Experience gained on new Registry parts - Revision history

Eckdahl at 01:00, 28 October 2008

2008-10-28T01:00:31Z

2008-10-28T00:56:26Z

2008-10-28T00:55:08Z

2008-10-28T00:51:58Z

2008-10-28T00:51:16Z

2008-10-28T00:50:02Z

2008-10-28T00:46:22Z

2008-10-28T00:42:29Z

2008-10-28T00:42:07Z

2008-10-28T00:36:21Z

← Older revision		Revision as of 01:00, 28 October 2008
Line 18:		Line 18:

	<br>		<br>
−
−	'''Improved function by a modified Lac promoter''' - Mutations were introduced into the wild type Lac promoter that exists in the Registry. One mutant promoter, designated pLacIQ1, had improved ability to promote, as demonstrated by the following experiment.
−
−	~~<br>~~
−
−	Constructs were assembled with eiter the wild type Lac promoter or mutant promoters driving GFP expression via part number E0240. Fluorescence measurements taken at 16 or 24 hours were corrected for optical density.
−
−	~~16 hours~~
−	~~{\| border="1" cellpadding="2"~~
−	~~!width="30"\|Sample~~
−	~~!width="30"\|Fluorescence~~
−	~~!width="30"\|Optical Density~~
−	~~!width="30"\|Fluor/OD~~
−	\|-
−	~~\|LB \|\| 2\|\| 0.043 \|\|46.5\|\|~~
−	\|-
−	~~\|E0240\|\| 0.67 \|\|1.262 \|\|0.53\|\|~~
−	\|-
−	~~\|S04020 \|\|4.67 \|\|1.225\|\| 3.81\|\|~~
−	\|-
−	~~\|S04021 \|\|21 \|\|1.201\|\| 17.49\|\|~~
−	\|-
−	~~\|K091131\|\| 68\|\| 1.188 \|\|57.24\|\|~~
−	\|-
−	\|}
−
−	~~24 hours~~
−
−	~~{\| border="1" cellpadding="2"~~
−	~~!width="30"\|Sample~~
−	~~!width="30"\|Fluorescence~~
−	~~!width="30"\|Optical Density~~
−	~~!width="30"\|Fluor/OD~~
−	\|-
−	~~\|LB \|\| 1\|\| 0.047 \|\|21.3\|\|~~
−	\|-
−	~~\|E0240\|\| 3.0 \|\|1.201 \|\|2.50\|\|~~
−	\|-
−	~~\|S04020 \|\|4.0 \|\|1.239\|\| 3.23\|\|~~
−	\|-
−	~~\|S04021 \|\|14.5 \|\|1.216\|\| 11.92\|\|~~
−	\|-
−	~~\|K091131\|\| 71.3\|\| 1.207 \|\|59.07\|\|~~
−	\|-
−	\|}
−
−	~~[[Image:16_hr.JPG\|389px]]~~
−
−	~~[[Image:24_hr.JPG\|389px]]~~

@@ Line 19: / Line 19: @@
 <br>
-'''Improved function by a modified Lac promoter''' - Mutations were introduced into the wild type Lac promoter that exists in the Registry.  One mutant promoter, designated pLacIQ1, had improved ability to promote, as demostrated by the following experiment.
+'''Improved function by a modified Lac promoter''' - Mutations were introduced into the wild type Lac promoter that exists in the Registry.  One mutant promoter, designated pLacIQ1, had improved ability to promote, as demonstrated by the following experiment.
 <br>
@@ Line 64: / Line 64: @@
 |}
 [[Image:16_hr.JPG|389px]]
 [[Image:24_hr.JPG|389px]]

@@ Line 44: / Line 44: @@
 |}
-hr.
+hours
 Sample	Flourescence	OD	Flourescence/OD
 LB + Amp	1	0.047	21.27659574

@@ Line 29: / Line 29: @@
 !width="30"|Sample
 !width="30"|Fluorescence
-!width="300"|Optical Density
+!width="30"|Optical Density
 !width="30"|Fluor/OD
 |-

@@ Line 32: / Line 32: @@
 !width="30"|Fluor/OD
 |-
-|LB ||	2||	0.043	||46.5|-
+|LB ||	2||	0.043	||46.5||
-|E0240||	0.67	||1.262	||0.53|-
+|-
-|S04020	||4.67	||1.225||	3.81|-
+|E0240||	0.67	||1.262	||0.53||
-|S04021	||21	||1.201||	17.49|-
+|-
-|K091131||	68||	1.188	||57.24
+|S04020	||4.67	||1.225||	3.81||
 |-
 |}

← Older revision		Revision as of 00:46, 28 October 2008
Line 24:		Line 24:

	Constructs were assembled with eiter the wild type Lac promoter or mutant promoters driving GFP expression via part number E0240. Fluorescence measurements taken at 16 or 24 hours were corrected for optical density.		Constructs were assembled with eiter the wild type Lac promoter or mutant promoters driving GFP expression via part number E0240. Fluorescence measurements taken at 16 or 24 hours were corrected for optical density.
		+
		+
		+	{\| border="1" cellpadding="2"
		+	!width="30"\|Sample
		+	!width="30"\|Fluorescence
		+	!width="300"\|Optical Density
		+	!width="30"\|Fluor/OD
		+	\|-
		+	\| LB only\|\| 2\|\| 0.043\|\| 46.5
		+	\|-
		+	\|}

	16 hr.		16 hr.

@@ Line 1: / Line 1: @@
-'''Cell-to-cell signaling by XOR gate constructs'''
+'''Cell-to-cell signaling by XOR gate constructs''' - The ability of two different XOR gate constructs to send a signal via the Lux system was measured by coplating bacterial XOR clones with bacteria containing a Lux receiver construct.
 Positive control using a Lux sender and a Lux receiver (K09100).  Green fluorescence indicates LuxI expression in the Lux sender and receipt of the intercellular signal by the receiver.
 [[Image:9100_Sender.JPG|389px]]
 XOR gate construct K091173 coplated with the K09100 Lux receiver.  Green fluorescence indicates that at least one of the two hybrid promoters in the XOR construct is driving expression of the LuxI gene.
 [[Image:9100_K82.JPG|389px]]
 XOR gate construct K091182 coplated with the K09100 Lux receiver.  Green fluorescence indicates that at least one of the two hybrid promoters in the XOR construct is driving expression of the LuxI gene.
 [[Image:9100_K73.JPG|389px]]
 '''Improved function by a modified Lac promoter'''