Extending theophylline application - Revision history

Shdoherty at 18:28, 3 July 2014

2014-07-03T18:28:35Z

Shdoherty at 18:00, 3 July 2014

2014-07-03T18:00:12Z

Jolim: Added a link to Jon Lim's protocol for finding the optimal caffeine concentration on plates

2014-06-19T19:41:02Z

Added a link to Jon Lim's protocol for finding the optimal caffeine concentration on plates

Tetaye at 18:43, 27 May 2014

2014-05-27T18:43:02Z

Tetaye at 18:42, 27 May 2014

2014-05-27T18:42:17Z

Tetaye at 15:38, 27 May 2014

2014-05-27T15:38:16Z

Duatchley at 13:05, 21 May 2014

2014-05-21T13:05:04Z

Duatchley at 13:04, 21 May 2014

2014-05-21T13:04:32Z

Macampbell: Blanked the page

2014-05-21T12:44:41Z

Blanked the page

Macampbell at 12:43, 21 May 2014

2014-05-21T12:43:11Z

← Older revision		Revision as of 18:28, 3 July 2014
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	[[File:Finalgraph.docx]] (Shannon Doherty)		[[File:Finalgraph.docx]] (Shannon Doherty)
		+
		+	updated graph here [[File:Final.docx]]
		+

	[[Optimal caffeine concentration on plates]] (Jon Lim)		[[Optimal caffeine concentration on plates]] (Jon Lim)

@@ Line 11: / Line 11: @@
 '''Broth experiment:'''
 If one clone really is the best, then test it in broth to make the experiment/results more versatile. If it is the best, it should dominate the broth very quickly.
 [[Optimal caffeine concentration on plates]] (Jon Lim)

@@ Line 11: / Line 11: @@
 '''Broth experiment:'''
 If one clone really is the best, then test it in broth to make the experiment/results more versatile. If it is the best, it should dominate the broth very quickly.
 '''Questions:'''

@@ Line 34: / Line 34: @@
   The superstar combination #14
 tests in each campus
 st test tube- broth, tet, chlor and amp
 nd test tube- broth, tet, chlor, amp and
 	-Broth, tet
 	-Growth with theophylline, tet resistance
@@ Line 42: / Line 44: @@
   Question is growth
 Two test-tube with all 24 clones
 st tube- tet and amp
 nd tube tet, chlor and amp

@@ Line 25: / Line 25: @@
-.	Determine growth in the Ecoli strain
 -Wild type in broth at 37 degrees Celsius
 -Grow them 12-14 hours
-. The superstar combination #14
 tests in each campus
 st test tube- broth, tet, chlor and amp
@@ Line 35: / Line 39: @@
 	-Growth with theophylline, tet resistance
 	-no growth, demetylase problem
-. Question is growth
 Two test-tube with all 24 clones
 st tube- tet and amp
 nd tube tet, chlor and amp
-. 6 tubes of all combinations
 chlor not run on the last chaperone group
-. Theophylline
+Theophylline control
 Liter LB+4mM

← Older revision		Revision as of 15:38, 27 May 2014
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	Is there a way to figure out which will be more dominant even when it isn’t dominant yet? Is there some sort of trend or flag that we can identify that would indicate a population that will eventually be dominant?		Is there a way to figure out which will be more dominant even when it isn’t dominant yet? Is there some sort of trend or flag that we can identify that would indicate a population that will eventually be dominant?
		+
		+
		+
		+
		+	1. Determine growth in the Ecoli strain
		+	-Wild type in broth at 37 degrees Celsius
		+	-Grow them 12-14 hours
		+	2. The superstar combination #14
		+	2 tests in each campus
		+	1st test tube- broth, tet, chlor and amp
		+	2nd test tube- broth, tet, chlor, amp and
		+	-Broth, tet
		+	-Growth with theophylline, tet resistance
		+	-no growth, demetylase problem
		+	3. Question is growth
		+	Two test-tube with all 24 clones
		+	1st tube- tet and amp
		+	2nd tube tet, chlor and amp
		+	4. 6 tubes of all combinations
		+	chlor not run on the last chaperone group
		+
		+	5. Theophylline
		+
		+	1Liter LB+4mM
		+	1mL(50ug/mL Amp)
		+	4mL(5mg/mL Tet)
		+	1mL(35ug/mL Chloro)
		+
		+	Cells- 120 mL cells each time

@@ Line 1: / Line 1: @@
-How can the caffeine disk results be extended? What experiments can be done?
+'''How can the caffeine disk results be extended? What experiments can be done?'''
 Redo original experiment to verify results
-Natural selection experiment:
+'''Natural selection experiment:'''
 Plate all clones on one plate and then plate clones in groups of 5 on 4 plates to see if there are other natural factors affecting growth that we haven’t considered.
-Finding the best clone:
+'''Finding the best clone:'''
 Plate clones in groups of 5 on 4 plates in the presence of caffeine (varying levels for varying experiments). Take the best clone from each plate and plate them on one plate to see the best (all taking into account any additional natural variables that need to be monitored or accounted for).
-Broth experiment:
+'''Broth experiment:'''
 If one clone really is the best, then test it in broth to make the experiment/results more versatile. If it is the best, it should dominate the broth very quickly.
-Questions:
+'''Questions:'''
 What if the best fitness module is in a naturally failing e coli (due to factors we have not considered or measured)?

← Older revision	Revision as of 13:04, 21 May 2014
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	+	How can the caffeine disk results be extended? What experiments can be done?

	+	Redo original experiment to verify results
	+
	+	Natural selection experiment:
	+	Plate all clones on one plate and then plate clones in groups of 5 on 4 plates to see if there are other natural factors affecting growth that we haven’t considered.
	+
	+	Finding the best clone:
	+	Plate clones in groups of 5 on 4 plates in the presence of caffeine (varying levels for varying experiments). Take the best clone from each plate and plate them on one plate to see the best (all taking into account any additional natural variables that need to be monitored or accounted for).
	+
	+	Broth experiment:
	+	If one clone really is the best, then test it in broth to make the experiment/results more versatile. If it is the best, it should dominate the broth very quickly.
	+
	+	Questions:
	+
	+	What if the best fitness module is in a naturally failing e coli (due to factors we have not considered or measured)?
	+
	+	Should our future experiments focus on optimization (if we already have a good system) or on diversification (broth, other common lab situations that would be easier for someone to use than plates, etc). Do we care which is best or do we want an easy process that is sufficiently good?
	+
	+	Is more prep time worth a better result (financially)?
	+
	+	Is there a way to figure out which will be more dominant even when it isn’t dominant yet? Is there some sort of trend or flag that we can identify that would indicate a population that will eventually be dominant?

← Older revision		Revision as of 12:44, 21 May 2014
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← Older revision		Revision as of 12:43, 21 May 2014
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−	~~[http://www.bio.davidson.edu/Campbell My home page]~~