View source for Results from GCAT undergraduates

<center><p><font size="6">Results from <b>G</b></font><font color="#ff0099" size="6"><b>C</b></font><font color="#00ba00" size="6"><b>A</b></font><font size="6"><b><font color="red">T</font></b> Undergraduates</font></p><hr></center>

<blockquote> 
    Here are some sample data from 
    Mary Lee Ledbetter's undergraduate lab at Holy Cross. These data were obtained 
    with the same RNA sample, so all spots &quot;should&quot; be equal (i.e. yellow). 
    Scans were conducted twice to obtain faint signals (long exposure) and to 
    prevent saturation of the CCD camera (short exposure). The RNA was isolated 
    from exponential phase cells grown aerobically on glucose. [http://www.bio.davidson.edu/projects/GCAT/GCAGprotocols.html#ISB ISB slide number was 2828]
    </blockquote>
<center>
{| border="1" cellpadding="2"
|-
! [http://www.bio.davidson.edu/projects/gcat/GCATprotocols.html#ISB <font size="5">'''Source'''</font>] !! <font color="#006600" size="4">Cy3 Tiff File</font> !! <font color="#990000" size="4">Cy5 Tiff File</font>
|-
! <font size="4">Long Exposure</font>
[[Image:MLL2828L.jpg|429px|]]
| [http://gcat.davidson.edu/gcat/data/MLL2828_L_w595.tif <font color="#006600" size="4">MLL2828_L_w595.tif</font>]||
[http://gcat.davidson.edu/gcat/data/MLL2828_L_w685.tif <font color="#990000" size="4">MLL2828_L_w685.tif</font>]
|-
! <font size="4">Short Exposure</font> 
[[Image:MLL2828_S.jpg|429px|]]
| [http://gcat.davidson.edu/gcat/data/MLL2828_L_w595.tif <font color="#006600" size="4">MLL2828_w595.tif</font>]||
[http://gcat.davidson.edu/gcat/data/MLL2828_L_w685.tif <font color="#990000" size="4">MLL2828_w685.tif</font>]
|-
|}</center>


== '''Davidson College, NC (Dr. A. Malcolm Campbell, faculty advisor)''' ==

*Danniel Choi's ('04) Bar Code Microarray - Pilot Study [[Media:Choi_2004.pps]] (1.6 MB PowerPoint Slide Show file) (Sedond place, 2004 North Carolina Academy of Sciences - collegiate division)

*Inexpensive DNA Microarrays and Analysis for Undergraduates [[Media:ASCB2003Postersmall.pdf]] (7 MB PDF file) (Presented at the 2003 Annual Meeting)

*Emilyh Oldham's ('03) Honors research on Microarray sources of variation [[Media:EOldham.ppt]] (500 kb power point file) (first place, too3 Horth Carolina Academy of Science - Collegiate division)

*Dan Pierce's ('03) research on improving and quantifying signal variation [[Media:DPierce.ppt]] (1.2 MB powerpoint file) (Third place, 2003 North Carolina Academy of Sciences - collegiate division)


== '''Pomona College, CA (Dr. Laura Hoopes, faculty advisor)''' ==

*12 students working in 5 groups examined DNA replication mutants v. wild-type

*[http://www.bio.davidson.edu/projects/gcat/SMDdirections.html Data available on Standord Mircoarray Database]

*[http://pages.pomona.edu/%7Ellh04747/genreg.html Genetic Regulation in Eukaryotes Course Page]


== '''Missouri Western State College, MO (Dr. Todd Eckdahl, Faculty Advisor)''' ==

*Five students examined effects of minor groove bindng agents

*[http://www.bio.davidson.edu/projects/gcat/SMDdirections.html Data available on Standord Mircoarray Database]

MWSC Student Presentations for 2000-2001 academic year (* denotes undergraduate authors):

# Hansen*, Edward L. "Using Microarray Technology to Measure Effects of Minor Groove Binding Drugs on Gene Expression in Yeast." Oral Presentation at the Missouri Academy of Science, Collegiate Division, April 20, 2001, Missouri Southern State College, 2nd place award.
# Eckdahl, Todd T, David Ash*, and Brianna Peterson*, "Using Microarray Technology in the Undergraduate Laboratory." Oral Presention at the Missouri Academy of Science, Senior Division, April 20, 2001, Missouri Southern State College.
# Brown*, Abby. "Single Gene and Genome Wide Expression Analysis of Glucose and Galactose Metabolism in Saccharomyces cerevisae." Poster Presentation at the Beta Beta Beta NC-2 District Convention, April 28, 2001, Reis Biological Station.
# Hansen*, Edward L. "Using Microarray Technology to Measure Effects of Minor Groove Binding Drugs on Gene Expression in Yeast." Oral Presentation at the Beta Beta Beta NC-2 District Convention, April 28, 2001, Reis Biological Station.


== '''Muhlenberg College, PA (Dr. Denise Wallack, faculty advisor)''' ==

*10 students examined the following conditions:

*(filled in soon)

*[http://www.bio.davidson.edu/projects/gcat/SMDdirections.html Data available on Standord Mircoarray Database]

*link to course page [http://www.muhlenberg.edu/depts/biology/courses/genome http://www.muhlenberg.edu/depts/biology/courses/genome]


== '''Swarthmore College, PA (Dr. Liz Vallen, faculty advisor)''' ==

*6 students examined DNA replication mutant V. wild-type

*[http://www.bio.davidson.edu/projects/gcat/SMDdirections.html Data available on Standord Mircoarray Database]

*link to research page [http://www.swarthmore.edu/NatSci/evallen1/projects.html http://www.swarthmore.edu/NatSci/evallen1/projects.html]


== '''Mount Saint Mary's College, MD (Dr. Myra Derbyshire, faculty advisor)''' ==

*8 students examined mutants that affect chromatin structure V. wild-type

*results presented at the Pennsylvania Academy of Science meeting in April, 2001

'''Microarray expression analysis of a ''Saccharomyces cerevisiae hst3 hst4'' double mutant'''

Dr. Myra K. Derbyshire<sup>1</sup>, Daniel J. Koppers<sup>1</sup>, Marlon G. Lawrence<sup>1</sup>, Heather L. Offutt<sup>1</sup>, Loretta M. Rathvon<sup>1</sup>, Harriete L. Sanderson<sup>1</sup>, Nabil A., Shafi1, Michael F. Smith1, Megan E. Walsh1, Dr. Jeffery Strathern<sup>2</sup>
<sup>1</sup>Genetics class. Mount Saint Mary's College, Emmitsburg, Maryland.
<sup>2</sup>NCI-Frederick Cancer Research and Development Center, Frederick, Maryland.

The ''Saccharomyces cerevisiae SIR2'' family of genes consists of SIR2 and four ''SIR2''-related genes (''HST1- HST4''). The ''SIR2'' gene product is a key regulator of gene expression believed to help form modified chromatin structures on the genes it regulates. ''SIR2'' is important for the structure of the nucleolus, for repressing gene expression near chromosome telomeres and at special sequences involved in cell-type differentiation. ''HST1-HST4'' gene products may modify chromatin structure at different chromosomal locations from those regulated by ''SIR2. S. cerevisiae hst3 hst4'' double mutants have low viability, defective telomeric silencing, and altered cell-cycle progression. A ''hst3::kanMX4'' (''Saccharomyces'' Deletion Research Project, #11801) was crossed to a ''hst4::kanMX4'' strain (#3350). The gene expression patterns of two meiotic segregants (''HST3 HST4, hst3 hst4'') were compared. Microarray technology was made possible through affiliation with '''GCAT''' (Genome Consortium for Active Teaching).


== '''California Lutheran University, CA (Dr. Denis Revie, faculty advisor)''' ==

*6 students examined anaerobic V. aerobic, and three concentrations of peroxide

*[http://www.bio.davidson.edu/projects/gcat/SMDdirections.html Data available on Standord Mircoarray Database]

*link to course page [http://public.clunet.edu/~revie/recombinant.htm http://public.clunet.edu/~revie/recombinant.htm]


== '''Lycoming College, PA (Dr. jeff Nerman, faculty advisor)''' ==

*8 students examined growth conditions and harsh growth conditions

*[http://www.bio.davidson.edu/projects/gcat/SMDdirections.html Data available on Standord Mircoarray Database]

*link to research page [http://srv2.lycoming.edu/~newman/research/research.html http://srv2.lycoming.edu/~newman/research/research.html]


== '''University of Southern Colorado (Dr. Dan Caprioglio, faculty advisor)''' ==

*students examined effects of drug treatments

*[http://www.bio.davidson.edu/projects/gcat/SMDdirections.html Data available on Standord Mircoarray Database]

*link to research page [http://csm.colostate-pueblo.edu/biology/dcaprio.html http://csm.colostate-pueblo.edu/biology/dcaprio.html]


== '''Carleton College, MN (Dr. Stephan Zweifel, faculty advisor)''' ==

*students examined effects of rich and minimum media

*[http://www.bio.davidson.edu/projects/gcat/SMDdirections.html Data available on Standord Mircoarray Database]

*link to faculty web page [http://www.acad.carleton.edu/curricular/BIOL/faculty/szweifel/index.html http://www.acad.carleton.edu/curricular/BIOL/faculty/szweifel/index.html]


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 <p><font size="5">G</font><font color="#ff0099" size="5">C</font><font color="#00ba00" size="5">A</font><font color="red" size="5">T</font><font color="black" size="5">members thank Drs. Pat Brown, David Botstein, Barbara Dunn, and the other members of the Stanford Microarray Database for their generosity in allowing undergraduate data to be included in their database.  We also thank Pat Brown and members of his lab for producing the yeast microarrays for our use. Without this support, </font><font size="5"><strong>G</strong></font><strong><font color="#ff0099" size="5">C</font><font color="#00ba00" size="5">A</font><font color="red" size="5">T</font></strong><font color="black" size="5"> would not have been so successful in its first year of existence.</font></p>


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<p><font size="4">&copy; Copyright 2004 Department of Biology, Davidson College, Davidson, NC 28035<br>
Send comments, questions, and suggestions to: [mailto:macampbell@davidson.edu <strong>macampbell@davidson.edu</strong>]</font>

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