https://gcat.davidson.edu/GcatWiki/index.php?action=history&feed=atom&title=Control_of_Gene_Expression_to_Fit_ModelControl of Gene Expression to Fit Model - Revision history2026-05-20T18:35:33ZRevision history for this page on the wikiMediaWiki 1.28.2https://gcat.davidson.edu/GcatWiki/index.php?title=Control_of_Gene_Expression_to_Fit_Model&diff=1393&oldid=prevWikiSysop at 16:18, 27 June 20072007-06-27T16:18:30Z<p></p>
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<td colspan='2' style="background-color: white; color:black; text-align: center;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black; text-align: center;">Revision as of 16:18, 27 June 2007</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>[[pLac]]</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>[[pLac]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div><br></div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div><br></div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>The Lac operon promoter (pLac) was an attractive first option due to its ability to transcribe multiple adjacent genes in the Lac operon (lacZ, lacY, and lacA are all enzymes used for sugar metabolism) a controllable mechanism of repression.</div></td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>The Lac operon promoter (pLac) was an attractive first option due to its ability to transcribe multiple adjacent genes in the Lac operon (lacZ, lacY, and lacA are all enzymes used for sugar metabolism) a controllable mechanism of repression<ins class="diffchange diffchange-inline">(lacI)</ins>.</div></td></tr>
</table>WikiSysophttps://gcat.davidson.edu/GcatWiki/index.php?title=Control_of_Gene_Expression_to_Fit_Model&diff=1392&oldid=prevWikiSysop at 16:17, 27 June 20072007-06-27T16:17:58Z<p></p>
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<td colspan='2' style="background-color: white; color:black; text-align: center;">Revision as of 16:17, 27 June 2007</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>Successful biological modeling of the HPP requires us to be able to transcribe multiple adjacent genes. Due to the nature of our "flippable" hix-hin recombinase model, insertion of promoter sequence after the start of transcription is not practical. Our team needs a strong promoter to be able to sucessfully transcribe all the subsequent genes without the help of a second promoter downstream.</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>Successful biological modeling of the HPP requires us to be able to transcribe multiple adjacent genes. Due to the nature of our "flippable" hix-hin recombinase model, insertion of promoter sequence after the start of transcription is not practical. Our team needs a strong promoter to be able to sucessfully transcribe all the subsequent genes without the help of a second promoter downstream.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Using the biobrick system we were able to choose any promoter from any operon in the E.Coli genome. </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>[[pLac]]</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>[[pLac]]</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del class="diffchange diffchange-inline">Using the biobrick system we were able to choose any promoter from any operon in the E.Coli genome. </del>The Lac operon promoter (pLac) was an attractive first option due to its ability to transcribe multiple adjacent genes in the Lac operon (lacZ, lacY, and lacA are all enzymes used for sugar metabolism) a controllable mechanism of repression.</div></td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>The Lac operon promoter (pLac) was an attractive first option due to its ability to transcribe multiple adjacent genes in the Lac operon (lacZ, lacY, and lacA are all enzymes used for sugar metabolism) a controllable mechanism of repression.</div></td></tr>
</table>WikiSysophttps://gcat.davidson.edu/GcatWiki/index.php?title=Control_of_Gene_Expression_to_Fit_Model&diff=1391&oldid=prevWikiSysop at 16:16, 27 June 20072007-06-27T16:16:40Z<p></p>
<p><b>New page</b></p><div>==[[Promotion]]==<br />
<br />
Successful biological modeling of the HPP requires us to be able to transcribe multiple adjacent genes. Due to the nature of our "flippable" hix-hin recombinase model, insertion of promoter sequence after the start of transcription is not practical. Our team needs a strong promoter to be able to sucessfully transcribe all the subsequent genes without the help of a second promoter downstream.<br />
<br />
[[pLac]]<br />
Using the biobrick system we were able to choose any promoter from any operon in the E.Coli genome. The Lac operon promoter (pLac) was an attractive first option due to its ability to transcribe multiple adjacent genes in the Lac operon (lacZ, lacY, and lacA are all enzymes used for sugar metabolism) a controllable mechanism of repression.</div>WikiSysop