Test pMnt/Lac - Revision history

AlAllen at 16:58, 16 July 2008

2008-07-16T16:58:12Z

AlAllen at 16:55, 16 July 2008

2008-07-16T16:55:04Z

AlAllen at 16:53, 16 July 2008

2008-07-16T16:53:29Z

AlAllen at 16:38, 8 July 2008

2008-07-08T16:38:40Z

AlAllen at 14:54, 1 July 2008

2008-07-01T14:54:38Z

AlAllen at 14:54, 1 July 2008

2008-07-01T14:54:12Z

AlAllen at 14:53, 1 July 2008

2008-07-01T14:53:49Z

AlAllen at 14:50, 1 July 2008

2008-07-01T14:50:58Z

AlAllen at 14:41, 1 July 2008

2008-07-01T14:41:37Z

AlAllen at 14:40, 1 July 2008

2008-07-01T14:40:57Z

@@ Line 3: / Line 3: @@
 '''To Test repression of promoter'''
-Some promoter→RBS+LacI+t+t+pLac/Mnt→RBS+GFP           ''does not glow''
+Some promoter→RBS+LacI+t+t+pLac/Mnt→RBS+GFP             <nowiki>''does not glow''</nowiki>
 [http://partsregistry.org/Part:BBa_I13453 I13453] → [http://partsregistry.org/Part:BBa_B0034 B0034] +LacI+TT+ [http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]
-Some promoter→RBS+Mnt+t+t+pLac/Mnt→GFP               ''does not glow''
+Some promoter→RBS+Mnt+t+t+pLac/Mnt→GFP                  <nowiki>''does not glow''</nowiki>
 [http://partsregistry.org/Part:BBa_I13453 I13453] → [http://partsregistry.org/Part:BBa_B0034 B0034] +Mnt+TT+ [http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]
-Some promoter→RBS+LacI+RBS+Mnt+t+t+pLac/Mnt→GFP      ''does not glow''
+Some promoter→RBS+LacI+RBS+Mnt+t+t+pLac/Mnt→GFP         <nowiki>''does not glow''</nowiki>
 [http://partsregistry.org/Part:BBa_I13453 I13453] → [http://partsregistry.org/Part:BBa_B0034 B0034] +LacI+RBS+Mnt+TT+ [http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]
@@ Line 21: / Line 21: @@
 add IPTG
-Some promoter→IPTG+RBS+LacI+t+t+pLac/Mnt→GFP              ''glows''
+Some promoter→IPTG+RBS+LacI+t+t+pLac/Mnt→GFP                   <nowiki>''glows''</nowiki>
 [http://partsregistry.org/Part:BBa_I13453 I13453] → IPTG+[http://partsregistry.org/Part:BBa_B0034 B0034]+LacI+TT+[http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]
@@ Line 27: / Line 27: @@
-Some promoter→IPTG+RBS+LacI+Mnt+t+t+pLac/Mnt→GFP         '' glows''
+Some promoter→IPTG+RBS+LacI+Mnt+t+t+pLac/Mnt→GFP               <nowiki>'' glows''</nowiki>
 [http://partsregistry.org/Part:BBa_I13453 I13453] →IPTG+ [http://partsregistry.org/Part:BBa_B0034 B0034] +LacI+RBS+Mnt+TT+ [http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]

@@ Line 3: / Line 3: @@
 '''To Test repression of promoter'''
-Some promoter→RBS+LacI+t+t+pLac/Mnt→RBS+GFP    does not glow
+Some promoter→RBS+LacI+t+t+pLac/Mnt→RBS+GFP           ''does not glow''
 [http://partsregistry.org/Part:BBa_I13453 I13453] → [http://partsregistry.org/Part:BBa_B0034 B0034] +LacI+TT+ [http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]
-Some promoter→RBS+Mnt+t+t+pLac/Mnt→GFP    does not glow
+Some promoter→RBS+Mnt+t+t+pLac/Mnt→GFP               ''does not glow''
 [http://partsregistry.org/Part:BBa_I13453 I13453] → [http://partsregistry.org/Part:BBa_B0034 B0034] +Mnt+TT+ [http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]
-Some promoter→RBS+LacI+RBS+Mnt+t+t+pLac/Mnt→GFP    does not glow
+Some promoter→RBS+LacI+RBS+Mnt+t+t+pLac/Mnt→GFP      ''does not glow''
 [http://partsregistry.org/Part:BBa_I13453 I13453] → [http://partsregistry.org/Part:BBa_B0034 B0034] +LacI+RBS+Mnt+TT+ [http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]
@@ Line 21: / Line 21: @@
 add IPTG
-Some promoter→IPTG+RBS+LacI+t+t+pLac/Mnt→GFP     glows
+Some promoter→IPTG+RBS+LacI+t+t+pLac/Mnt→GFP              ''glows''
 [http://partsregistry.org/Part:BBa_I13453 I13453] → IPTG+[http://partsregistry.org/Part:BBa_B0034 B0034]+LacI+TT+[http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]
@@ Line 27: / Line 27: @@
-Some promoter→IPTG+RBS+LacI+Mnt+t+t+pLac/Mnt→GFP    glows
+Some promoter→IPTG+RBS+LacI+Mnt+t+t+pLac/Mnt→GFP         '' glows''
 [http://partsregistry.org/Part:BBa_I13453 I13453] →IPTG+ [http://partsregistry.org/Part:BBa_B0034 B0034] +LacI+RBS+Mnt+TT+ [http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]

@@ Line 13: / Line 13: @@
-Some promoter→RBS+LacI+Mnt+t+t+pLac/Mnt→GFP    does not glow
+Some promoter→RBS+LacI+RBS+Mnt+t+t+pLac/Mnt→GFP    does not glow
-[http://partsregistry.org/Part:BBa_I13453 I13453] → [http://partsregistry.org/Part:BBa_B0034 B0034] +LacI+Mnt+TT+ [http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]
+[http://partsregistry.org/Part:BBa_I13453 I13453] → [http://partsregistry.org/Part:BBa_B0034 B0034] +LacI+RBS+Mnt+TT+ [http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]
 '''To Test the promoter and addition of IPTG'''
@@ Line 21: / Line 21: @@
 add IPTG
-Some promoter→RBS+IPTG+LacI+t+t+pLac/Mnt→GFP     glows
+Some promoter→IPTG+RBS+LacI+t+t+pLac/Mnt→GFP     glows
-[http://partsregistry.org/Part:BBa_I13453 I13453] → [http://partsregistry.org/Part:BBa_B0034 B0034] +IPTG+LacI+TT+ [http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]
+[http://partsregistry.org/Part:BBa_I13453 I13453] → IPTG+[http://partsregistry.org/Part:BBa_B0034 B0034]+LacI+TT+[http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]

@@ Line 15: / Line 15: @@
 Some promoter→RBS+LacI+Mnt+t+t+pLac/Mnt→GFP    does not glow
-http://partsregistry.org/Part:BBa_I13453 I13453] → [http://partsregistry.org/Part:BBa_B0034 B0034] +LacI+Mnt+TT+ [http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]
+[http://partsregistry.org/Part:BBa_I13453 I13453] → [http://partsregistry.org/Part:BBa_B0034 B0034] +LacI+Mnt+TT+ [http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]
 '''To Test the promoter and addition of IPTG'''

← Older revision		Revision as of 14:54, 1 July 2008
Line 24:		Line 24:

	[http://partsregistry.org/Part:BBa_I13453 I13453] → [http://partsregistry.org/Part:BBa_B0034 B0034] +IPTG+LacI+TT+ [http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]		[http://partsregistry.org/Part:BBa_I13453 I13453] → [http://partsregistry.org/Part:BBa_B0034 B0034] +IPTG+LacI+TT+ [http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]
		+
		+

	{\| border="1" cellpadding="2"		{\| border="1" cellpadding="2"

← Older revision		Revision as of 14:54, 1 July 2008
Line 11:		Line 11:

	[http://partsregistry.org/Part:BBa_I13453 I13453] → [http://partsregistry.org/Part:BBa_B0034 B0034] +Mnt+TT+ [http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]		[http://partsregistry.org/Part:BBa_I13453 I13453] → [http://partsregistry.org/Part:BBa_B0034 B0034] +Mnt+TT+ [http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]
		+

	Some promoter→RBS+LacI+Mnt+t+t+pLac/Mnt→GFP does not glow		Some promoter→RBS+LacI+Mnt+t+t+pLac/Mnt→GFP does not glow

← Older revision		Revision as of 14:53, 1 July 2008
Line 11:		Line 11:

	[http://partsregistry.org/Part:BBa_I13453 I13453] → [http://partsregistry.org/Part:BBa_B0034 B0034] +Mnt+TT+ [http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]		[http://partsregistry.org/Part:BBa_I13453 I13453] → [http://partsregistry.org/Part:BBa_B0034 B0034] +Mnt+TT+ [http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]
		+
		+	Some promoter→RBS+LacI+Mnt+t+t+pLac/Mnt→GFP does not glow
		+
		+	http://partsregistry.org/Part:BBa_I13453 I13453] → [http://partsregistry.org/Part:BBa_B0034 B0034] +LacI+Mnt+TT+ [http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]

	'''To Test the promoter and addition of IPTG'''		'''To Test the promoter and addition of IPTG'''

← Older revision		Revision as of 14:50, 1 July 2008
Line 34:		Line 34:
	\| 1 \|\| 1 \|\| 0 \|\| 0		\| 1 \|\| 1 \|\| 0 \|\| 0
	\|}		\|}
		+
		+
		+	'''Steps to Build Hybrid Promoter'''
		+
		+	6/30/08
		+
		+	Perform a Ligation of Mnt/Lac (K091104) with E0240 (RBS + GFP)
		+
		+	Transform ligation over night
		+
		+	7/1/08
		+
		+	Clones were picked and set over night in LB + AMP

@@ Line 18: / Line 18: @@
 Some promoter→RBS+IPTG+LacI+t+t+pLac/Mnt→GFP     glows
-[http://partsregistry.org/Part:BBa_I13453 I13453] → [http://partsregistry.org/Part:BBa_B0034 B0034] +IPTG+LacI+t+TT+ [http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]
+[http://partsregistry.org/Part:BBa_I13453 I13453] → [http://partsregistry.org/Part:BBa_B0034 B0034] +IPTG+LacI+TT+ [http://partsregistry.org/Part:BBa_K091104 K091104]→[http://partsregistry.org/Part:BBa_E0240 E0240]
 {| border="1" cellpadding="2"