https://gcat.davidson.edu/GcatWiki/index.php?action=history&feed=atom&title=J-GGA_Promoter_Sequence 2026-04-16T22:29:12Z Revision history for this page on the wiki MediaWiki 1.28.2 https://gcat.davidson.edu/GcatWiki/index.php?title=J-GGA_Promoter_Sequence&diff=16503&oldid=prev 2013-06-11T15:44:45Z

<p>Created page with &quot;Design of promoters for use in J-GGA Scaffold Promoters Used P5 highest J119031 BBa_J119031 Part-only sequence (36 bp) ttgacaattaatcatccggctcgtaatttatgtgga P14 medium J11...&quot;</p> <p><b>New page</b></p><div>Design of promoters for use in J-GGA Scaffold<br /> <br /> Promoters Used<br /> <br /> P5 highest J119031<br /> <br /> BBa_J119031 Part-only sequence (36 bp)<br /> <br /> ttgacaattaatcatccggctcgtaatttatgtgga<br /> <br /> P14 medium J119033<br /> <br /> BBa_J119033 Part-only sequence (36 bp)<br /> <br /> ttgacaattaatcatccggctcgtataatgtgtgga<br /> <br /> P9 lower J119032<br /> <br /> BBa_J119032 Part-only sequence (35 bp)<br /> <br /> ttgcctcttaatcatcggctcgtataatgtgtgga<br /> <br /> Aligned (J31,32, and 33)<br /> <br /> BBa_J119031 ttgacaattaatcatccggctcgtaatttatgtgga<br /> <br /> BBa_J119033 ttgacaattaatcatccggctcgtataatgtgtgga<br /> <br /> BBa_J119032 ttgcctcttaatcatcggctcgtatwaatgtgtgga<br /> <br /> Consensus TTGMCWMTTAATCATCSGSYYSKWWWWWTRTGTGGA<br /> <br /> <br /> Number of Sequences = 2 power 15 = 32,768<br /> <br /> Aligned (J31 and 32 only)<br /> <br /> BBa_J119031 ttgacaattaatcatccggctcgtaatttatgtgga<br /> <br /> BBa_J119033 ttgacaattaatcatccggctcgtataatgtgtgga<br /> <br /> Consensus TTGACAATTAATCATCCGGCTCGTAWWWTRTGTGGA<br /> <br /> Number of Sequences = 2 power 4 = 16<br /> <br /> New J-GGA Scaffold (after PCR mutagenesis with above primers)<br /> <br /> Prefix: Gaattcgcggccgcttctagag<br /> <br /> Junction A: Atagtctgttcatggtgc<br /> <br /> Spacer: acgt<br /> <br /> Junction B: ggctgatgtagttaaggc<br /> <br /> Spacer: cgga<br /> <br /> Junction C: gttctaatggggaccatc<br /> <br /> Spacer: ttag<br /> <br /> Junction D: cggagctatgtattcctg<br /> <br /> Suffix: tactagtagcggccgctgcag<br /> <br /> Sticky ends needed:<br /> <br /> Junction A: GTGC<br /> <br /> Junction B: GGCT<br /> <br /> BBA_J31 GTGCTTGACAATTAATCATCCGGCTCGTAATTTATGTGGA<br /> <br /> AACTGTTAATTAGTAGGCCGAGCATTAAATACACCTTCGG<br /> <br /> BBA_J33 GTGCTTGACAATTAATCATCCGGCTCGTATAATGTGTGGA<br /> <br /> AACTGTTAATTAGTAGGCCGAGCATATTACACACCTTCGG<br /> <br /> CONS GTGCTTGACAATTAATCATCCGGCTCGTAWWWTRTGTGGA<br /> <br /> AACTGTTAATTAGTAGGCCGAGCATWWWAYACACCTTCGG<br /> <br /> <br /> Oligos to be Ordered<br /> <br /> J31top;GTGCTTGACAATTAATCATCCGGCTCGTAATTTATGTGGA<br /> <br /> J31bot; GGCTTCCACATAAATTACGAGCCGGATGATTAATTGTCAA<br /> <br /> J33top; GTGCTTGACAATTAATCATCCGGCTCGTATAATGTGTGGA<br /> <br /> <br /> J33bot; GGCTTCCACACATTATACGAGCCGGATGATTAATTGTCAA<br /> <br /> J31+32top; GTGCTTGACAATTAATCATCCGGCTCGTAWWWTRTGTGGA<br /> <br /> J31+32bot; GGCTTCCACAYAWWWTACGAGCCGGATGATTAATTGTCAA</div>

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