Figure 6. Modularity of the AND gate. - Revision history

Emgarren at 05:35, 20 November 2007

2007-11-20T05:35:06Z

2007-11-20T05:34:44Z

2007-11-20T05:30:53Z

2007-11-20T05:29:54Z

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<b>Figure 6. Modularity of the AND gate.</b>

(Image from Figure 6 of Anderson et al., 2007. Permission pending.)

[[Image:AND_gate_fig_6.jpg]]

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	B. Exchanging the output for the ''inv'' gene, coding for invasin, a protein that allows bacteria to invade mammalian cells. When both input promoters are on (in the presence of Sal and magnesium limitation), the cells exhibit the same level of invasiveness as if the cells were programmed with the ''inv'' gene placed under a constitutive promoter.		B. Exchanging the output for the ''inv'' gene, coding for invasin, a protein that allows bacteria to invade mammalian cells. When both input promoters are on (in the presence of Sal and magnesium limitation), the cells exhibit the same level of invasiveness as if the cells were programmed with the ''inv'' gene placed under a constitutive promoter.
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		+	[[Image:AND_gate_fig_6.jpg]]

	(Image from Figure 6 of Anderson et al., 2007. Permission pending.)		(Image from Figure 6 of Anderson et al., 2007. Permission pending.)
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 The modularity of the AND gate is demonstrated by reconnecting the gate to new inputs (natural promoters), and a new output (desired phenotype).
-A.  Exchanging the inputs:
+A.  Exchanging the inputs:  Input 1: The ''lux'' promoter, which responds to the quorum signal AI-1.  Input 2: The ''mgrB'' promoter, which responds to the absence of exogenous magnesium via the PhoPQ two-component system.  Only when both promoters are on (in the presence of AI-1 and magnesium limitation) is the output on.
-B. Exchanging the output:
+B. Exchanging the output for the ''inv'' gene, coding for invasin, a protein that allows bacteria to invade mammalian cells.  When both input promoters are on (in the presence of Sal and magnesium limitation), the cells exhibit the same level of invasiveness as if the cells were programmed with the ''inv'' gene placed under a constitutive promoter.
 (Image from Figure 6 of Anderson et al., 2007.  Permission pending.)
 [[Image:AND_gate_fig_6.jpg]]