https://gcat.davidson.edu/GcatWiki/index.php?action=history&feed=atom&title=Las
Las - Revision history
2026-04-23T14:33:57Z
Revision history for this page on the wiki
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https://gcat.davidson.edu/GcatWiki/index.php?title=Las&diff=6986&oldid=prev
BoCool at 15:13, 28 October 2008
2008-10-28T15:13:21Z
<p></p>
<p><b>New page</b></p><div>== Las/Rhl cell signaling system ==<br />
'''Responsible''': Robert Cool, Alicia Allen, and Erin Feeney<br />
<br />
'''Las System'''<br />
<br />
'''Signal Molecule''': An AHL called PAI-1 (N-3-oxododecanoyl-l-hsl)(3-oxo-C12-hsl)<br />
<br />
'''Bacterial species''': Pseudomonas aeruginosa gram(-) possibly E.coli (see article 3)<br />
<br />
'''Receiver protein''': LasR<br />
<br />
'''Effect of binding''': TXN activation of virulence genes, lasA, lasB, apr, toxR<br />
<br />
'''Synthase''': LasI enzyme<br />
<br />
'''Target Genes''': lasI, lasA, lasB, apr, toxR<br />
<br />
'''Regulation''': unknown<br />
<br />
<br />
'''References'''<br />
<br />
"Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes" <br />
<br />
JP Pearson, EC Pesci and BH Iglewski <br />
[http://jb.asm.org/cgi/reprint/179/18/5756?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&titleabstract=las&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT]<br />
<br />
<br />
"Regulation of Pseudomonas Quinolone Signal Synthesis in Pseudomonas aeruginosa"<br />
<br />
Dana S. Wade, M. Worth Calfee, Edson R. Rocha, Elizabeth A. Ling, Elana Engstrom, James P. Coleman, and Everett C. Pesci<br />
[http://jb.asm.org/cgi/content/full/187/13/4372?view=long&pmid=15968046 2]<br />
<br />
<br />
Posttranscriptional Control of Quorum-Sensing-Dependent Virulence Genes by DksA in Pseudomonas aeruginosa<br />
<br />
Florence Jude,Thilo Köhler,Pavel Branny,Karl Perron,Matthias P. Mayer,Rachel Comte, and Christian van Delden<br />
[http://jb.asm.org/cgi/content/full/185/12/3558?view=long&pmid=12775693 3]<br />
<br />
Pending: [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1447470]<br />
<br />
'''Rhl System''' <br />
<br />
<br><br />
'''Signal Molecule''': An AHL called PAI-2, Plasminogen activator inhibitor-2, N-butanoyl-homoserine lactone (C4HSL) <br />
<br />
'''Bacterial species''': Pseudomonas aeruginosa, gram(-)<br />
<br />
'''Receiver Protein''': Rhl R <br />
<br />
'''Effect of Binding''': activation of Rhamnosyl Transferase, then making RL (rhamnolipid) <br />
<br />
'''Synthase''': RhlA and RhlB <br />
<br />
'''Target Genes''': pqsABCDE and phnAB<br />
<br />
'''Regulation''': unknown<br />
<br />
'''References'''<br />
<br />
[http://jb.asm.org/cgi/reprint/189/13/4827 background information on Las and Rhl]<br />
<br />
[[Image:Las_rhl.gif]]<br />
<br />
'''Parts Needed''':<br />
<br />
LasR + pro/term<br />
<br />
RhlR + pro/term<br />
<br />
RhlI + pro/term<br />
<br />
pqsABCDE + pro/term<br />
<br />
pqsR<br />
<br />
pqsH + pro/term<br />
<br />
phnAB<br />
<br />
LasI<br />
<br />
RBS</div>
BoCool