Previous iGEM projects - Revision history

Dwagner5 at 00:18, 16 April 2010

2010-04-16T00:18:17Z

2010-04-16T00:17:56Z

2010-04-16T00:11:36Z

2010-04-16T00:10:38Z

2010-04-16T00:10:22Z

2010-04-16T00:04:30Z

2010-04-15T04:11:47Z

2010-04-09T20:56:24Z

2010-04-09T20:44:56Z

2010-04-09T20:37:29Z

← Older revision		Revision as of 00:18, 16 April 2010
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	[http://2009.igem.org/Team:Newcastle/Stochasticity Newcastle iGEM 2009]		[http://2009.igem.org/Team:Newcastle/Stochasticity Newcastle iGEM 2009]
−
−
	"Stochastic Switch"		"Stochastic Switch"

@@ Line 15: / Line 15: @@
        probability of loop formation. "
-[http://2009.igem.org/Team:Newcastle/Stochasticity]
+[http://2009.igem.org/Team:Newcastle/Stochasticity Newcastle iGEM 2009]
 "Stochastic Switch"
@@ Line 23: / Line 22: @@
 *attempted to create a synthetic stochastic switch that would decide whether it wanted to be a metal-containing spore or a spore that goes on to germinate as in the normal cell cycle.
 *Used an invertible piece of DNA that had promoters on either side so depending on the orientation of the DNA, coding sequences would be expressed based on the decision of whether to be a metal container or not.
-*Used Metlab to utilize the Gillespie algorithm [http://en.wikipedia.org/wiki/Gillespie_algorithm] for incorporating noise in cells.
+*Used Metlab to utilize the [http://en.wikipedia.org/wiki/Gillespie_algorithm Gillespie algorithm] for incorporating noise in cells.

@@ Line 23: / Line 23: @@
 *attempted to create a synthetic stochastic switch that would decide whether it wanted to be a metal-containing spore or a spore that goes on to germinate as in the normal cell cycle.
 *Used an invertible piece of DNA that had promoters on either side so depending on the orientation of the DNA, coding sequences would be expressed based on the decision of whether to be a metal container or not.
-*Used Metlab to utilize the Gillespie algorithm for incorporating noise in cells.
+*Used Metlab to utilize the Gillespie algorithm [http://en.wikipedia.org/wiki/Gillespie_algorithm] for incorporating noise in cells.

@@ Line 23: / Line 23: @@
 *attempted to create a synthetic stochastic switch that would decide whether it wanted to be a metal-containing spore or a spore that goes on to germinate as in the normal cell cycle.
 *Used an invertible piece of DNA that had promoters on either side so depending on the orientation of the DNA, coding sequences would be expressed based on the decision of whether to be a metal container or not.
-*Used Metlab to use the Gillespie algorithm for incorporating noise in cells.
+*Used Metlab to utilize the Gillespie algorithm for incorporating noise in cells.

← Older revision		Revision as of 00:10, 16 April 2010
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	*attempted to create a synthetic stochastic switch that would decide whether it wanted to be a metal-containing spore or a spore that goes on to germinate as in the normal cell cycle.		*attempted to create a synthetic stochastic switch that would decide whether it wanted to be a metal-containing spore or a spore that goes on to germinate as in the normal cell cycle.
	*Used an invertible piece of DNA that had promoters on either side so depending on the orientation of the DNA, coding sequences would be expressed based on the decision of whether to be a metal container or not.		*Used an invertible piece of DNA that had promoters on either side so depending on the orientation of the DNA, coding sequences would be expressed based on the decision of whether to be a metal container or not.
		+	*Used Metlab to use the Gillespie algorithm for incorporating noise in cells.

← Older revision		Revision as of 00:04, 16 April 2010
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	* Then, can we tune the probability (from, say, 60:40 to 80:20 to 20:80)? Ideally do this dynamically (based on some small molecule) - use proteins that bend DNA to affect the		* Then, can we tune the probability (from, say, 60:40 to 80:20 to 20:80)? Ideally do this dynamically (based on some small molecule) - use proteins that bend DNA to affect the
	probability of loop formation. "		probability of loop formation. "
		+
		+	[http://2009.igem.org/Team:Newcastle/Stochasticity]
		+
		+	Newcastle iGEM 2009
		+
		+	"Stochastic Switch"
		+
		+	*attempted to create a synthetic stochastic switch that would decide whether it wanted to be a metal-containing spore or a spore that goes on to germinate as in the normal cell cycle.
		+	*Used an invertible piece of DNA that had promoters on either side so depending on the orientation of the DNA, coding sequences would be expressed based on the decision of whether to be a metal container or not.

← Older revision		Revision as of 04:11, 15 April 2010
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	[http://parts.mit.edu/igem07/index.php/Davidson_Missouri_W Davidson & MWSU 2007] Hin/Hix random flipping		[http://parts.mit.edu/igem07/index.php/Davidson_Missouri_W Davidson & MWSU 2007] Hin/Hix random flipping
		+
		+	Found a small snippet from the iGEM Idea Exchange in which the MIT Synthetic Biology Working Group listed desired parts that weren't in the registry as of 2008. [http://openwetware.org/wiki/IGEM:Idea_exchange Idea Exchange]
		+
		+	" Random Number Generator
		+
		+	* FimE inverts a specific stretch of DNA, defined by a pair of sequence elements (IRR and IRL), forming a DNA loop between the two elements[3]. If we add multiple copies of one
		+	of these elements (one IRR, two IRL), would FimE randomly choose one of the sites (one IRL out of the pair) to invert between? Either choose one of several promoters to attach
		+	to a given gene, or one of several genes to attach to a given promoter.
		+	* Then, can we tune the probability (from, say, 60:40 to 80:20 to 20:80)? Ideally do this dynamically (based on some small molecule) - use proteins that bend DNA to affect the
		+	probability of loop formation. "

← Older revision		Revision as of 20:56, 9 April 2010
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	[http://2008.igem.org/Team:HKUSTers Hong Kong HKUST 2008]		[http://2008.igem.org/Team:HKUSTers Hong Kong HKUST 2008]
		+
		+	This team tried to create a randomizer that chooses between two overlapping promoters that are mutually exclusive. One produces RFP while the other produces GFP. The team has a simulation and submitted parts to the registry but we were unable to find any conclusive results.

	[http://parts.mit.edu/igem07/index.php/Davidson_Missouri_W Davidson & MWSU 2007] Hin/Hix random flipping		[http://parts.mit.edu/igem07/index.php/Davidson_Missouri_W Davidson & MWSU 2007] Hin/Hix random flipping

← Older revision		Revision as of 20:44, 9 April 2010
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−	[http://2008.igem.org/Team:HKUSTers Hong Kong HKUST]	+	[http://2008.igem.org/Team:HKUSTers Hong Kong HKUST 2008]
		+
		+	[http://parts.mit.edu/igem07/index.php/Davidson_Missouri_W Davidson & MWSU 2007] Hin/Hix random flipping

← Older revision		Revision as of 20:37, 9 April 2010
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−	http://2008.igem.org/Team:HKUSTers	+	[http://2008.igem.org/Team:HKUSTers Hong Kong HKUST]