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Prefix/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Prefix/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| K091001||LsrR ||R/X;S/P ||S/P ||954 ||B0015 ||double terminator ||R/X; S/P||X/P || 129|| ||<br />
|-<br />
|2 ||C0051 || cI ||R/X;S/P ||S/P ||711 ||B0015 ||double terminator ||R/X;S/P ||X/P ||129 || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
| || || || || || || || || || || || ||<br />
|-<br />
|}</div>
XiZhu
https://gcat.davidson.edu/GcatWiki/index.php?title=LsrA/cI&diff=5664
LsrA/cI
2008-07-02T14:05:14Z
<p>XiZhu: </p>
<hr />
<div>'''Goal''': pBAD+RBS+lsrR+TT+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT<br />
<br />
I13453+B0034+C0079+B0015+C0051+B0015+K091106+B0034+B0034+C0072+B0015<br />
<br />
{| border="1" cellpadding="2"<br />
!width="20"|Step<br />
!width="30"|Vector<br />
!width="70"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Insert<br />
!width="70"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|<br />
!width="30"|Product<br />
!width="30"|<br />
|-<br />
|.|| '''BBa_''' || '''Description''' ||''' Prefix/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Prefix/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''<br />
|-<br />
| 1|| K091001||LsrR ||R/X;S/P ||S/P ||954 ||B0015 ||double terminator ||R/X; S/P||X/P || 129|| ||<br />
|-<br />
|2 ||C0051 || cI ||R/X;S/P ||S/P ||711 ||B0015 ||double terminator ||R/X;S/P ||X/P ||129 || ||<br />
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|-<br />
|}</div>
XiZhu