Laura Voss - Synthetic Biology Seminar - Revision history

Lavoss: /* Class Wiki Project */

2007-11-20T13:29:17Z

‎Class Wiki Project

Lavoss at 13:26, 20 November 2007

2007-11-20T13:26:14Z

Lavoss: /* Links for "Simulation of GFP Expression" */

2007-10-22T06:33:56Z

‎Links for "Simulation of GFP Expression"

Lavoss: /* Links for "Simulation of GFP Expression" */

2007-10-20T22:36:34Z

‎Links for "Simulation of GFP Expression"

Lavoss: /* Links for "Simulation of GFP Expression" */

2007-10-20T22:35:01Z

‎Links for "Simulation of GFP Expression"

Lavoss: /* Links for "Simulation of GFP Expression" */

2007-10-20T22:11:48Z

‎Links for "Simulation of GFP Expression"

Lavoss: /* Paper Discussion 10/23 */

2007-10-20T21:45:58Z

‎Paper Discussion 10/23

Lavoss: /* Paper Discussion 10/23 */

2007-10-20T21:18:54Z

‎Paper Discussion 10/23

Lavoss: /* Paper Discussion 10/23 */

2007-10-18T05:39:32Z

‎Paper Discussion 10/23

Lavoss: /* Laura Voss - Synthetic Biology Seminar */

2007-10-18T05:37:59Z

‎Laura Voss - Synthetic Biology Seminar

← Older revision		Revision as of 13:29, 20 November 2007
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	*[http://en.wikipedia.org/wiki/Lineweaver-Burk_plot Lineweaver-Burk Plots] are a way of graphically representing Michaelis-Menten kinetic equations. Prior to the introduction of computer software that could easily calculate enzyme parameters, Lineweaver-Burk plots were frequently used to calculate such values as the maximum rate of reaction and the Michaelis constant. However, as the plots are not as accurate as computer calculations, they are now primarily used to represent kinetic data rather than calculate it.		*[http://en.wikipedia.org/wiki/Lineweaver-Burk_plot Lineweaver-Burk Plots] are a way of graphically representing Michaelis-Menten kinetic equations. Prior to the introduction of computer software that could easily calculate enzyme parameters, Lineweaver-Burk plots were frequently used to calculate such values as the maximum rate of reaction and the Michaelis constant. However, as the plots are not as accurate as computer calculations, they are now primarily used to represent kinetic data rather than calculate it.

−	== ~~Class Wiki Project~~ ==	+	== [[Promoters and Reporters in Synthetic Biology]] ==

← Older revision		Revision as of 13:26, 20 November 2007
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	== Class Wiki Project ==		== Class Wiki Project ==
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−	~~My class project will be on the design of temperature-sensing cellular mechanisms, such as cellular thermometers.~~

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 Prior to this paper's publication, the degradation of GFP had been calculated using first-order kinetics. In that case, the degradation of GFP (whether it was fluorescing or not) would have been degraded at a constant rate, and would degrade faster the more protein was present. In theory, the degradation rate could increase indefinitely, so long as the amount of protein continued to increase. In a Michaelis-Menten equation, there is a maximum rate of degradation that an enzyme approaches as the concentration of substrate increases; however, it never ''reaches'' this maximum rate. So, after a certain concentration of GFP is reached, the degradation rate remains almost constant no matter how great the concentration of GFP. This can affect how much fluorescence is perceived in a cell and whether it corresponds to promoter activity.
 == Class Wiki Project ==
 My class project will be on the design of temperature-sensing cellular mechanisms, such as cellular thermometers.

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 *[http://en.wikipedia.org/wiki/Michaelis-Menten_kinetics Michaelis-Menten Kinetics] is a system of equations that describes the kinetics of enzymes when the concentration of substrate is much greater than the concentration of enzyme. In this paper, Michaelis-Menten kinetics were used to describe the degradation of GFP, or, more accurately, the activity of the protease responsible for the degradation of GFP.
-Prior to this paper's publication, the degradation of GFP had been calculated using first-order kinetics. In that case, the degradation of GFP (whether it was fluorescing or not) would have been degraded at a constant rate, and would degrade faster the more protein was present. In theory, the degradation rate could increase indefinitely, so long as the amount of protein continued to increase. In a Michaelis-Menten equation, there is a maximum rate of degradation that an enzyme approaches as the concentration of substrate increases; however, it never ''Italic text''reaches''Italic text'' this maximum rate. So, after a certain concentration of GFP is reached, the degradation rate remains almost constant no matter how great the concentration of GFP. This can affect how much fluorescence is perceived in a cell and whether it corresponds to promoter activity.
+Prior to this paper's publication, the degradation of GFP had been calculated using first-order kinetics. In that case, the degradation of GFP (whether it was fluorescing or not) would have been degraded at a constant rate, and would degrade faster the more protein was present. In theory, the degradation rate could increase indefinitely, so long as the amount of protein continued to increase. In a Michaelis-Menten equation, there is a maximum rate of degradation that an enzyme approaches as the concentration of substrate increases; however, it never ''reaches'' this maximum rate. So, after a certain concentration of GFP is reached, the degradation rate remains almost constant no matter how great the concentration of GFP. This can affect how much fluorescence is perceived in a cell and whether it corresponds to promoter activity.
 == Class Wiki Project ==
 My class project will be on the design of temperature-sensing cellular mechanisms, such as cellular thermometers.

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 *[http://en.wikipedia.org/wiki/Michaelis-Menten_kinetics Michaelis-Menten Kinetics] is a system of equations that describes the kinetics of enzymes when the concentration of substrate is much greater than the concentration of enzyme. In this paper, Michaelis-Menten kinetics were used to describe the degradation of GFP, or, more accurately, the activity of the protease responsible for the degradation of GFP.
 == Class Wiki Project ==
 My class project will be on the design of temperature-sensing cellular mechanisms, such as cellular thermometers.

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 === Links for "Relative Sensitivities of Various Reporter Genes" ===
-*[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&id=124117 Ice Nucleation Proteins and inaZ]. InaZ is a promoter for ice nucleation proteins, which, according to NCBI, "enable bacteria to nucleate crystallation in supercooled water". Since supercooled water is chilled below its freezing point, ice nucleation proteins form the "seed" around which ice crystals can form in the cell. The more proteins are produced, the more ice crystals will be present in a cell. [http://en.wikipedia.org/wiki/Supercooling See the Wikipedia entry on supercooling.]
+*[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&id=124117 Ice Nucleation Proteins and inaZ]. InaZ is a reporter for ice nucleation proteins, which, according to NCBI, "enable bacteria to nucleate crystallation in supercooled water". Since supercooled water is chilled below its freezing point, ice nucleation proteins form the "seed" around which ice crystals can form in the cell. The more proteins are produced, the more ice crystals will be present in a cell. [http://en.wikipedia.org/wiki/Supercooling See the Wikipedia entry on supercooling.]
 === Links for "Simulation of GFP Expression" ===
-*[
 == Class Wiki Project ==
 My class project will be on the design of temperature-sensing cellular mechanisms, such as cellular thermometers.

← Older revision		Revision as of 05:37, 18 October 2007
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−	== ~~Laura Voss - Synthetic Biology Seminar~~ ==	+	== Paper Discussion 10/23 ==
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		+	== Class Wiki Project ==

	My class project will be on the design of temperature-sensing cellular mechanisms, such as cellular thermometers.		My class project will be on the design of temperature-sensing cellular mechanisms, such as cellular thermometers.