PMnt/Lac - Revision history

AlAllen at 20:09, 30 June 2008

2008-06-30T20:09:04Z

AlAllen at 20:05, 30 June 2008

2008-06-30T20:05:50Z

AlAllen at 19:56, 30 June 2008

2008-06-30T19:56:20Z

AlAllen at 19:55, 30 June 2008

2008-06-30T19:55:47Z

AlAllen at 19:39, 30 June 2008

2008-06-30T19:39:08Z

AlAllen at 19:21, 30 June 2008

2008-06-30T19:21:50Z

New page

pMnt/Lac is constitutively on but is repressed by the LacI protein. The promoter is also repressed by the Mnt protein. The addition of IPTG should repress the LacI repressor and glow in the absence of the Mnt protein.

Some promoter→LacI+t+t+pLac/Mnt→GFP does not glow

Some promoter→Mnt+t+t+pLac/Mnt→GFP does not glow

add IPTG

Some promoter→LacI+t+t+pLac/Mnt→GFP glows

Some promoter→LacI+Mnt+t+t+pLac/Mnt→GFP does not glow

{| border="1" cellpadding="2"
!width="30"|LacI
!width="30"|Mnt
!width="30"|IPTG + GFP
!width="30"|IPTG - GFP
|-
| 0 || 0 || 1 || 1
|-
| 0 || 1 || 0 || 0
|-
| 1 || 0 || 1 || 0
|-
| 1 || 1 || 0 || 0
|}

@@ Line 1: / Line 1: @@
 pMnt/Lac is constitutively on but is repressed by the LacI protein. The promoter is also repressed by the Mnt protein. The addition of IPTG should repress the LacI repressor and glow in the absence of the Mnt protein.
-Plan to Test the promoter
+'''Plan to Test the promoter'''
-To Test repression using LacI
+'''To Test repression using LacI'''
 Some promoter→LacI+t+t+pLac/Mnt→GFP    does not glow
-To Test repression using Mnt protein
+'''To Test repression using Mnt protein'''
 Some promoter→Mnt+t+t+pLac/Mnt→GFP    does not glow
-To Test pLac being on with addition of IPTG
+'''To Test pLac being on with addition of IPTG'''
 add IPTG
@@ Line 21: / Line 21: @@
-To Test the repression of pLac
+'''To Test the repression of pLac'''
 Some promoter→LacI+Mnt+t+t+pLac/Mnt→GFP    does not glow
@@ Line 45: / Line 45: @@
--30-08 Ligation
+'''6-30-08 Ligation'''
 Mnt/ Lac [http://partsregistry.org/Part:BBa_K091104 K091104] was ligated to [http://partsregistry.org/Part:BBa_E0240 E0240]
 The plasmid + vector was then transformed onto an amp plate.

← Older revision		Revision as of 20:05, 30 June 2008
Line 41:		Line 41:
	\| 1 \|\| 1 \|\| 0 \|\| 0		\| 1 \|\| 1 \|\| 0 \|\| 0
	\|}		\|}
		+
		+
		+
		+
		+	6-30-08 Ligation
		+
		+	Mnt/ Lac [http://partsregistry.org/Part:BBa_K091104 K091104] was ligated to [http://partsregistry.org/Part:BBa_E0240 E0240]
		+
		+	The plasmid + vector was then transformed onto an amp plate.

@@ Line 7: / Line 7: @@
 Some promoter→LacI+t+t+pLac/Mnt→GFP    does not glow
 To Test repression using Mnt protein
 Some promoter→Mnt+t+t+pLac/Mnt→GFP    does not glow
 To Test pLac being on with addition of IPTG
@@ Line 17: / Line 19: @@
 Some promoter→LacI+t+t+pLac/Mnt→GFP     glows
 To Test the repression of pLac

← Older revision		Revision as of 19:39, 30 June 2008
Line 1:		Line 1:
	pMnt/Lac is constitutively on but is repressed by the LacI protein. The promoter is also repressed by the Mnt protein. The addition of IPTG should repress the LacI repressor and glow in the absence of the Mnt protein.		pMnt/Lac is constitutively on but is repressed by the LacI protein. The promoter is also repressed by the Mnt protein. The addition of IPTG should repress the LacI repressor and glow in the absence of the Mnt protein.
		+
		+	Plan to Test the promoter

	Some promoter→LacI+t+t+pLac/Mnt→GFP does not glow		Some promoter→LacI+t+t+pLac/Mnt→GFP does not glow