NHE Jan 14 Notes - Revision history

Nielder at 19:15, 14 January 2016

2016-01-14T19:15:58Z

Nielder at 19:14, 14 January 2016

2016-01-14T19:14:45Z

Nielder at 19:14, 14 January 2016

2016-01-14T19:14:29Z

Nielder at 19:13, 14 January 2016

2016-01-14T19:13:43Z

Nielder: Created page with "Nick Elder Group 2 intestines"

2016-01-14T18:37:49Z

Created page with "Nick Elder Group 2 intestines"

New page

[[Nick Elder]]
[[Group 2 intestines]]

← Older revision		Revision as of 19:15, 14 January 2016
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	Glucose transporter SGLT1		Glucose transporter SGLT1
		+
		+	How do microvilli grow? Cell division? Cell growth?

@@ Line 12: / Line 12: @@
 We need to find references to specific genes that would be active in liver or intestine, especially after feeding.
-  *Tolozoa, Lam, and Diamond
+*Tolozoa, Lam, and Diamond
-  *Karasov and Diamond
+*Karasov and Diamond
 We don't know when they started the clock, but either way we are most interested in the earliest time points
@@ Line 20: / Line 20: @@
 Amino acid absorption peaks early on in digestion. So does sugar.
- *recovery for having to double mass of organs?
+*recovery for having to double mass of organs?
- *transport mechanisms are produced early in digestion
+*transport mechanisms are produced early in digestion
- *most absorption happens in anterior small intestine
+*most absorption happens in anterior small intestine
 Is there an increase in transcription? Or are there post-translational modification (phosphorylation for example) that increase activity. Could also be increased translation.

@@ Line 12: / Line 12: @@
 We need to find references to specific genes that would be active in liver or intestine, especially after feeding.
-   Tolozoa, Lam, and Diamond
+   *Tolozoa, Lam, and Diamond
-   Karasov and Diamond
+   *Karasov and Diamond
 We don't know when they started the clock, but either way we are most interested in the earliest time points
@@ Line 20: / Line 20: @@
 Amino acid absorption peaks early on in digestion. So does sugar.
-  - recovery for having to double mass of organs?
+  *recovery for having to double mass of organs?
-  - transport mechanisms are produced early in digestion
+  *transport mechanisms are produced early in digestion
-  - most absorption happens in anterior small intestine
+  *most absorption happens in anterior small intestine
 Is there an increase in transcription? Or are there post-translational modification (phosphorylation for example) that increase activity. Could also be increased translation.

← Older revision		Revision as of 19:13, 14 January 2016
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	[[Nick Elder]]		[[Nick Elder]]
		+
	[[Group 2 intestines]]		[[Group 2 intestines]]
		+
		+	[[http://www.bio.davidson.edu/courses/Bio343/2016/1a_pay_B4_pumping_1995.pdf]]
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		+	[[http://www.bio.davidson.edu/courses/Bio343/2016/1b_physiology_feeding_1998.pdf]]
		+
		+	Intestine quickly doubles in size (paper looked at 1 to 3 days, but we are look at minutes to hours after feeding)
		+
		+	*Intestinal nutrient transporters and hydrolytic enzymes
		+
		+	We need to find references to specific genes that would be active in liver or intestine, especially after feeding.
		+	Tolozoa, Lam, and Diamond
		+	Karasov and Diamond
		+
		+	We don't know when they started the clock, but either way we are most interested in the earliest time points
		+
		+	Food that reaches intestine is mostly liquefied, so intestine is mostly absorption, not digestion
		+
		+	Amino acid absorption peaks early on in digestion. So does sugar.
		+	- recovery for having to double mass of organs?
		+	- transport mechanisms are produced early in digestion
		+	- most absorption happens in anterior small intestine
		+
		+	Is there an increase in transcription? Or are there post-translational modification (phosphorylation for example) that increase activity. Could also be increased translation.
		+	Only factor we can detect is transcription
		+
		+	Find intestinal transport proteins and homologs for other species.
		+
		+	*Burmese python genome is transcribed
		+
		+	serosa layer - outer layer that cover the membrane walls
		+	Most of organ growth occurs in the brush border in the intestine. (Possible inward growth by microvilli into intestine, not doubling of outer wall.)
		+
		+	When organs were frozen, cold not tell distal from anterior. Do not know if only serosa was sampled. Need microvilli if want the transport proteins RNA's to be included.
		+
		+	Some transporters drive amino acid uptake by ion gradients (sodium dependent/independent)
		+	Ion could enter with the aa or against aa
		+	From Fig 5, mostly look like sodium independent transport
		+
		+	Fig 7, ions, cholesterol, protein, phosphatase, etc...all enter the blood somehow - there must be transporters
		+
		+	Fig 9, most of growth in mass in small intestine is in anterior
		+
		+	Second Paper - Review paper - Python advertisement
		+
		+	Phylogenic comparison
		+	Noted microvilli increase mass
		+
		+	Glucose transporter SGLT1