https://gcat.davidson.edu/GcatWiki/index.php?action=history&feed=atom&title=SOC_Protocol_for_Transformations 2026-05-17T15:07:49Z Revision history for this page on the wiki MediaWiki 1.28.2 https://gcat.davidson.edu/GcatWiki/index.php?title=SOC_Protocol_for_Transformations&diff=17542&oldid=prev 2014-07-03T19:18:50Z

<p></p> <table class="diff diff-contentalign-left" data-mw="interface"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr style='vertical-align: top;' lang='en'> <td colspan='2' style="background-color: white; color:black; text-align: center;">← Older revision</td> <td colspan='2' style="background-color: white; color:black; text-align: center;">Revision as of 19:18, 3 July 2014</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1" >Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'>−</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del class="diffchange diffchange-inline">'''</del>'''Transformation Protocol'''</div></td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>'''Transformation Protocol'''</div></td></tr> <tr><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr> <tr><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>1. Combine GGA (Golden Gate Assembly) products then redistribute 10 uL of GGA product into PCR tubes.</div></td><td class='diff-marker'>&#160;</td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>1. Combine GGA (Golden Gate Assembly) products then redistribute 10 uL of GGA product into PCR tubes.</div></td></tr> </table>

Mquaney https://gcat.davidson.edu/GcatWiki/index.php?title=SOC_Protocol_for_Transformations&diff=17541&oldid=prev 2014-07-03T19:18:31Z

<p>Created page with &quot;&#039;&#039;&#039;&#039;&#039;&#039;Transformation Protocol&#039;&#039;&#039; 1. Combine GGA (Golden Gate Assembly) products then redistribute 10 uL of GGA product into PCR tubes. 2. Add GGA product to 50 uL (whole tub...&quot;</p> <p><b>New page</b></p><div>''''''Transformation Protocol'''<br /> <br /> 1. Combine GGA (Golden Gate Assembly) products then redistribute 10 uL of GGA product into PCR tubes.<br /> <br /> 2. Add GGA product to 50 uL (whole tube) of competent cells.<br /> <br /> 3. Place tube in ice box for 5 minutes.<br /> <br /> 4. Meanwhile, put media plate faced down in the bottom shelf of the incubator.<br /> <br /> 5. Take tube out of ice box after 5 minutes and add 4 volumes of SOC broth (240 mL to 60 mL of cells, 300 mL total) to a 1.5 mL tube, add competent cells in the SOC 1.5 mL tube.<br /> <br /> 6. Incubate the tubes in a foam rack (sideways in a beaker) in the shaker for 1 hour.<br /> <br /> 7. After 1 hour, take tubes and spin in centrifuge for 1 minute.<br /> <br /> 8. Pipette off 250 uL of supernatent and discard.<br /> <br /> 9. Mix pellet and approximately 50 uL of remaining supernatent.<br /> <br /> 10. Plate whole (approx. 50 uL) tube on LB + Amp plate.<br /> <br /> (if transforming cells in broth, stop after step 6, place all 300 uL of SOC broth and GGA product into the aliquotted tube (8 mL for single, 16 mL for double)</div>

Mquaney