https://gcat.davidson.edu/GcatWiki/index.php?action=history&feed=atom&title=Phi80_Integration_Protocol 2026-05-17T07:53:46Z Revision history for this page on the wiki MediaWiki 1.28.2 https://gcat.davidson.edu/GcatWiki/index.php?title=Phi80_Integration_Protocol&diff=6444&oldid=prev 2008-09-18T18:07:46Z

<p></p> <table class="diff diff-contentalign-left" data-mw="interface"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr style='vertical-align: top;' lang='en'> <td colspan='2' style="background-color: white; color:black; text-align: center;">← Older revision</td> <td colspan='2' style="background-color: white; color:black; text-align: center;">Revision as of 18:07, 18 September 2008</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l8" >Line 8:</td> <td colspan="2" class="diff-lineno">Line 8:</td></tr> <tr><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>*swab the plate and outgrow in 5mL LB+gentamicin to saturation</div></td><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>*swab the plate and outgrow in 5mL LB+gentamicin to saturation</div></td></tr> <tr><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>*restreak on LB+gentamamicin at 37 degrees</div></td><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>*restreak on LB+gentamamicin at 37 degrees</div></td></tr> <tr><td class='diff-marker'>−</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del class="diffchange diffchange-inline">(Taken </del>from Anderson Lab, UC Berkeley<del class="diffchange diffchange-inline">)</del></div></td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins class="diffchange diffchange-inline">Note: Protocol adapted </ins>from Anderson Lab, UC Berkeley</div></td></tr> </table>

Wideloache https://gcat.davidson.edu/GcatWiki/index.php?title=Phi80_Integration_Protocol&diff=6443&oldid=prev 2008-09-18T18:07:17Z

<p></p> <table class="diff diff-contentalign-left" data-mw="interface"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr style='vertical-align: top;' lang='en'> <td colspan='2' style="background-color: white; color:black; text-align: center;">← Older revision</td> <td colspan='2' style="background-color: white; color:black; text-align: center;">Revision as of 18:07, 18 September 2008</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l8" >Line 8:</td> <td colspan="2" class="diff-lineno">Line 8:</td></tr> <tr><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>*swab the plate and outgrow in 5mL LB+gentamicin to saturation</div></td><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>*swab the plate and outgrow in 5mL LB+gentamicin to saturation</div></td></tr> <tr><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>*restreak on LB+gentamamicin at 37 degrees</div></td><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>*restreak on LB+gentamamicin at 37 degrees</div></td></tr> <tr><td colspan="2">&#160;</td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">(Taken from Anderson Lab, UC Berkeley)</ins></div></td></tr> </table>

Wideloache https://gcat.davidson.edu/GcatWiki/index.php?title=Phi80_Integration_Protocol&diff=6440&oldid=prev 2008-09-18T18:06:22Z

<p>Phi80 protocol moved to Phi80 Integration Protocol</p> <table class="diff diff-contentalign-left" data-mw="interface"> <tr style='vertical-align: top;' lang='en'> <td colspan='1' style="background-color: white; color:black; text-align: center;">← Older revision</td> <td colspan='1' style="background-color: white; color:black; text-align: center;">Revision as of 18:06, 18 September 2008</td> </tr><tr><td colspan='2' style='text-align: center;' lang='en'><div class="mw-diff-empty">(No difference)</div> </td></tr></table>

Wideloache https://gcat.davidson.edu/GcatWiki/index.php?title=Phi80_Integration_Protocol&diff=6439&oldid=prev 2008-09-18T18:05:21Z

<p></p> <p><b>New page</b></p><div>*Prepare chemically competent cells of the strain you want to integrate into<br /> *Transform in pInt80-649, plate on amp at 30 degrees<br /> *Pick a single colony, grow a starter ON<br /> *Dilute 200uL to 5mL, grow at 30 degree to mid log<br /> *Do another chemically competent cell prep<br /> *Transform in the phi80 CRIM plasmid you wish to integrate<br /> *Rescue 1 hr in LB, plate on gentamicin at 37<br /> *swab the plate and outgrow in 5mL LB+gentamicin to saturation<br /> *restreak on LB+gentamamicin at 37 degrees</div>

Wideloache