Repeating Eckdahl's 20-clone selection - Revision history

Mospencer at 18:38, 4 June 2014

2014-06-04T18:38:39Z

Mospencer at 18:33, 4 June 2014

2014-06-04T18:33:39Z

Jolim at 20:01, 31 May 2014

2014-05-31T20:01:26Z

Jolim at 17:22, 31 May 2014

2014-05-31T17:22:32Z

Jolim at 19:28, 29 May 2014

2014-05-29T19:28:49Z

Jolim: Jolim moved page Repeating 20 Clone Experiments to Repeating Eckdahl's 20-clone selection

2014-05-21T15:01:18Z

Jolim moved page Repeating 20 Clone Experiments to Repeating Eckdahl's 20-clone selection

Jolim: Description and index of 24-clone replication project

2014-05-21T15:00:34Z

Description and index of 24-clone replication project

Macampbell: Created page with "We need to replicate Dr. Eckdahl’s 20-clone experiment twice on both campuses and mathematically analyze the data. Continue adding arabinose to induce the chaperones. We ne..."

2014-05-21T11:58:37Z

Created page with "We need to replicate Dr. Eckdahl’s 20-clone experiment twice on both campuses and mathematically analyze the data. Continue adding arabinose to induce the chaperones. We ne..."

New page

We need to replicate Dr. Eckdahl’s 20-clone experiment twice on both campuses and mathematically analyze the data. Continue adding arabinose to induce the chaperones.

We need to grow the surviving clones on individual plates: no antibiotic, amp, amp+chlor. This will enable us to determine whether both plasmids (CDM+amp, chaperone+chlor) are still present and being transcribed.

Depending on the survivorship of the clones in the previous step, set up plates to run head-to-head competition between two surviving clones, and determine which strain is most competent. This will confirm the results of the 20-clone experiment, in a more controlled environment than a plate with all 20 clones.

← Older revision		Revision as of 18:38, 4 June 2014
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	Template for results: [[File:Replication Results Template.xlsx]]		Template for results: [[File:Replication Results Template.xlsx]]
−	Upload a file for ~~both campuses~~ with the results displayed in the table. There is a tab in the file for each trial.	+	Upload a file for each campus with the results displayed in the table. There is a tab in the file for each trial.

← Older revision		Revision as of 18:33, 4 June 2014
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	[https://www.dropbox.com/sh/2jqkqfz755ng0vc/AAA26M9kEeYii0pFtFlCQ17na Photos from Davidson's first replication (four plates)] (Updated May 31, 2014)		[https://www.dropbox.com/sh/2jqkqfz755ng0vc/AAA26M9kEeYii0pFtFlCQ17na Photos from Davidson's first replication (four plates)] (Updated May 31, 2014)
		+
		+	Template for results: [[File:Replication Results Template.xlsx]]
		+	Upload a file for both campuses with the results displayed in the table. There is a tab in the file for each trial.

← Older revision		Revision as of 20:01, 31 May 2014
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	Protocol created on 05/21/14: [[Eckdahl-replication-protocol]]		Protocol created on 05/21/14: [[Eckdahl-replication-protocol]]

−	[https://www.dropbox.com/sh/2jqkqfz755ng0vc/AAA26M9kEeYii0pFtFlCQ17na Photos from Davidson's first replication (four plates)] (Updated May 30, 2014)	+	[https://www.dropbox.com/sh/2jqkqfz755ng0vc/AAA26M9kEeYii0pFtFlCQ17na Photos from Davidson's first replication (four plates)] (Updated May 31, 2014)

← Older revision		Revision as of 17:22, 31 May 2014
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	Protocol created on 05/21/14: [[Eckdahl-replication-protocol]]		Protocol created on 05/21/14: [[Eckdahl-replication-protocol]]

−	[https://www.dropbox.com/sh/2jqkqfz755ng0vc/AAA26M9kEeYii0pFtFlCQ17na Photos from Davidson's first replication (four plates)]	+	[https://www.dropbox.com/sh/2jqkqfz755ng0vc/AAA26M9kEeYii0pFtFlCQ17na Photos from Davidson's first replication (four plates)] (Updated May 30, 2014)

← Older revision		Revision as of 19:28, 29 May 2014
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	Protocol created on 05/21/14: [[Eckdahl-replication-protocol]]		Protocol created on 05/21/14: [[Eckdahl-replication-protocol]]
		+
		+	[https://www.dropbox.com/sh/2jqkqfz755ng0vc/AAA26M9kEeYii0pFtFlCQ17na Photos from Davidson's first replication (four plates)]

← Older revision	Revision as of 15:01, 21 May 2014
(No difference)

← Older revision		Revision as of 15:00, 21 May 2014
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	Depending on the survivorship of the clones in the previous step, set up plates to run head-to-head competition between two surviving clones, and determine which strain is most competent. This will confirm the results of the 20-clone experiment, in a more controlled environment than a plate with all 20 clones.		Depending on the survivorship of the clones in the previous step, set up plates to run head-to-head competition between two surviving clones, and determine which strain is most competent. This will confirm the results of the 20-clone experiment, in a more controlled environment than a plate with all 20 clones.
		+
		+	Protocol created on 05/21/14: [[Eckdahl-replication-protocol]]