Kcochran3: Created page with "1) Get melamine deaminase in correct location in the plasmid. 2) Retest riboswitches with the biosensor. 3) Test riboswitches in ThyA fitness module (insert ThyA- into plasm..."

2014-05-21T18:39:27Z

Created page with "1) Get melamine deaminase in correct location in the plasmid. 2) Retest riboswitches with the biosensor. 3) Test riboswitches in ThyA fitness module (insert ThyA- into plasm..."

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1) Get melamine deaminase in correct location in the plasmid.

2) Retest riboswitches with the biosensor.

3) Test riboswitches in ThyA fitness module (insert ThyA- into plasmid).

4) Find a way to better dissolve ammeline (e.g. instead of toxic NaOH, use DMSO).

5) Research for more riboswitch options.

6) Use UNAFold and futher study of Dixon article to create new riboswitches.

7) Use pLac promoter instead of P5.

8) Put it all together: melamine deaminase, riboswitch, etc. This allows for the testing of different promoters and ribosome binding sites to optimize the fitness module.

MWSU To Do - Revision history

Kcochran3: Created page with "1) Get melamine deaminase in correct location in the plasmid. 2) Retest riboswitches with the biosensor. 3) Test riboswitches in ThyA fitness module (insert ThyA- into plasm..."