Sequencing at MWG Operon - Revision history

Macampbell: /* How to Submit SimpleSeq Samples */

2014-04-10T13:27:32Z

‎How to Submit SimpleSeq Samples

2014-04-10T13:26:54Z

‎How to Submit SimpleSeq Samples

2012-10-09T17:58:55Z

2010-06-21T21:13:54Z

2010-06-04T21:18:07Z

‎How to Submit SimpleSeq Samples

2010-06-04T18:27:44Z

‎How to Submit SimpleSeq Samples

2010-04-30T02:13:01Z

2010-04-30T02:12:27Z

2010-04-30T02:11:31Z

2010-04-30T02:10:52Z

@@ Line 28: / Line 28: @@
 * Make template and primer solutions that match the '''final concentration''' ranges listed below: <br>
 <center>[[Image:SimpleSeq.png]]</center>
-Catherine Doyle has found another way to get reasonable results with less DNA.
+Catherine Doyle has found another way to get reasonable results with less DNA. <br>
-I add 8µL of 40ng/µL template and 4µL of 2µM primer.
+'''I add 8µL of 40ng/µL template and 4µL of 2µM primer.''' <br>

@@ Line 28: / Line 28: @@
 * Make template and primer solutions that match the '''final concentration''' ranges listed below: <br>
 <center>[[Image:SimpleSeq.png]]</center>
 <br>
 Ship DNA sequencing template and primers to:<br>

@@ Line 26: / Line 26: @@
 * Mix 8 μL of template and 4 μL of primer for a total volume of 12 μL.
 * Place the order on line, write down the order number, then call customer service with the order number to make sure they remove the FedEx charge. (800) 688-2248
-* Make template and primer solutions that match the concentration ranges listed below: <br>
+* Make template and primer solutions that match the '''final concentration''' ranges listed below: <br>
 <center>[[Image:SimpleSeq.png]]</center>
 <br>

@@ Line 2: / Line 2: @@
 # Go to [http://www.operon.com www.operon.com] and log in. Username is Dr. C's full email address and the password is the nickname for the algae used in Bio111.
 # In the “Order Now” drop-down menu on the right-hand side, click “Order – DNA sequencing”.
-# If you already have your SimpleSeq tubes, click “Activate SimpleSeq Reactions”.
+# If you already have your SimpleSeq tubes and are the first person to use this set of 96 tubes, click “Activate SimpleSeq Reactions”. If you are using a set of 96 tubes after one or more has been used, then all you have to do is click on the radio button that says "tubes" and fill in the form to notify them that the tubes are coming. This is step #6 described below.
-# Enter either the barcode located on the side of the tube rack or a barcode from any tube in the rack. Click “Activate”.
+# If you have to activate, then enter either the barcode located on the side of the tube rack or a barcode from any tube in the rack. Click “Activate”.
 # Close out of that window.
 # Select your appropriate “Format” and “Sample Format” <br><center>'''In this lab, the “Format” is usually “Tubes” and the “Sample Format” is usually “Premix DNA Template and Primer”'''</center>

← Older revision		Revision as of 21:18, 4 June 2010
Line 29:		Line 29:
	<center>[[Image:SimpleSeq.png]]</center>		<center>[[Image:SimpleSeq.png]]</center>
	<br>		<br>
		+	Ship DNA sequencing template and primers to:<br>
		+	Eurofins MWG Operon <br>
		+	Attention: DNA Sequencing Lab <br>
		+	2211 Seminole Drive <br>
		+	Huntsville, AL 35805 <br>

@@ Line 7: / Line 7: @@
 # Select your appropriate “Format” and “Sample Format” <br><center>'''In this lab, the “Format” is usually “Tubes” and the “Sample Format” is usually “Premix DNA Template and Primer”'''</center>
 # For each DNA sample you’re submitting, enter:
---“Barcode”: the barcode number of the tube.
+--  “Barcode”: the barcode number of the tube.
---“Sample Name”: the name of your sample.<br>
+--  “Sample Name”: the name of your sample.<br>
---“Template Type”: select “Plasmid” if using miniprepped DNA.<br>
+--  “Template Type”: select “Plasmid” if using miniprepped DNA.<br>
---“Size (bp)”: the size of the whole vector including the insert. <br>
+--  “Size (bp)”: the size of the whole vector including the insert. <br>
 When you’re finished, click “Submit Samples”.<br>

@@ Line 19: / Line 19: @@
 # Select your shipping address and billing address and click “Next”.
 # Enter your payment information and click “Next”.<br><br>
-<center>'''In this lab, we pay with our PO number. Retrieve the PO number from Dr. Campbe'''ll.<center>
+<center>'''In this lab, we pay with our PO number. Retrieve the PO number from Dr. Campbell.'''</center>
 ===How to Submit SimpleSeq Samples===