https://gcat.davidson.edu/GcatWiki/index.php?action=history&feed=atom&title=Cellular_Signaling_Systems 2026-04-23T00:55:08Z Revision history for this page on the wiki MediaWiki 1.28.2 https://gcat.davidson.edu/GcatWiki/index.php?title=Cellular_Signaling_Systems&diff=6958&oldid=prev 2008-10-28T14:42:59Z

<p></p> <p><b>New page</b></p><div>== Las/Rhl cell signaling system ==<br /> '''Responsible''': Robert Cool, Alicia Allen, and Erin Feeney<br /> <br /> '''Las System'''<br /> <br /> '''Signal Molecule''': An AHL called PAI-1 (N-3-oxododecanoyl-l-hsl)(3-oxo-C12-hsl)<br /> <br /> '''Bacterial species''': Pseudomonas aeruginosa gram(-) possibly E.coli (see article 3)<br /> <br /> '''Receiver protein''': LasR<br /> <br /> '''Effect of binding''': TXN activation of virulence genes, lasA, lasB, apr, toxR<br /> <br /> '''Synthase''': LasI enzyme<br /> <br /> '''Target Genes''': lasI, lasA, lasB, apr, toxR<br /> <br /> '''Regulation''': unknown<br /> <br /> <br /> '''References'''<br /> <br /> &quot;Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes&quot; <br /> <br /> JP Pearson, EC Pesci and BH Iglewski <br /> [http://jb.asm.org/cgi/reprint/179/18/5756?maxtoshow=&amp;HITS=10&amp;hits=10&amp;RESULTFORMAT=&amp;titleabstract=las&amp;searchid=1&amp;FIRSTINDEX=0&amp;resourcetype=HWCIT]<br /> <br /> <br /> &quot;Regulation of Pseudomonas Quinolone Signal Synthesis in Pseudomonas aeruginosa&quot;<br /> <br /> Dana S. Wade, M. Worth Calfee, Edson R. Rocha, Elizabeth A. Ling, Elana Engstrom, James P. Coleman, and Everett C. Pesci<br /> [http://jb.asm.org/cgi/content/full/187/13/4372?view=long&amp;pmid=15968046 2]<br /> <br /> <br /> Posttranscriptional Control of Quorum-Sensing-Dependent Virulence Genes by DksA in Pseudomonas aeruginosa<br /> <br /> Florence Jude,Thilo Köhler,Pavel Branny,Karl Perron,Matthias P. Mayer,Rachel Comte, and Christian van Delden<br /> [http://jb.asm.org/cgi/content/full/185/12/3558?view=long&amp;pmid=12775693 3]<br /> <br /> Pending: [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1447470]<br /> <br /> '''Rhl System''' <br /> <br /> &lt;br&gt;<br /> '''Signal Molecule''': An AHL called PAI-2, Plasminogen activator inhibitor-2, N-butanoyl-homoserine lactone (C4HSL) <br /> <br /> '''Bacterial species''': Pseudomonas aeruginosa, gram(-)<br /> <br /> '''Receiver Protein''': Rhl R <br /> <br /> '''Effect of Binding''': activation of Rhamnosyl Transferase, then making RL (rhamnolipid) <br /> <br /> '''Synthase''': RhlA and RhlB <br /> <br /> '''Target Genes''': pqsABCDE and phnAB<br /> <br /> '''Regulation''': unknown<br /> <br /> '''References'''<br /> <br /> [http://jb.asm.org/cgi/reprint/189/13/4827 background information on Las and Rhl]<br /> <br /> [[Image:Las_rhl.gif]]<br /> <br /> '''Parts Needed''':<br /> <br /> LasR + pro/term<br /> <br /> RhlR + pro/term<br /> <br /> RhlI + pro/term<br /> <br /> pqsABCDE + pro/term<br /> <br /> pqsR<br /> <br /> pqsH + pro/term<br /> <br /> phnAB<br /> <br /> LasI<br /> <br /> RBS<br /> <br /> == Lux cell signaling system ==<br /> <br /> '''Responsible:''' Andrew Gordon and Pallavi Penumetcha<br /> <br /> '''Signal molecule:''' ''N''-acyl-homoserine lactone (AHL) Generic term for a variety of species specific hormone-like molecules <br /> <br /> '''Bacterial species:''' discovered in ''Vibrio fischeri'' known to work in ''E. coli''<br /> <br /> '''Receiver protein:''' LuxR protein receives signal from AHL; also has some control over transciption of luciferase<br /> <br /> '''Signal molecule synthase:''' LuxI; also has some control over transciption of luciferase<br /> <br /> '''Additional Information:''' &quot;Quorum Quenching&quot; aiiA (intracellular) lactonase reduces AHL concentration<br /> <br /> [[Image:800px-Luxrreceiverschematic.png]]<br /> <br /> '''Resources'''<br /> <br /> [http://partsregistry.org/Lux Lux Operon Pathway]<br /> <br /> [http://partsregistry.org/AHL AHL signaling molecules by species; some are specific to gram pos but may affect gram negs]<br /> <br /> [http://www.horizonpress.com/jmmb/v1/v1n1/03.pdf Reducing Crosstalk in Lux System]<br /> <br /> '''References'''<br /> <br /> [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=522112 Quorum Quenching to control Lux Pathway]<br /> <br /> [http://www.nature.com/nature/journal/v434/n7037/full/nature03461.html] A Synthetic multicellular system for programmed pattern formation<br /> <br /> '''Parts Needed''':<br /> <br /> LuxR + pro/term<br /> <br /> RBS<br /> <br /> LuxI + pro/term<br /> <br /> LuxI sender</div>

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