Additional Ideas for Future Programmed Evolution Research - Revision history

Ekeffeler at 21:34, 11 June 2014

2014-06-11T21:34:20Z

Ekeffeler at 21:31, 11 June 2014

2014-06-11T21:31:16Z

Ekeffeler at 19:35, 11 June 2014

2014-06-11T19:35:40Z

Ekeffeler at 19:35, 11 June 2014

2014-06-11T19:35:16Z

Ekeffeler at 19:35, 11 June 2014

2014-06-11T19:35:00Z

Ekeffeler at 16:01, 11 June 2014

2014-06-11T16:01:19Z

Ekeffeler at 15:59, 11 June 2014

2014-06-11T15:59:14Z

Ekeffeler at 15:56, 11 June 2014

2014-06-11T15:56:47Z

Ekeffeler: Created page with '1. Develop a multistep pathway off of the caffeine->theophylline single step pathway (Find a substrate that creates caffeine)'

2014-06-11T15:47:06Z

Created page with '1. Develop a multistep pathway off of the caffeine->theophylline single step pathway (Find a substrate that creates caffeine)'

New page

1. Develop a multistep pathway off of the caffeine->theophylline single step pathway (Find a substrate that creates caffeine)

@@ Line 1: / Line 1: @@
 .	New System-
-            New Riboswitch, Multistep
+ New Riboswitch, Multistep
 -Ex: Adenosylcobalamin riboswitch-
@@ Line 11: / Line 11: @@
 .	Biosynthesis of Caffeine then Theophylline-
-           •Making our single-step pathway to a multi-step pathway
+ •Making our single-step pathway to a multi-step pathway
 .	Manipulating the promoter, RBS, origin, and chaperone-
-           •Ex: 5 promoters and 5 RBS’s or 500 promoters and 500 RBS’s
+ •Ex: 5 promoters and 5 RBS’s or 500 promoters and 500 RBS’s
 .	A. Measure clone distribution around different caffeine concentrations (disks)-
-           •Coloring the clock
+ •Coloring the clock
-        B. Measure clone distribution on different caffeine concentrations (plates)-
+.      B. Measure clone distribution on different caffeine concentrations (plates)-
-           •Ex: 2mM Caffeine -> clone #23 or 4mM caffeine-> clone ?
+ •Ex: 2mM Caffeine -> clone #23 or 4mM caffeine-> clone ?
-           •Cranking up selection
+ •Cranking up selection

@@ Line 1: / Line 1: @@
-. Develop a multistep pathway off of the caffeine->theophylline single step pathway (Find a substrate that creates caffeine)
+.	New System-
-. Thiamine pyrophosphate riboswitch [[File:Thiamine_Pyrophosphate_Riboswitch.pdf]]
+-Ex: Adenosylcobalamin riboswitch-
--In E.coli, thiamine monophosphate is converted to thiamine which then could be converted to thiamine pyrophosphate by thiamine kinase. For this particular riboswitch when thiamine pyrophosphate (TPP) binds to the riboswitch, it will stop transcription. A discussion about the thiamine pyrophosphate riboswitch resulted in the decision to not pursue this specific riboswitch because E.coli relies on this metabolic system to survive and it could be impossible to remove thiamine and still have them survive. This riboswitch is a great example of a multi-step metabolic pathway, but unfortunately it would be too detrimental to remove thiamine from E.coli’s system. It is still a goal to research a multi-step pathway as fascinating as the thiamine->>thiamine pyrophosphate, but still remain similar and practical to the caffeine->theophylline system.
+-Ex:Thiamine pyrophosphate riboswitch [[File:Thiamine_Pyrophosphate_Riboswitch.pdf]]

@@ Line 2: / Line 2: @@
 . Thiamine pyrophosphate riboswitch [[File:Thiamine_Pyrophosphate_Riboswitch.pdf]]
-−
-   -In E.coli, thiamine monophosphate is converted to thiamine which then could be converted to thiamine pyrophosphate by thiamine kinase. For this particular riboswitch when thiamine pyrophosphate (TPP) binds to the riboswitch, it will stop transcription. A discussion about the thiamine pyrophosphate riboswitch resulted in the decision to not pursue this specific riboswitch because E.coli relies on this metabolic system to survive and it could be impossible to remove thiamine and still have them survive. This riboswitch is a great example of a multi-step metabolic pathway, but unfortunately it would be too detrimental to remove thiamine from E.coli’s system. It is still a goal to research a multi-step pathway as fascinating as the thiamine->>thiamine pyrophosphate, but still remain similar and practical to the caffeine->theophylline system.
+-In E.coli, thiamine monophosphate is converted to thiamine which then could be converted to thiamine pyrophosphate by thiamine kinase. For this particular riboswitch when thiamine pyrophosphate (TPP) binds to the riboswitch, it will stop transcription. A discussion about the thiamine pyrophosphate riboswitch resulted in the decision to not pursue this specific riboswitch because E.coli relies on this metabolic system to survive and it could be impossible to remove thiamine and still have them survive. This riboswitch is a great example of a multi-step metabolic pathway, but unfortunately it would be too detrimental to remove thiamine from E.coli’s system. It is still a goal to research a multi-step pathway as fascinating as the thiamine->>thiamine pyrophosphate, but still remain similar and practical to the caffeine->theophylline system.

← Older revision		Revision as of 19:35, 11 June 2014
Line 3:		Line 3:
	2. Thiamine pyrophosphate riboswitch [[File:Thiamine_Pyrophosphate_Riboswitch.pdf]]		2. Thiamine pyrophosphate riboswitch [[File:Thiamine_Pyrophosphate_Riboswitch.pdf]]

−	--In E.coli, thiamine monophosphate is converted to thiamine which then could be converted to thiamine pyrophosphate by thiamine kinase. For this particular riboswitch when thiamine pyrophosphate (TPP) binds to the riboswitch, it will stop transcription. A discussion about the thiamine pyrophosphate riboswitch resulted in the decision to not pursue this specific riboswitch because E.coli relies on this metabolic system to survive and it could be impossible to remove thiamine and still have them survive. This riboswitch is a great example of a multi-step metabolic pathway, but unfortunately it would be too detrimental to remove thiamine from E.coli’s system. It is still a goal to research a multi-step pathway as fascinating as the thiamine->>thiamine pyrophosphate, but still remain similar and practical to the caffeine->theophylline system.	+	-In E.coli, thiamine monophosphate is converted to thiamine which then could be converted to thiamine pyrophosphate by thiamine kinase. For this particular riboswitch when thiamine pyrophosphate (TPP) binds to the riboswitch, it will stop transcription. A discussion about the thiamine pyrophosphate riboswitch resulted in the decision to not pursue this specific riboswitch because E.coli relies on this metabolic system to survive and it could be impossible to remove thiamine and still have them survive. This riboswitch is a great example of a multi-step metabolic pathway, but unfortunately it would be too detrimental to remove thiamine from E.coli’s system. It is still a goal to research a multi-step pathway as fascinating as the thiamine->>thiamine pyrophosphate, but still remain similar and practical to the caffeine->theophylline system.

← Older revision		Revision as of 19:35, 11 June 2014
Line 1:		Line 1:
	1. Develop a multistep pathway off of the caffeine->theophylline single step pathway (Find a substrate that creates caffeine)		1. Develop a multistep pathway off of the caffeine->theophylline single step pathway (Find a substrate that creates caffeine)

−	2. Thiamine riboswitch [[File:Thiamine_Pyrophosphate_Riboswitch.pdf]]	+	2. Thiamine pyrophosphate riboswitch [[File:Thiamine_Pyrophosphate_Riboswitch.pdf]]
		+
		+	--In E.coli, thiamine monophosphate is converted to thiamine which then could be converted to thiamine pyrophosphate by thiamine kinase. For this particular riboswitch when thiamine pyrophosphate (TPP) binds to the riboswitch, it will stop transcription. A discussion about the thiamine pyrophosphate riboswitch resulted in the decision to not pursue this specific riboswitch because E.coli relies on this metabolic system to survive and it could be impossible to remove thiamine and still have them survive. This riboswitch is a great example of a multi-step metabolic pathway, but unfortunately it would be too detrimental to remove thiamine from E.coli’s system. It is still a goal to research a multi-step pathway as fascinating as the thiamine->>thiamine pyrophosphate, but still remain similar and practical to the caffeine->theophylline system.

← Older revision		Revision as of 16:01, 11 June 2014
Line 1:		Line 1:
	1. Develop a multistep pathway off of the caffeine->theophylline single step pathway (Find a substrate that creates caffeine)		1. Develop a multistep pathway off of the caffeine->theophylline single step pathway (Find a substrate that creates caffeine)

−	2. Thiamine riboswitch [[Thiamine_Pyrophosphate_Riboswitch.pdf]]	+	2. Thiamine riboswitch [[File:Thiamine_Pyrophosphate_Riboswitch.pdf]]

← Older revision		Revision as of 15:59, 11 June 2014
Line 1:		Line 1:
	1. Develop a multistep pathway off of the caffeine->theophylline single step pathway (Find a substrate that creates caffeine)		1. Develop a multistep pathway off of the caffeine->theophylline single step pathway (Find a substrate that creates caffeine)

−	2. Thiamine riboswitch	+	2. Thiamine riboswitch [[Thiamine_Pyrophosphate_Riboswitch.pdf]]

← Older revision		Revision as of 15:56, 11 June 2014
Line 1:		Line 1:
	1. Develop a multistep pathway off of the caffeine->theophylline single step pathway (Find a substrate that creates caffeine)		1. Develop a multistep pathway off of the caffeine->theophylline single step pathway (Find a substrate that creates caffeine)
		+
		+	2. Thiamine riboswitch