Tuning genetic control through promoter engineering - Revision history

Miwaters at 14:57, 25 September 2007

2007-09-25T14:57:39Z

Miwaters at 14:57, 25 September 2007

2007-09-25T14:57:26Z

Miwaters at 17:33, 24 September 2007

2007-09-24T17:33:10Z

Miwaters at 04:02, 22 September 2007

2007-09-22T04:02:49Z

Miwaters at 04:02, 22 September 2007

2007-09-22T04:02:36Z

Miwaters: /* Online Supplemental Material */

2007-09-22T04:02:18Z

‎Online Supplemental Material

Miwaters: /* Online Supplemental Material */

2007-09-22T04:01:34Z

‎Online Supplemental Material

Miwaters: /* Online Supplemental Material */

2007-09-22T04:00:17Z

‎Online Supplemental Material

Miwaters: /* Online Supplemental Material */

2007-09-22T03:59:12Z

‎Online Supplemental Material

Miwaters at 03:58, 22 September 2007

2007-09-22T03:58:30Z

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 In molecular biology, reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique for amplifying a defined piece of a ribonucleic acid (RNA) molecule. The RNA strand is first reverse transcribed into its DNA complement or complementary DNA, followed by amplification of the resulting DNA using polymerase chain reaction. (from Wikipedia)
-[http://www.bio.davidson.edu/Courses/immunology/Flash/RT_PCR.html Davidson RT-PCR Animation]<br> [http://en.wikipedia.org/wiki/TaqMan wikipedai quantitative PCR]
+[http://www.bio.davidson.edu/Courses/immunology/Flash/RT_PCR.html Davidson RT-PCR Animation]<br> [http://en.wikipedia.org/wiki/TaqMan Wikipedai quantitative PCR]
 ==Application of Promoter Library==

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 In molecular biology, reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique for amplifying a defined piece of a ribonucleic acid (RNA) molecule. The RNA strand is first reverse transcribed into its DNA complement or complementary DNA, followed by amplification of the resulting DNA using polymerase chain reaction. (from Wikipedia)
-[http://www.bio.davidson.edu/Courses/immunology/Flash/RT_PCR.html Davidson RT-PCR Animation]
+[http://www.bio.davidson.edu/Courses/immunology/Flash/RT_PCR.html Davidson RT-PCR Animation]<br> [http://en.wikipedia.org/wiki/TaqMan wikipedai quantitative PCR]
 ==Application of Promoter Library==

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 Flow Cytometry is a technique for counting, examining, and sorting microscopic particles suspended in a stream of fluid. It allows simultaneous multiparametric analysis of the physical and/or chemical characteristics of single cells flowing through an optical and/or electronic detection apparatus. (from Wikipedia)
 [http://www.bio.umass.edu/mcbfacs/intro.htm Helpful Flow Cytometry Link]
 '''RT-PCR''' <br>

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 ==Online Supplemental Material==
-[http://www.bio.davidson.edu/Courses/Synthetic/papers/MWPrimers.pdf Primers]
+[http://www.bio.davidson.edu/Courses/Synthetic/papers/MWPrimers.pdf Primers]<br>
 [http://http://www.bio.davidson.edu/Courses/Synthetic/papers/MWFig5.pdf Supporting Figure 5]
 Fig. 5. The functional promoter library was analyzed by using flow cytometry. The relative average geometric mean fluorescence of the members of the library is illustrated above and exhibited a nearly 3-log fold range after 14 h in a minimal media with 0.1% casamino acids.<br>

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 [http://www.bio.davidson.edu/Courses/Synthetic/papers/MWPrimers.pdf Primers]
 [http://http://www.bio.davidson.edu/Courses/Synthetic/papers/MWFig5.pdf Supporting Figure 5]
 Fig. 5. The functional promoter library was analyzed by using flow cytometry. The relative average geometric mean fluorescence of the members of the library is illustrated above and exhibited a nearly 3-log fold range after 14 h in a minimal media with 0.1% casamino acids.<br>

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 [http://http://www.bio.davidson.edu/Courses/Synthetic/papers/MWFig5.pdf Supporting Figure 5]
-Fig. 5. The functional promoter library was analyzed by using flow cytometry. The relative average geometric mean fluorescence of the members of the library is illustrated above and exhibited a nearly 3-log fold range after 14 h in a minimal media with 0.1% casamino acids.
+Fig. 5. The functional promoter library was analyzed by using flow cytometry. The relative average geometric mean fluorescence of the members of the library is illustrated above and exhibited a nearly 3-log fold range after 14 h in a minimal media with 0.1% casamino acids.<br>
 [http://www.bio.davidson.edu/Courses/Synthetic/papers/MWFig6.pdf Supporting Figure 6]
-Fig. 6. Above are representative light-field (right) and false-color dark-field (Left) photomicrographs of clones with highly heterogeneous expression of GFP. These promoters were not further analyzed and are not included in the functional promoter library.
+Fig. 6. Above are representative light-field (right) and false-color dark-field (Left) photomicrographs of clones with highly heterogeneous expression of GFP. These promoters were not further analyzed and are not included in the functional promoter library.<br>
-Supporting Figure 7
+[http://www.bio.davidson.edu/Courses/Synthetic/papers/MWFig7.pdf Supporting Figure 7]
 Fig. 7. In contrast, representative clones chosen for further analysis had much more homogenous distributions of fluorescence. Below, two representative sets of photomicrographs are shown. The top two images correspond to a promoter with a relative promoter strength metric of 0.124 and the bottom two to 0.417.

← Older revision		Revision as of 04:00, 22 September 2007
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	[http://www.bio.davidson.edu/Courses/Synthetic/papers/MWPrimers.pdf Primers]		[http://www.bio.davidson.edu/Courses/Synthetic/papers/MWPrimers.pdf Primers]
		+
		+
		+	Supporting Figure 5
		+	Fig. 5. The functional promoter library was analyzed by using flow cytometry. The relative average geometric mean fluorescence of the members of the library is illustrated above and exhibited a nearly 3-log fold range after 14 h in a minimal media with 0.1% casamino acids.
		+	Supporting Figure 6
		+	Fig. 6. Above are representative light-field (right) and false-color dark-field (Left) photomicrographs of clones with highly heterogeneous expression of GFP. These promoters were not further analyzed and are not included in the functional promoter library.
		+	Supporting Figure 7
		+	Fig. 7. In contrast, representative clones chosen for further analysis had much more homogenous distributions of fluorescence. Below, two representative sets of photomicrographs are shown. The top two images correspond to a promoter with a relative promoter strength metric of 0.124 and the bottom two to 0.417.

← Older revision		Revision as of 03:59, 22 September 2007
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	==Online Supplemental Material==		==Online Supplemental Material==
		+
		+	[http://www.bio.davidson.edu/Courses/Synthetic/papers/MWPrimers.pdf Primers]

← Older revision		Revision as of 03:58, 22 September 2007
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	[[Promoter Strength Metric]]		[[Promoter Strength Metric]]
		+
		+	==Online Supplemental Material==