Sequencing at Agencourt - Revision history

Macampbell: /* Quicklane Sequencing Service */

2010-03-22T14:13:52Z

‎Quicklane Sequencing Service

Macampbell: /* Quicklane Sequencing Service */

2010-03-22T14:13:14Z

‎Quicklane Sequencing Service

Macampbell: /* Quicklane Sequencing Service */

2010-03-22T14:12:56Z

‎Quicklane Sequencing Service

Macampbell: /* Quicklane Sequencing Service */

2010-03-22T14:10:59Z

‎Quicklane Sequencing Service

Macampbell: /* Quicklane Sequencing Service */

2010-03-22T14:10:47Z

‎Quicklane Sequencing Service

Macampbell: /* Quicklane Sequencing Service */

2010-03-22T14:10:33Z

‎Quicklane Sequencing Service

Macampbell at 03:27, 3 March 2010

2010-03-03T03:27:23Z

Macampbell at 03:26, 3 March 2010

2010-03-03T03:26:55Z

Olhoshing at 15:09, 20 July 2009

2009-07-20T15:09:16Z

Kilau: /* Quicklane Sequencing Service */

2009-07-20T14:48:28Z

‎Quicklane Sequencing Service

@@ Line 16: / Line 16: @@
-'''Downloading Sequences'''
+'''Sample Submission'''
 *Click [http://www.agencourt.com/sample/ here] for sample submission. The sequencing account's username is the professor's full name and the password is the name of the organism studied in Biology111.
@@ Line 29: / Line 29: @@
 *Package the samples and use Standard Overnight FedEx shipment to mail to:  Beckman Coulter Genomics / Attention Genomic Services / 36 Cherry Hill Drive / Danvers, MA  01923 <br><br>
-'''Sample Submission'''
+'''Downloading Sequences'''
 Download our sequences from the secure server at https://cust.agencourt.com

← Older revision		Revision as of 14:13, 22 March 2010
Line 16:		Line 16:


−	'''~~Sample Submission~~'''	+	'''Downloading Sequences'''

	*Click [http://www.agencourt.com/sample/ here] for sample submission. The sequencing account's username is the professor's full name and the password is the name of the organism studied in Biology111.		*Click [http://www.agencourt.com/sample/ here] for sample submission. The sequencing account's username is the professor's full name and the password is the name of the organism studied in Biology111.

@@ Line 28: / Line 28: @@
 *Include Online Sample Submission and text file with well ID sample names with the samples to ship.
 *Package the samples and use Standard Overnight FedEx shipment to mail to:  Beckman Coulter Genomics / Attention Genomic Services / 36 Cherry Hill Drive / Danvers, MA  01923 <br><br>
 ==Sequencing Glycerol Cell Stocks==

@@ Line 27: / Line 27: @@
 *Pay with an acquired Purchase Order. The Service Quotation Number, if not already entered, is the one given by the college's sales representative (AGEN #####).
 *Include Online Sample Submission and text file with well ID sample names with the samples to ship.
-*Package the samples and use Standard Overnight FedEx shipment to mail to:  Beckman Coulter Genomics / Attention Genomic Services / 36 Cherry Hill Drive / Danvers, MA  01923 <br>
+*Package the samples and use Standard Overnight FedEx shipment to mail to:  Beckman Coulter Genomics / Attention Genomic Services / 36 Cherry Hill Drive / Danvers, MA  01923 <br><br>
 ==Sequencing Glycerol Cell Stocks==

@@ Line 27: / Line 27: @@
 *Pay with an acquired Purchase Order. The Service Quotation Number, if not already entered, is the one given by the college's sales representative (AGEN #####).
 *Include Online Sample Submission and text file with well ID sample names with the samples to ship.
-*Package the samples and use Standard Overnight FedEx shipment to mail to:  Beckman Coulter Genomics / Attention Genomic Services / 36 Cherry Hill Drive / Danvers, MA  01923
+*Package the samples and use Standard Overnight FedEx shipment to mail to:  Beckman Coulter Genomics / Attention Genomic Services / 36 Cherry Hill Drive / Danvers, MA  01923 <br>
 ==Sequencing Glycerol Cell Stocks==

@@ Line 1: / Line 1: @@
 ==Quicklane Sequencing Service==
-'''Be sure to use Internet Explorer only on a Windows machine. Otherwise, the Agencourt server can hang up and not work.
+'''Be sure to use Internet Explorer only on a Windows machine. Otherwise, the Agencourt server can hang up and not work. '''
-'''
+<br>
 Quicklane sequencing provides 24-hour turnaround so that you can receive sample sequences more quickly, for a higher price than slower sequencing services. Submitting miniprepped DNA samples with primers premixed is an available and recommended option. You can always find the complete [http://www.agencourt.com/documents/services/agencourt-sample-preparation-guidelines.pdf Sample Preparation] and [http://www.agencourt.com/documents/services/agencourt-sample-submission-guidelines.pdf Sample Submission] instructions on the Agencourt website.

← Older revision		Revision as of 03:26, 3 March 2010
Line 1:		Line 1:
	==Quicklane Sequencing Service==		==Quicklane Sequencing Service==

		+	'''Be sure to use Internet Explorer only on a Windows machine. Otherwise, the Agencourt server can hang up and not work.
		+	'''
	Quicklane sequencing provides 24-hour turnaround so that you can receive sample sequences more quickly, for a higher price than slower sequencing services. Submitting miniprepped DNA samples with primers premixed is an available and recommended option. You can always find the complete [http://www.agencourt.com/documents/services/agencourt-sample-preparation-guidelines.pdf Sample Preparation] and [http://www.agencourt.com/documents/services/agencourt-sample-submission-guidelines.pdf Sample Submission] instructions on the Agencourt website.		Quicklane sequencing provides 24-hour turnaround so that you can receive sample sequences more quickly, for a higher price than slower sequencing services. Submitting miniprepped DNA samples with primers premixed is an available and recommended option. You can always find the complete [http://www.agencourt.com/documents/services/agencourt-sample-preparation-guidelines.pdf Sample Preparation] and [http://www.agencourt.com/documents/services/agencourt-sample-submission-guidelines.pdf Sample Submission] instructions on the Agencourt website.

@@ Line 26: / Line 26: @@
 *Include Online Sample Submission and text file with well ID sample names with the samples to ship.
 *Package the samples and use Standard Overnight FedEx shipment to mail to:  Agencourt Bioscience Corp. / 500 Cummings Center, Suite 2450 / Attention Genomic Services / Beverly, MA 01915
 ==Sequencing Glycerol Cell Stocks==
@@ Line 31: / Line 33: @@
 SinglePass Plasmid sequencing service allows you to submit a bacterial culture that contains your plasmid to be sequenced, and Agencourt will do the miniprepping, purifying and sequencing for you. You can submit your own primers, or request they use one of their own universal primers for no extra charge. Expect sequence results 4 - 5 days after samples have been received.
-'''This sample preparation will require 12 hrs for incubating and growing your cultures. Plan your time wisely.''' You can always find the complete [http://www.agencourt.com/documents/services/agencourt-sample-preparation-guidelines.pdf Sample Preparation] and [http://www.agencourt.com/documents/services/agencourt-sample-submission-guidelines.pdf Sample Submission] instructions on the Agencourt website.
+'''This sample preparation will require 12 hrs for growing your cultures, and dry ice for sample shipment. Plan your preparation wisely.''' You can always find the complete [http://www.agencourt.com/documents/services/agencourt-sample-preparation-guidelines.pdf Sample Preparation] and [http://www.agencourt.com/documents/services/agencourt-sample-submission-guidelines.pdf Sample Submission] instructions on the Agencourt website.

@@ Line 7: / Line 7: @@
 *Prepare a 96-well plate or 2D barcoded tubes, provided by Agencourt. Label barcoded tubes with a distinct sample name. Label plates with a distinct name for your sequencing project.
-*You must submit 600 - 1000 ng of MP template DNA (mixed with primer) in a final volume of 40 ul for each sequencing reaction. It may be easiest to dry down 600 - 1000 ul DNA and resuspend. Try to submit roughly the same concentration for all samples (15 - 25 ng/ul).
+*You must submit 600 - 1000 ng of MP template DNA (mixed with primer) in a final volume of 40 ul for each sequencing reaction. It may be easiest to dry down 600 - 1000 ng DNA and resuspend. Try to submit roughly the same concentration for all samples (15 - 25 ng/ul).
 *Add 0.2 pmoles of primer for each sample (if you have 100 uM primer, just dilute it 10X and pipet 2 ul into each tube). If you are sequencing with forward and reverse primers, those are 2 different sequencing reactions, and two different tubes with 1 primer each.
 *Resuspend dried DNA and primer in nuclease-free water to a final volume of 40 ul.
@@ Line 26: / Line 26: @@
 *Include Online Sample Submission and text file with well ID sample names with the samples to ship.
 *Package the samples and use Standard Overnight FedEx shipment to mail to:  Agencourt Bioscience Corp. / 500 Cummings Center, Suite 2450 / Attention Genomic Services / Beverly, MA 01915
 ==Sequencing Glycerol Cell Stocks==