DM Notes 1.26.16 - Revision history

Dymaghini at 19:55, 26 January 2016

2016-01-26T19:55:10Z

Dymaghini at 19:54, 26 January 2016

2016-01-26T19:54:47Z

Dymaghini at 19:54, 26 January 2016

2016-01-26T19:54:17Z

Dymaghini at 19:52, 26 January 2016

2016-01-26T19:52:54Z

Dymaghini: Created page with "Today, we downloaded the trimmed reads (no barcode at the start of each read). Apparent differences: per sequence tile quality decreased, GC content improved, sequence length ..."

2016-01-26T19:48:37Z

Created page with "Today, we downloaded the trimmed reads (no barcode at the start of each read). Apparent differences: per sequence tile quality decreased, GC content improved, sequence length ..."

New page

Today, we downloaded the trimmed reads (no barcode at the start of each read). Apparent differences: per sequence tile quality decreased, GC content improved, sequence length distribution decreased- more variation in length).
For sequence length distribution: barcodes were trimmed. Reads with low phred scores were trimmed from the 3' end, which explains why there are some odd length sequences. However, if the sequences got to fewer than 30 nucleotides, they got tossed.

← Older revision		Revision as of 19:55, 26 January 2016
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	Apparent differences: per sequence tile quality decreased, GC content improved, sequence length distribution decreased- more variation in length).		Apparent differences: per sequence tile quality decreased, GC content improved, sequence length distribution decreased- more variation in length).
	For sequence length distribution: barcodes were trimmed. Reads with low phred scores were trimmed from the 3' end, which explains why there are some odd length sequences. However, if the sequences got to fewer than 30 nucleotides, they got tossed.		For sequence length distribution: barcodes were trimmed. Reads with low phred scores were trimmed from the 3' end, which explains why there are some odd length sequences. However, if the sequences got to fewer than 30 nucleotides, they got tossed.
−	~~[http://www.davidson.edu link title]~~

@@ Line 1: / Line 1: @@
-[file:///Users/dymaghini/Desktop/Bio343/fastQC/fastQCintestine/split_1no_i_fastqc.html Split 1]Today, we downloaded the trimmed reads (no barcode at the start of each read). The file Tues_19 Jan_2016.docx has instructions.
+Today, we downloaded the trimmed reads (no barcode at the start of each read). The file Tues_19 Jan_2016.docx has instructions.
 Apparent differences: per sequence tile quality decreased, GC content improved, sequence length distribution decreased- more variation in length).
 For sequence length distribution: barcodes were trimmed. Reads with low phred scores were trimmed from the 3' end, which explains why there are some odd length sequences. However, if the sequences got to fewer than 30 nucleotides, they got tossed.

@@ Line 1: / Line 1: @@
-Today, we downloaded the trimmed reads (no barcode at the start of each read). The file Tues_19 Jan_2016.docx has instructions.
+[file:///Users/dymaghini/Desktop/Bio343/fastQC/fastQCintestine/split_1no_i_fastqc.html Split 1]Today, we downloaded the trimmed reads (no barcode at the start of each read). The file Tues_19 Jan_2016.docx has instructions.
 Apparent differences: per sequence tile quality decreased, GC content improved, sequence length distribution decreased- more variation in length).
 For sequence length distribution: barcodes were trimmed. Reads with low phred scores were trimmed from the 3' end, which explains why there are some odd length sequences. However, if the sequences got to fewer than 30 nucleotides, they got tossed.

← Older revision		Revision as of 19:52, 26 January 2016
Line 1:		Line 1:
−	Today, we downloaded the trimmed reads (no barcode at the start of each read). Apparent differences: per sequence tile quality decreased, GC content improved, sequence length distribution decreased- more variation in length).	+	Today, we downloaded the trimmed reads (no barcode at the start of each read). The file Tues_19 Jan_2016.docx has instructions.
		+
		+	Apparent differences: per sequence tile quality decreased, GC content improved, sequence length distribution decreased- more variation in length).
	For sequence length distribution: barcodes were trimmed. Reads with low phred scores were trimmed from the 3' end, which explains why there are some odd length sequences. However, if the sequences got to fewer than 30 nucleotides, they got tossed.		For sequence length distribution: barcodes were trimmed. Reads with low phred scores were trimmed from the 3' end, which explains why there are some odd length sequences. However, if the sequences got to fewer than 30 nucleotides, they got tossed.