Prepare PCR product for sequencing - Revision history

Macampbell at 20:15, 9 October 2013

2013-10-09T20:15:35Z

Macampbell at 03:03, 11 October 2012

2012-10-11T03:03:09Z

Macampbell at 15:51, 9 October 2012

2012-10-09T15:51:42Z

Macampbell at 18:54, 4 October 2012

2012-10-04T18:54:41Z

Macampbell at 14:56, 7 August 2012

2012-08-07T14:56:24Z

Macampbell: Created page with 'You have just cleaned and concentrated your DNA for sequencing. Now you need to mix the sequencing primer with your PCR product. # Pipet 5 µL of clean PCR product into the bar…'

2012-07-06T21:43:07Z

Created page with 'You have just cleaned and concentrated your DNA for sequencing. Now you need to mix the sequencing primer with your PCR product. # Pipet 5 µL of clean PCR product into the bar…'

New page

You have just cleaned and concentrated your DNA for sequencing. Now you need to mix the sequencing primer with your PCR product.

# Pipet 5 µL of clean PCR product into the bar coded tube.
# Pipet 9 µL of sequencing primer into the same bar coded tube.
# Place the small cap back onto the bar coded sequencing tube.
# Record the bar code number of your tube. Write your name and bar code number on the sheet in the lab.

@@ Line 4: / Line 4: @@
 # Pipet 4 µL of sequencing primer (at 6 µM) into the same bar coded tube.
 # Place the small cap back onto the bar coded sequencing tube.
-# Record the bar code number of your tube. Write your name and bar code number on the sheet in the lab.
+# Record the bar code number of your tube. Write your name next to the bar code number on the sheet in lab.
 # The information will be submitted to the sequencing company [http://www.operon.com/default.aspx MWG Operon].

← Older revision		Revision as of 03:03, 11 October 2012
Line 1:		Line 1:
−	You have ~~just cleaned and concentrated~~ your ~~DNA~~ for sequencing. Now you need to mix the sequencing primer with your PCR product.	+	You have amplified your TAS2R38 alleles for sequencing. Now you need to mix the sequencing primer with your PCR product.

	# Pipet 8 µL of PCR product into the bar coded tube.		# Pipet 8 µL of PCR product into the bar coded tube.

@@ Line 2: / Line 2: @@
 # Pipet 8 µL of PCR product into the bar coded tube.
-# Pipet 4 µL of sequencing primer into the same bar coded tube.
+# Pipet 4 µL of sequencing primer (at 6 µM) into the same bar coded tube.
 # Place the small cap back onto the bar coded sequencing tube.
 # Record the bar code number of your tube. Write your name and bar code number on the sheet in the lab.
 # The information will be submitted to the sequencing company [http://www.operon.com/default.aspx MWG Operon].

@@ Line 1: / Line 1: @@
 You have just cleaned and concentrated your DNA for sequencing. Now you need to mix the sequencing primer with your PCR product.
-# Pipet 8 µL of clean PCR product into the bar coded tube.
+# Pipet 8 µL of PCR product into the bar coded tube.
 # Pipet 4 µL of sequencing primer into the same bar coded tube.
 # Place the small cap back onto the bar coded sequencing tube.
 # Record the bar code number of your tube. Write your name and bar code number on the sheet in the lab.