Davidson Missouri W/Gene splitting - Revision history

Amshoecraft at 15:18, 9 August 2007

2007-08-09T15:18:25Z

WikiSysop: /* The Genes */

2007-07-13T16:38:49Z

‎The Genes

Synthetic Students at 19:53, 11 July 2007

2007-07-11T19:53:10Z

Synthetic Students at 19:52, 11 July 2007

2007-07-11T19:52:20Z

Synthetic Students at 19:52, 11 July 2007

2007-07-11T19:52:03Z

Synthetic Students at 19:50, 11 July 2007

2007-07-11T19:50:51Z

Synthetic Students at 19:50, 11 July 2007

2007-07-11T19:50:33Z

Synthetic Students at 19:41, 11 July 2007

2007-07-11T19:41:39Z

Synthetic Students: Gene splitting moved to Davidson Missouri W/Gene splitting

2007-07-11T19:36:10Z

Gene splitting moved to Davidson Missouri W/Gene splitting

Synthetic Students at 19:29, 11 July 2007

2007-07-11T19:29:19Z

← Older revision		Revision as of 15:18, 9 August 2007
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−	<center>[[Davidson Missouri W\| <span style="color:red">Home</span>]] \| [[Davidson Missouri W/Background Information\| <span style="color:red">Background Information</span>]] \| [[Davidson Missouri W/Solving the HPP in vivo\| <span style="color:red">Current Project: Solving the Hamiltonian Path Problem ''in vivo''</span>]] \| [[Davidson Missouri W/~~Probability and Statistics~~\| <span style="color:red">~~Probability and Statistics~~</span>]] \| [[Davidson Missouri W/Gene splitting\| <span style="color:black"> Gene Splitting </span>]] \| [[Davidson Missouri W/Controlling Expression\| <span style="color:red"> Controlling Expression </span>]] \| [[Davidson Missouri W/Traveling Salesperson Problem\| <span style="color:red">Traveling Salesperson Problem</span> ]] \| [[Davidson Missouri W/Backwards promotion and read-through transcription\| <span style="color:red">Backwards Promotion and Read-Through Transcription</span>]] \| [[Davidson Missouri W/Resources and Citations\|<span style="color:red">Resources and Citations</span>]]</center>	+	<center>[[Davidson Missouri W\| <span style="color:red">Home</span>]] \| [[Davidson Missouri W/Background Information\| <span style="color:red">Background Information</span>]] \| [[Davidson Missouri W/Solving the HPP in vivo\| <span style="color:red">Current Project: Solving the Hamiltonian Path Problem ''in vivo''</span>]] \| [[Davidson Missouri W/Mathematical Modeling\| <span style="color:red">Mathematical Modeling</span>]] \| [[Davidson Missouri W/Gene splitting\| <span style="color:black"> Gene Splitting </span>]] \| [[Davidson Missouri W/Controlling Expression\| <span style="color:red"> Controlling Expression </span>]] \| [[Davidson Missouri W/Traveling Salesperson Problem\| <span style="color:red">Traveling Salesperson Problem</span> ]] \| [[Davidson Missouri W/Backwards promotion and read-through transcription\| <span style="color:red">Backwards Promotion and Read-Through Transcription</span>]] \| [[Davidson Missouri W/Resources and Citations\|<span style="color:red">Resources and Citations</span>]]</center>
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 [http://www.ingentaconnect.com/content/els/00452068/1999/00000027/00000005/art91144 1]
-Our goal was to insert a hix site (a polar molecule) in an area of KNTase protein that would not interfere with its ability to inhibit Kanamycin. We looked at mutational analysis of KNTase and other aminoglycoside phosphotransferase enzymes to determine which aspects of KNTase’s structure were integral to its function and therefore not an ideal site for hix site insertion. KNTase is a dimmer consisting of 253 amino acids in the molecule [http://www.bioscience.org/1999/v4/d/wright/fulltext.htm 3]. In looking at conserved structures in the APH family we took into consideration that:
+Our goal was to insert a hix site (a polar molecule) in an area of KNTase protein that would not interfere with its ability to inhibit Kanamycin. We looked at mutational analysis of KNTase and other aminoglycoside phosphotransferase enzymes to determine which aspects of KNTase’s structure were integral to its function and therefore not an ideal site for hix site insertion. KNTase is a dimer consisting of 253 amino acids in the molecule [http://www.bioscience.org/1999/v4/d/wright/fulltext.htm 3]. In looking at conserved structures in the APH family we took into consideration that:
 -Substitution of AA 190 caused 650-fold decrease in enzyme activity [http://www.bioscience.org/1999/v4/d/perlin/fulltext.htm 2]

@@ Line 2: / Line 2: @@
 <hr>
-We have selected 4 genes to split. We will use our [http://gcat.davidson.edu/iGEM07/genesplitter.html online] gene splitting [[Davidson Missouri W/web tool|web tool]] to choose the PCR primers. Davidson will produce these 4 split genes and test each one.
+We have selected 4 genes to split. We will use our [http://gcat.davidson.edu/iGEM07/genesplitter.html online] gene splitting [[Davidson Missouri W/Web tool| web tool]] to choose the PCR primers. Davidson will produce these 4 split genes and test each one.
 # [[Davidson Missouri W/Gene splitting#Kanamycin|Kanamycin Nucleotidyltransferase]]

@@ Line 2: / Line 2: @@
 <hr>
-We have selected 4 genes to split. We will use our [http://gcat.davidson.edu/iGEM07/genesplitter.html online] gene splitting [[Davidson Missouri W/Web Tool|web tool]] to choose the PCR primers. Davidson will produce these 4 split genes and test each one.
+We have selected 4 genes to split. We will use our [http://gcat.davidson.edu/iGEM07/genesplitter.html online] gene splitting [[Davidson Missouri W/web tool|web tool]] to choose the PCR primers. Davidson will produce these 4 split genes and test each one.
 # [[Davidson Missouri W/Gene splitting#Kanamycin|Kanamycin Nucleotidyltransferase]]

@@ Line 2: / Line 2: @@
 <hr>
-We have selected 4 genes to split. We will use our [http://gcat.davidson.edu/iGEM07/genesplitter.html online] gene splitting [[web tool]] to choose the PCR primers. Davidson will produce these 4 split genes and test each one.
+We have selected 4 genes to split. We will use our [http://gcat.davidson.edu/iGEM07/genesplitter.html online] gene splitting [[Davidson Missouri W/Web Tool|web tool]] to choose the PCR primers. Davidson will produce these 4 split genes and test each one.
 # [[Davidson Missouri W/Gene splitting#Kanamycin|Kanamycin Nucleotidyltransferase]]

@@ Line 9: / Line 9: @@
 # [[Davidson Missouri W/Gene splitting#Cre|Cre Recombinase]]
 <br>
-[[Davidson Missouri W/|Return to DMW main page]]
+[[Davidson Missouri W|Return to DMW main page]]
 <br>
 =The Genes=

@@ Line 4: / Line 4: @@
 We have selected 4 genes to split. We will use our [http://gcat.davidson.edu/iGEM07/genesplitter.html online] gene splitting [[web tool]] to choose the PCR primers. Davidson will produce these 4 split genes and test each one.
-# [[Gene splitting#Kanamycin|Kanamycin Nucleotidyltransferase]]
+# [[Davidson Missouri W/Gene splitting#Kanamycin|Kanamycin Nucleotidyltransferase]]
-# [[Gene splitting#RFP|Red Fluorescent Protein]]
+# [[Davidson Missouri W/Gene splitting#RFP|Red Fluorescent Protein]]
-# [[Gene splitting#CAT|Chloramphenicol Acetyltransferase]]
+# [[Davidson Missouri W/Gene splitting#CAT|Chloramphenicol Acetyltransferase]]
-# [[Gene splitting#Cre|Cre Recombinase]]
+# [[Davidson Missouri W/Gene splitting#Cre|Cre Recombinase]]
 <br>
-[[Davidson/Missouri Western iGEM 2007|Return to DMW main page]]
+[[Davidson Missouri W/|Return to DMW main page]]
 <br>
 =The Genes=

← Older revision		Revision as of 19:41, 11 July 2007
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−	<center>[[Davidson/Missouri W\| <span style="color:red">Home</span>]] \| [[Background Information\| <span style="color:red">Background Information</span>]] \| [[Solving the HPP in vivo\| <span style="color:red">Current Project: Solving the Hamiltonian Path Problem ''in vivo''</span>]] \| [[Probability and Statistics\| <span style="color:red">Probability and Statistics</span>]] \| [[Gene splitting\| <span style="color:black"> Gene Splitting </span>]] \| [[Controlling Expression\| <span style="color:red"> Controlling Expression </span>]] \| [[Traveling Salesperson Problem\| <span style="color:red">Traveling Salesperson Problem</span> ]] \| [[Backwards promotion and read-through transcription\| <span style="color:red">Backwards ~~promotion~~ and Read-Through Transcription</span>]] \| [[Resources and Citations\|<span style="color:red">Resources and Citations</span>]]</center>	+	<center>[[Davidson Missouri W\| <span style="color:red">Home</span>]] \| [[Davidson Missouri W/Background Information\| <span style="color:red">Background Information</span>]] \| [[Davidson Missouri W/Solving the HPP in vivo\| <span style="color:red">Current Project: Solving the Hamiltonian Path Problem ''in vivo''</span>]] \| [[Davidson Missouri W/Probability and Statistics\| <span style="color:red">Probability and Statistics</span>]] \| [[Davidson Missouri W/Gene splitting\| <span style="color:black"> Gene Splitting </span>]] \| [[Davidson Missouri W/Controlling Expression\| <span style="color:red"> Controlling Expression </span>]] \| [[Davidson Missouri W/Traveling Salesperson Problem\| <span style="color:red">Traveling Salesperson Problem</span> ]] \| [[Davidson Missouri W/Backwards promotion and read-through transcription\| <span style="color:red">Backwards Promotion and Read-Through Transcription</span>]] \| [[Davidson Missouri W/Resources and Citations\|<span style="color:red">Resources and Citations</span>]]</center>
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← Older revision	Revision as of 19:36, 11 July 2007
(No difference)

← Older revision		Revision as of 19:29, 11 July 2007
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−	<center>[[Davidson/Missouri ~~Western iGEM 2007~~\| <span style="color:red">Home</span>]] \| [[Background Information\| <span style="color:red">Background Information</span>]] \| [[Solving the HPP in vivo\| <span style="color:red">Current Project: Solving the Hamiltonian Path Problem ''in vivo''</span>]] \| [[Probability and Statistics\| <span style="color:red">Probability and Statistics</span>]] \| [[Gene splitting\| <span style="color:black"> Gene Splitting </span>]] \| [[Controlling Expression\| <span style="color:red"> Controlling Expression </span>]] \| [[Traveling Salesperson Problem\| <span style="color:red">Traveling Salesperson Problem</span> ]] \| [[Backwards promotion and read-through transcription\| <span style="color:red">Backwards promotion and Read-Through Transcription</span>]] \| [[Resources and Citations\|<span style="color:red">Resources and Citations</span>]]</center>	+	<center>[[Davidson/Missouri W\| <span style="color:red">Home</span>]] \| [[Background Information\| <span style="color:red">Background Information</span>]] \| [[Solving the HPP in vivo\| <span style="color:red">Current Project: Solving the Hamiltonian Path Problem ''in vivo''</span>]] \| [[Probability and Statistics\| <span style="color:red">Probability and Statistics</span>]] \| [[Gene splitting\| <span style="color:black"> Gene Splitting </span>]] \| [[Controlling Expression\| <span style="color:red"> Controlling Expression </span>]] \| [[Traveling Salesperson Problem\| <span style="color:red">Traveling Salesperson Problem</span> ]] \| [[Backwards promotion and read-through transcription\| <span style="color:red">Backwards promotion and Read-Through Transcription</span>]] \| [[Resources and Citations\|<span style="color:red">Resources and Citations</span>]]</center>

	<hr>		<hr>