Synergy Machine Protocol - Revision history

Spchadinha at 13:23, 13 June 2013

2013-06-13T13:23:34Z

2013-06-13T13:20:43Z

2012-07-06T20:15:46Z

2012-07-06T20:15:09Z

2012-07-06T20:14:59Z

2012-06-08T18:54:30Z

2012-06-07T19:48:48Z

2012-06-07T19:47:12Z

2012-06-07T19:04:45Z

2012-06-07T19:04:05Z

@@ Line 3: / Line 3: @@
 #Open Gen 5 software by double clicking on the desktop shortcut.
 #Click on Existing protocol OR move to step 4.
-#Click on Create New Protocol
+#Click on Create New Protocol. This option is available under "File", New Task.
 #Select Standard Protocol type
 #Click on Procedure

@@ Line 2: / Line 2: @@
 #All Wells should contain 200 microliters of cells in broth. Be sure to include wells containing broth with no cells- for negative control.
 #Open Gen 5 software by double clicking on the desktop shortcut.
-#Click on Existing protocol OR move to step 3
+#Click on Existing protocol OR move to step 4.
 #Click on Create New Protocol
 #Select Standard Protocol type

@@ Line 1: / Line 1: @@
 Synergy Machine Protocol
-#All Wells should contain 200 microliters of cells in broth.
+#All Wells should contain 200 microliters of cells in broth. Be sure to include wells containing broth with no cells- for negative control.
-*Be sure to include wells containing broth with no cells- for negative control.
+#Open Gen 5 software by double clicking on the desktop shortcut.
 #Click on Existing protocol OR move to step 3
 #Click on Create New Protocol

@@ Line 1: / Line 1: @@
 Synergy Machine Protocol
 #All Wells should contain 200 microliters of cells in broth.
-**Be sure to include wells containing broth with no cells- for negative control.
+*Be sure to include wells containing broth with no cells- for negative control.
 #Open Gen 5 software
 #Click on Existing protocol OR move to step 3

@@ Line 1: / Line 1: @@
 Synergy Machine Protocol
 #All Wells should contain 200 microliters of cells in broth.
-##Be sure to include wells containing broth with no cells- for negative control.
+**Be sure to include wells containing broth with no cells- for negative control.
 #Open Gen 5 software
 #Click on Existing protocol OR move to step 3

@@ Line 26: / Line 26: @@
 #[[File:CaptureRFP.PNG]]
 ##585 nm/615 nm (100 gain) are the optimal excitation/emission wavelengths for use with RFP, as they resulted in the highest fluorescence readings.
-#[[File:Optimal GFP for ratio.png]]
+#[[File:Fake_GFP.PNG]]
 ##460 nm (excitation) and 490 nm (emission) are the optimal wavelengths to use with GFP and RFP in the same well, in order to minimize noise/interference from RFP at GFP wavelengths.

← Older revision		Revision as of 19:48, 7 June 2012
Line 27:		Line 27:
	##585 nm/615 nm (100 gain) are the optimal excitation/emission wavelengths for use with RFP, as they resulted in the highest fluorescence readings.		##585 nm/615 nm (100 gain) are the optimal excitation/emission wavelengths for use with RFP, as they resulted in the highest fluorescence readings.
	#[[File:Optimal GFP for ratio.png]]		#[[File:Optimal GFP for ratio.png]]
		+	##460 nm (excitation) and 490 nm (emission) are the optimal wavelengths to use with GFP and RFP in the same well, in order to minimize noise/interference from RFP at GFP wavelengths.

← Older revision		Revision as of 19:47, 7 June 2012
Line 26:		Line 26:
	#[[File:CaptureRFP.PNG]]		#[[File:CaptureRFP.PNG]]
	##585 nm/615 nm (100 gain) are the optimal excitation/emission wavelengths for use with RFP, as they resulted in the highest fluorescence readings.		##585 nm/615 nm (100 gain) are the optimal excitation/emission wavelengths for use with RFP, as they resulted in the highest fluorescence readings.
		+	#[[File:Optimal GFP for ratio.png]]