LasR/cI - Revision history

BoCool at 18:24, 22 July 2008

2008-07-22T18:24:12Z

BoCool at 18:21, 22 July 2008

2008-07-22T18:21:57Z

BoCool at 18:18, 22 July 2008

2008-07-22T18:18:18Z

BoCool at 18:17, 22 July 2008

2008-07-22T18:17:41Z

XiZhu at 15:58, 17 July 2008

2008-07-17T15:58:25Z

XiZhu at 18:30, 15 July 2008

2008-07-15T18:30:21Z

AlAllen at 15:57, 10 July 2008

2008-07-10T15:57:40Z

XiZhu at 11:48, 10 July 2008

2008-07-10T11:48:04Z

XiZhu at 20:58, 1 July 2008

2008-07-01T20:58:46Z

New page

pBAD+RBS+lasR+TT+cI+TT+pLasR/cI+RBS+LuxI+RBS+Mnt+TT

I13453+B0034+C0079+B0015+C0051+B0034+C0061+B0034+C0072+B0015

@@ Line 29: / Line 29: @@
 | 1|| K091141||RBS+Mnt+TT  ||R/X;S/P  ||R/X ||468+2079 ||C0261||RBS+luxI  ||R/X;S/P  ||R/X  ||661 ||K091154 ||1137
 |-
-| 2||K091143||plasR/cI ||R/X;S/P ||S/P ||164+2079 ||K091154||RBS+luxI+RBS+Mnt+TT ||R/X; S/P  ||X/P  ||1137 ||TBD ||
+| 2||K091143||plasR/cI ||R/X;S/P ||S/P ||164+2079 ||K091154||RBS+luxI+RBS+Mnt+TT ||R/X; S/P  ||X/P  ||1137 ||K091155 ||1309
 |-
 |}

@@ Line 27: / Line 27: @@
 |'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''
 |-
-| 1|| K091141||RBS+Mnt+TT  ||R/X;S/P  ||R/X ||468+2079 ||C0261||RBS+luxI  ||R/X;S/P  ||R/X  ||661 ||TBD ||
+| 1|| K091141||RBS+Mnt+TT  ||R/X;S/P  ||R/X ||468+2079 ||C0261||RBS+luxI  ||R/X;S/P  ||R/X  ||661 ||K091154 ||1137
 |-
-| 2||K091143||plasR/cI ||R/X;S/P ||S/P ||164+2079 ||TBD||RBS+luxI+RBS+Mnt+TT ||R/X; S/P  ||X/P  || ||TBD ||
+| 2||K091143||plasR/cI ||R/X;S/P ||S/P ||164+2079 ||K091154||RBS+luxI+RBS+Mnt+TT ||R/X; S/P  ||X/P  ||1137 ||TBD ||
 |-
 |}

@@ Line 7: / Line 7: @@
 '''pLasR/cI+RBS+LuxI+RBS+Mnt+TT'''
-'''K091141'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''
+'''K091143'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''
 '''Ligation Plan'''
@@ Line 29: / Line 29: @@
 | 1|| K091141||RBS+Mnt+TT  ||R/X;S/P  ||R/X ||468+2079 ||C0261||RBS+luxI  ||R/X;S/P  ||R/X  ||661 ||TBD ||
 |-
-| 2||K09114||plasR/cI ||R/X;S/P ||S/P ||164+2079 ||TBD||RBS+luxI+RBS+Mnt+TT ||R/X; S/P  ||X/P  || ||TBD ||
+| 2||K091143||plasR/cI ||R/X;S/P ||S/P ||164+2079 ||TBD||RBS+luxI+RBS+Mnt+TT ||R/X; S/P  ||X/P  || ||TBD ||
 |-
 |}
@@ Line 46: / Line 46: @@
 |[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07  ||Plt.1-23J ||pSB1A2 ||
 |-
-|[http://partsregistry.org/Part:BBa_K091141 K091141] ||plasR/cI ||MW ||N/A ||pSB1A2 ||
+|[http://partsregistry.org/Part:BBa_K091143 K091143] ||plasR/cI ||MW ||N/A ||pSB1A2 ||
 |-
 |[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07  ||Plt.1-24A ||pSB1A2 ||
 |-
 |}

@@ Line 7: / Line 7: @@
 '''pLasR/cI+RBS+LuxI+RBS+Mnt+TT'''
-'''K09110?'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''
+'''K091141'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''
 '''Ligation Plan'''
@@ Line 27: / Line 27: @@
 |'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''
 |-
-| 1|| K091143 ||plasR/cI ||R/X; S/P||S/P || 164+2079||C0261||RBS+luxI  ||R/X;S/P  ||X/P  ||661 ||TBD ||
+| 1|| K091141||RBS+Mnt+TT  ||R/X;S/P  ||R/X ||468+2079 ||C0261||RBS+luxI  ||R/X;S/P  ||R/X  ||661 ||TBD ||
 |-
-| 2|| B0015 ||TT ||R/X; S/P||R/X || 129+2079||I13025||RBS+Mnt  ||R/X;S/P  ||R/S  ||331 ||TBD ||
+| 2||K09114||plasR/cI ||R/X;S/P ||S/P ||164+2079 ||TBD||RBS+luxI+RBS+Mnt+TT ||R/X; S/P  ||X/P  || ||TBD ||
 |-
 |}
@@ Line 48: / Line 46: @@
 |[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07  ||Plt.1-23J ||pSB1A2 ||
 |-
-|[http://partsregistry.org/Part:BBa_K09110? K09110?] ||plasR/cI ||MW ||N/A ||pSB1A2 ||
+|[http://partsregistry.org/Part:BBa_K091141 K091141] ||plasR/cI ||MW ||N/A ||pSB1A2 ||
 |-
 |[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07  ||Plt.1-24A ||pSB1A2 ||
 |-
 |}

@@ Line 31: / Line 31: @@
 | 2|| B0015 ||TT ||R/X; S/P||R/X || 129+2079||I13025||RBS+Mnt  ||R/X;S/P  ||R/S  ||331 ||TBD ||
 |-
-| 3||TBD||RBS+Mnt+TT ||R/X;S/P ||R/X || ||TBD||plasR/cI+RBS+luxI ||R/X; S/P  ||R/S  || ||TBD ||
+| 3||K091141||RBS+Mnt+TT ||R/X;S/P ||R/X ||468+2079 ||TBD||plasR/cI+RBS+luxI ||R/X; S/P  ||R/S  || ||TBD ||
 |-
 |}

← Older revision		Revision as of 15:57, 10 July 2008
Line 1:		Line 1:
−	~~pBAD+RBS+lasR+RBS+cI+TT+pLasR/cI+RBS+LuxI+RBS+Mnt+TT~~	+	'''Goal:'''

−	I13453+B0034+C0079+~~B0034~~+~~C0051~~+B0034+C0061+B0034+C0072+B0015	+	'''pBAD+RBS+lasR+TT'''(James DC)
		+
		+	'''S0388?'''(I13453+B0034+C0079+B0015)
		+
		+	'''pLasR/cI+RBS+LuxI+RBS+Mnt+TT'''
		+
		+	'''K09110?'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''
		+
		+	'''Ligation Plan'''
		+	{\| border="1" cellpadding="2"
		+	!width="20"\|
		+	!width="30"\|Vector
		+	!width="110"\|
		+	!width="30"\|
		+	!width="30"\|
		+	!width="30"\|
		+	!width="30"\|Insert
		+	!width="110"\|
		+	!width="30"\|
		+	!width="30"\|
		+	!width="30"\|
		+	!width="30"\|Product
		+	!width="30"\|
		+	\|-
		+	\|'''Step'''\|\| '''BBa_''' \|\| '''Description''' \|\|''' Pre-/suffix''' \|\|''' Enzymes '''\|\|'''Size '''\|\|'''BBa_'''\|\|'''Description'''\|\|'''Pre-/suffix'''\|\|'''Enzymes'''\|\|'''Size'''\|\|'''BBa_'''\|\|'''Size'''
		+	\|-
		+	\| 1\|\| K09110? \|\|plasR/cI \|\|R/X; S/P\|\|S/P \|\| 215+2079\|\|C0261\|\|RBS+luxI \|\|R/X;S/P \|\|X/P \|\|661 \|\|TBD \|\|
		+	\|-
		+	\| 2\|\| B0015 \|\|TT \|\|R/X; S/P\|\|R/X \|\| 129+2079\|\|I13025\|\|RBS+Mnt \|\|R/X;S/P \|\|R/S \|\|331 \|\|TBD \|\|
		+	\|-
		+	\| 3\|\|TBD\|\|RBS+Mnt+TT \|\|R/X;S/P \|\|R/X \|\| \|\|TBD\|\|plasR/cI+RBS+luxI \|\|R/X; S/P \|\|R/S \|\| \|\|TBD \|\|
		+	\|-
		+	\|}
		+
		+	'''Parts'''
		+	{\| border="1" cellpadding="2"
		+	!width="40"\|BBa_
		+	!width="110"\|Description
		+	!width="60"\|Source
		+	!width="100"\|location('07)
		+	!width="35"\|Vector
		+	!width="40"\|Responsible
		+	\|-
		+	\|[http://partsregistry.org/Part:BBa_B0015 B0015]\|\|double terminator \|\|registry 07 \|\|Plt.1-1I;Plt.3-3O \|\|pSB1AK3 \|\|
		+	\|-
		+	\|[http://partsregistry.org/Part:BBa_I13025 I13025] \|\|RBS+Mnt \|\|registry 07 \|\|Plt.1-23J \|\|pSB1A2 \|\|
		+	\|-
		+	\|[http://partsregistry.org/Part:BBa_K09110? K09110?] \|\|plasR/cI \|\|MW \|\|N/A \|\|pSB1A2 \|\|
		+	\|-
		+	\|[http://partsregistry.org/Part:BBa_C0261 C0261] \|\|RBS+luxI \|\|registry 07 \|\|Plt.1-24A \|\|pSB1A2 \|\|
		+	\|-
		+	\|}

← Older revision		Revision as of 11:48, 10 July 2008
Line 1:		Line 1:
−	pBAD+RBS+lasR+TT+cI+TT+pLasR/cI+RBS+LuxI+RBS+Mnt+TT	+	pBAD+RBS+lasR+RBS+cI+TT+pLasR/cI+RBS+LuxI+RBS+Mnt+TT

−	I13453+B0034+C0079+~~B0015~~+C0051+B0034+C0061+B0034+C0072+B0015	+	I13453+B0034+C0079+B0034+C0051+B0034+C0061+B0034+C0072+B0015