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J-GGA Scaffold Sequence - Revision history
2026-04-16T22:28:54Z
Revision history for this page on the wiki
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https://gcat.davidson.edu/GcatWiki/index.php?title=J-GGA_Scaffold_Sequence&diff=16501&oldid=prev
Spolpitiyaarachchige: Created page with "J-GGA Scaffold Oligos Prefix: Gaattcgcggccgcttctagag Junction A: Atagtctgttcatggtgc Spacer: acgt Junction B: Tattgatgtagttaaggc Spacer: cgga Junction C: Tttctaatggggacca..."
2013-06-11T15:42:59Z
<p>Created page with "J-GGA Scaffold Oligos Prefix: Gaattcgcggccgcttctagag Junction A: Atagtctgttcatggtgc Spacer: acgt Junction B: Tattgatgtagttaaggc Spacer: cgga Junction C: Tttctaatggggacca..."</p>
<p><b>New page</b></p><div>J-GGA Scaffold Oligos<br />
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Prefix: Gaattcgcggccgcttctagag<br />
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Junction A: Atagtctgttcatggtgc<br />
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Spacer: acgt<br />
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Junction B: Tattgatgtagttaaggc<br />
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Spacer: cgga<br />
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Junction C: Tttctaatggggaccatc<br />
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Spacer: ttag<br />
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Junction D: Ttaagctatgtattcctg<br />
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Suffix: tactagtagcggccgctgcag<br />
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Top Strand<br />
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SCAFF_TOP1; AATTCGCGGCCGCTTCTAGAGATAGTCTGTTCATGGTGCACGTTATTGATGTAGTTAAGGCCGGATTTCTAATGG<br />
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SCAFF_TOP2; GGACCATCTTAGTTAAGCTATGTATTCCTGTACTAGTAGCGGCCGCTGCA<br />
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BOTTOM STRAND (5’ TO 3’)<br />
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SCAFF_BOT1; GCGGCCGCTACTAGTACAGGAATACATAGCTTAACTAAGATGGTCCCCATTAGAAATCCGGCCTTAACTAC<br />
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SCAFF_BOT2; ATCAATAACGTGCACCATGAACAGACTATCTCTAGAAGCGGCCGCG<br />
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Annealed AATTCGCGGCCGCTTCTAGAGATAGTCTGTTCATGGTGCACGTTATTGATGTAGTTAAGGCCGGATTTCTAATGGGGACCATCTTAGTTAAGCTATGTATTCCTGTACTAGTAGCGGCCGCTGCA<br />
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GCGCCGGCGAAGATCTCTATCAGACAAGTACCACGTGCAATAACTACATCAATTCCGGCCTAAAGATTACCCCTGGTAGAATCAATTCGATACATAAGGACATGATCATCGCCGGCG</div>
Spolpitiyaarachchige