View source for Blasting Spacers for Our Genome

== Blasting Spacers ==

CRISPR one results from CRISPR finder are shown below.  I blasted all of the spacers using the nr/nt database and the results are shown below.

[[Image:crisperOne.jpg]]


Spacer One: TGCGTCGTCCGGTGGCCGTCAATAAATGTCGCAAGGG

No significant hits. . . lowest E-value is 25

Matches by chance!

Spacer Two: TCCTACGACCTCGTCGGCGTCAACGGCTGGCCCGA

[[Image:spacerTwo.jpg]]

Spacer Three: CACCCTACAACAGGTGAAATCTACCAGACAAAAGA

No significant hits. . . lowest E-value is 0.36

Spacer Four: TCACCCAAGCGCAAGCAACAGCTGATCGAGGACCTG

No significant hits. . . lowest E-value is 0.36

Spacer Five: GCGACGGCGGCCAGTTCCGCGAGGGCGGGAAGGTCC

[[Image:spacerFive.jpg]]

This image is only part of it. . . 

[[Image:spacerFiveTwo.jpg]]


NOTE: lots of hits from each organism- indicates that multiple parts have been incorporated into our species' spacers. . . interesting to study what genes have been inserted

Spacer Six: TGCGAGTGTTGCGGGGAACCGACTCGGGTAGGCCAG

[[Image:spacerSix.jpg]]

No viral insertions in this spacer

Spacer Seven: ACGGTTTCGCTACCACCATCGCCACCAGCAACTGCCG

[[Image:spacerSeven.jpg]]

Spacer Eight: GACGAGTATGCCAACCGGCTCGTGAGCGGGCGC

[[Image:spacerEight.jpg]]

E-values not good- no significant hits!

Spacer Nine: TGGTAGGCGTCGTAGGTGTTCGTGGCGAGCGTGTC

[[Image:spacerNine.jpg]]

Spacer Ten: ACTCACTGGATTATGACCCCTACAACGAGGGCGTCA

No significant hits. . . lowest E-value is 1.5

Spacer Eleven: GGATCTCGATCGTTGTAGTATCCATAGCTGCTATACC

[[Image:spacerEleven.jpg]]

Spacer Twelve: GAAGTAACGCAACTCCAGTGAGCGCTACTGAGAGCCC

No significant hits. . . lowest E-value is 1.6

Spacer Thirteen:CCGATCACGCCCTGCCGATACTGGTAGTTCGCGATA

[[Image:spacer13.jpg]]

Spacer Fourteen: TCGTCGGCCGGCTCGTCGGCCGACGTGGACTTGC

[[Image:spacer14.jpg]]

Spacer Fifteen: AGTAGGTCTAATGTCTCTCTGTCGTCTATCAGCCCCG

No significant hits. . . lowest E-value is 1.6

Spacer Sixteen: GCTCTCCGGTGTCACAGGTCAGGTCACGGTCTCCGC

No significant hits . . . lowest E-value is 1.5

Spacer Seventeen: ACGGACAAGTCATCCACCCGCCAGTATCTCCCGGT

No significant hits . . . lowest E-value is 1.4

Spacer Eighteen: AAATACGATCCTGCGGTGACGCTACGTCCGGGGCAGC

No significant hits . . . lowest E-value is 6.4

Spacer Nineteen: CAGATGTGGGGTCTGTGGCCACAGTCTAACATCTCT

[[Image:spacer19.jpg]]

Spacer Twenty: CCTGATAACGGACTCTTGTAGGTCCGTTAGGTCGT

No significant hits . . . lowest E-value is 22

Spacer Twenty-One: AAAAATGAGTGACGTAGACATTCGGCAAAATGCCGG

No significant hits . . . lowest E-value is 6

Spacer Twenty-Two: CAGCAGCGAAACGAGCCGTCCGTCCTTTTGAGACA

No significant hits . . . lowest E-value is 1.2

Spacer Twenty-Three: GTCTAGCCCAGTCTGGTCGGGGTGGTCGGCAGGATCGG

No significant hits . . . lowest E-value is 0.43

Spacer Twenty-Four: CACTCCTCATATGTCTGTTCGAGCAGCGGGACGTG

No significant hits . . . lowest E-value is 1.4

Spacer Twenty-Five: ACCGTTGCCGCCGATCGGCAGCGAGCCGGTGATGTGT

[[Image:spacer25.jpg]]

Spacer Twenty-Six:  TCAAAGCGAGCCTCGAACGCGACGACGAAGATATG

[[Image:spacer26.jpg]]

Spacer Twenty 27: TCCTCCTTGTACCCACGGTCTTGCCGATCCATCCCG

No significant hits . . . lowest E-value is 6.0

Spacer Twenty-Eight: GACTGGCGTGTTGCCGTTCAGGCCGGCGTTGATCCCG

[[Image:spacer27.jpg]]

Spacer Twenty-Nine: CTCAGCAGCAGTCAACGGCATTTTATACACCTTGT

No significant hits . . . lowest E-value is 0.36

Spacer Thirty: CACCCCTTCCGGGGAGACGAGGAAACCCCGGACGA

No significant hits . . . lowest E-value is 1.4

Spacer Thirty-One: GTCACGCTGTCTGACGATATGGCTGACCAGGTGC

No significant hits . . . lowest E-value is 1.3

Spacer Thirty-Two: CACTCCTGGGCGGCCTCATCGGCGGCCATCGTC

[[Image:spacer32.jpg]]

Spacer Thirty-Three:GTTGTGTGAGGTATGCGATGGACACCACCGATCACG

No significant hits . . . lowest E-value is 6




CRISPR Two Spacers:

[[Image:crisprtwo.jpg]]
[[Image:crispr3.jpg]]

Spacer One: TCCGAGACGTGTTCCCTCTCTAGCTGTGCATCTTCC

No significant hits . . . lowest E-value is 0.36

Spacer Two: CAGATCTAAAACAATGTCATACGGAAAAATCGACATC

No significant hits . . . lowest E-value is 1.6

Spacer Three: GATCCGGAATATGAAGTGACGAACGATCCGGATACGG

No significant hits . . . lowest E-value is 1.6