Future Design/ 8+ HPP - Revision history

Cadoyle at 19:38, 20 June 2011

2011-06-20T19:38:20Z

Cadoyle at 13:58, 10 June 2011

2011-06-10T13:58:31Z

Cadoyle at 12:55, 10 June 2011

2011-06-10T12:55:44Z

Cadoyle at 18:10, 9 June 2011

2011-06-09T18:10:17Z

Cadoyle at 17:47, 9 June 2011

2011-06-09T17:47:52Z

Cadoyle: Created page with 'Idea: We were thinking that if we could get one strain of E.coli that is auxotrophic to all 20 amino acids we could split the amino acids and then follow our designed protocol a…'

2011-06-09T17:47:14Z

Created page with 'Idea: We were thinking that if we could get one strain of E.coli that is auxotrophic to all 20 amino acids we could split the amino acids and then follow our designed protocol a…'

New page

Idea:

We were thinking that if we could get one strain of E.coli that is auxotrophic to all 20 amino acids we could split the amino acids and then follow our designed protocol and solve a 20 HPP at one time.

However, we could not find any strains of E. coli that are auxotrophic for all 20 amino acids. We were able to find 3 strains auxotrophic for at least two amino acids. So, we thought that we could mate then E. coli cells and get a strain of E. coli auxotrophic for 8 amino acids.

As a result, we need to find and obtain:

{|border="1" cellpadding="5" cellspacing="0"
|-
! scope="col" style="background:#efefef;" | What We Need
! scope="col" style="background:#efefef;" | Status
|-
| Y10 E. coli Strain || Found at Yale University, but have not requested a copy Picture of Yale University E.coli database results: [[Media:Yale ]]<br>||
|-
|58-161 E.coli Strain || Found at Yale University, but have not requested a copy||
|-
| WWU E. coli Strain || Found the person (R. C. Bockrath) who designed it, but he deceased, so am sending an email to the chair of the depart he worked for at Indiana University to see if there are copies available ||
|-
|Protocol for mating E. coli || [[Protocol Options ]]<br>||
|-
| Protocol for changing a haploid to a diploid || Still searching for one ||
|}

@@ Line 22: / Line 22: @@
 | Protocol for changing a haploid to a diploid || HrF cross will work for mating protocol to get haploid ||
 |}
 Mutated Enzymes Causing Auxotroph's for Amino Acids: [[Media:AminoAcidAuxotrophs.pptx]] <br>

← Older revision		Revision as of 12:55, 10 June 2011
Line 22:		Line 22:
	\| Protocol for changing a haploid to a diploid \|\| Still searching for one \|\|		\| Protocol for changing a haploid to a diploid \|\| Still searching for one \|\|
	\|}		\|}
		+
		+
		+	Mutated Enzymes Causing Auxotroph's for Amino Acids: [[Media:AminoAcidAuxotrophs.pptx]] <br>

@@ Line 16: / Line 16: @@
 |58-161 E.coli Strain || Found at Yale University, but have not requested a copy||
 |-
-| WWU E. coli Strain || Found the person (R. C. Bockrath) who designed it, but he deceased, so am sending an email to the chair of the depart he worked for at Indiana University to see if there are copies available ||
+| WWU E. coli Strain || Found at Yale University is call E. coli strain 15 T-, but have not requested a copy ||
 |-
 |Protocol for mating E. coli  || [[Protocol Options ]]<br>||
 |-
-| Protocol for changing a haploid to a diploid || Still searching for one ||
+| Protocol for changing a haploid to a diploid || HrF cross will work for mating protocol to get haploid ||
 |}
 Mutated Enzymes Causing Auxotroph's for Amino Acids: [[Media:AminoAcidAuxotrophs.pptx]] <br>

@@ Line 3: / Line 3: @@
 We were thinking that if we could get one strain of E.coli that is auxotrophic to all 20 amino acids we could split the amino acids and then follow our designed protocol and solve a 20 HPP at one time.
-However, we could not find any strains of E. coli that are auxotrophic for all 20 amino acids. We were able to find 3 strains auxotrophic for at least two amino acids. So, we thought that we could mate then E. coli cells and get a strain of E. coli auxotrophic for 8 amino acids.
+However, we could not find any strains of E. coli that are auxotrophic for all 20 amino acids. We were able to find 3 strains auxotrophic for at least two amino acids. So, we thought that we could mate the E. coli cells and get a strain of E. coli auxotrophic for 8 amino acids.
 As a result, we need to find and obtain:

@@ Line 12: / Line 12: @@
 ! scope="col" style="background:#efefef;" | Status
 |-
-| Y10 E. coli Strain || Found at Yale University, but have not requested a copy        Picture of Yale University E.coli database results: [[Media:Yale ]]<br>||
+| Y10 E. coli Strain || Found at Yale University, but have not requested a copy        Picture of Yale University E.coli database results: [[Media:Yale.png ]]<br>||
 |-
 |58-161 E.coli Strain || Found at Yale University, but have not requested a copy||