Test pLuxR/cI - Revision history

AnGordon at 20:17, 15 July 2008

2008-07-15T20:17:20Z

AnGordon at 19:48, 30 June 2008

2008-06-30T19:48:45Z

New page

luxR/CI hybrid promoter is constitutively off. Ligate with GFP on the 3’ end. If luxR is present first then add AI-1, the bacteria should glow green. Then add CI, the bacteria should not glow.

Some promoter→LuxR+t+t+pLuxR/cI→GFP does not glows

add AI-1

Some promoter→LuxR+t+t+pLuxR/cI→GFP glows

add AI-1

Some promoter→LuxR+cI+t+t+pLuxR/cI→GFP does not glow

{| border="1" cellpadding="2"
!width="30"|LuxR
!width="30"|cI
!width="30"|AI-1 + GFP
!width="30"|AI-1 - GFP
|-
| 0 || 0 || 0 || 0
|-
| 1 || 0 || 1 || 0
|-
| 0 || 1 || 0 || 0
|-
| 1 || 1 || 0 || 0
|}

← Older revision		Revision as of 20:17, 15 July 2008
Line 1:		Line 1:
	luxR/CI hybrid promoter is constitutively off. Ligate with GFP on the 3’ end. If luxR is present first then add AI-1, the bacteria should glow green. Then add CI, the bacteria should not glow.		luxR/CI hybrid promoter is constitutively off. Ligate with GFP on the 3’ end. If luxR is present first then add AI-1, the bacteria should glow green. Then add CI, the bacteria should not glow.

−	Some promoter→LuxR+t+t+pLuxR/cI→GFP does not ~~glows~~	+	Some promoter→LuxR+t+t+pLuxR/cI→GFP does not glow