Synergy Machine Protocol for Bio113 - Revision history

Macampbell at 12:27, 20 October 2022

2022-10-20T12:27:45Z

Macampbell at 16:05, 9 August 2022

2022-08-09T16:05:46Z

Macampbell at 20:57, 18 July 2017

2017-07-18T20:57:01Z

Macampbell: Created page with " == Synergy Machine Protocol == # All Wells should contain 200 µL of liquid. Be sure to include wells containing broth with no cells for negative control. # We use the wavel..."

2017-07-18T20:55:50Z

Created page with " == Synergy Machine Protocol == # All Wells should contain 200 µL of liquid. Be sure to include wells containing broth with no cells for negative control. # We use the wavel..."

New page

== Synergy Machine Protocol ==

# All Wells should contain 200 µL of liquid. Be sure to include wells containing broth with no cells for negative control.
# We use the wavelengths of 460nm (excitation) and 490 nm(emission) for GFP, and 585 nm(excitation) and 615 nm (emission) for RFP, to minimize noise/interference. However, some of the GFP reading will be from bleeding over of RFP.
# If you are measuring GFP and RFP, measure GFP before RFP.
# Place the 96-well plate inside the machine, and be sure to put well A1 near the marker for A1.
# After the machine has run, you can export the data to Excel using the Excel button on the banner of the window that pops up.
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Note- if only a few wells give a value of OVRFLW, try lowering the gain from 100 to 95 or 90 (or lower if necessary). Enter the value on the page with the wavelengths for fluorescence.

@@ Line 2: / Line 2: @@
 == Synergy Machine Protocol ==
-# All wells should contain exactly 200 µL of liquid. Be sure to include wells containing broth with no cells for negative control.
+# All wells should contain exactly 200 µL of liquid. Be sure to include wells containing broth with no cells for your blank.
 # We use the wavelengths of 460 nm (excitation) and 490 nm(emission) for GFP, and 585 nm(excitation) and 615 nm (emission) for RFP, to minimize noise/interference. However, some of the GFP reading will be from bleeding over of RFP.
 # If you are measuring GFP and RFP, measure GFP before RFP.

@@ Line 5: / Line 5: @@
 # We use the wavelengths of 460 nm (excitation) and 490 nm(emission) for GFP, and 585 nm(excitation) and 615 nm (emission) for RFP, to minimize noise/interference. However, some of the GFP reading will be from bleeding over of RFP.
 # If you are measuring GFP and RFP, measure GFP before RFP.
 # Choose the protocol called '''Bio113 Synthetic Biology Research'''
 # Place the 96-well plate inside the machine, and be sure to put well A1 near the marker for A1.