Cawillis at 17:00, 9 September 2018

2018-09-09T17:00:33Z

Cawillis at 17:00, 9 September 2018

2018-09-09T17:00:11Z

Cawillis: Created page with "Optimal Restriction Enzymes at 37 degrees C: '''3’ Overhang:''' 2 Sticky Ends- • AsiSI * • PacI • PvuI-HF * • SacII * 4 Sticky Ends- • AatII * • BmtI/BmtI-HF..."

2018-09-09T16:59:06Z

Created page with "Optimal Restriction Enzymes at 37 degrees C: '''3’ Overhang:''' 2 Sticky Ends- • AsiSI * • PacI • PvuI-HF * • SacII * 4 Sticky Ends- • AatII * • BmtI/BmtI-HF..."

New page

Optimal Restriction Enzymes at 37 degrees C:

'''3’ Overhang:'''
2 Sticky Ends-
• AsiSI *
• PacI
• PvuI-HF *
• SacII *
4 Sticky Ends-
• AatII *
• BmtI/BmtI-HF
• FseI *
• KpnI-HF
• NsiI-HF
• PstI-HF
• SacI-HF *
• SbfI/SbfI-HF
• SphI-HF

'''5’ Overhang:'''
2 Sticky Ends-
• AcII: *
• ClaI *
• NarI *
• NdeI
4 Sticky Ends-
• AflII
• AgeI-HF
• ApaLI *
• AscI *
• AvrII
• BamHI-HF
• BclI-HF *
• BsaI-HF V2 *
• BspHI *
• BsrGI-HF
• EagI-HF *
• EcoRI-HF *
• HindIII-HF
• KasI *
• MfeI/MfeI-HF
• MluI-HF *
• NcoI-HF
• NgoMIV *
• NheI-HF 4SE *
• NotI-HF *
• PspOMI *
• SalI-HF *
• SpeI/SpeI-HF
• XbaI *
• XhoI *
• XmaI *

'''Blunt cutters:'''
• AfeI *
• BsrBI*
• DraI
• EcoRV *
• FspI *
• HpaI *
• MscI *
• NaeI *
• NruI-HF *
• PmeI *
• PmlI *
• PvuII-HF
• ScaI-HF
• SfoI *
• SnaBI *
• SspI-HF
• StuI *

← Older revision		Revision as of 17:00, 9 September 2018
Line 71:		Line 71:
	• StuI *		• StuI *

−	* - is affected by methylation (CpG, dam, dcm)	+	* - is affected by methylation (CpG, dam, dcm)

← Older revision		Revision as of 17:00, 9 September 2018
Line 70:		Line 70:
	• SspI-HF		• SspI-HF
	• StuI *		• StuI *
		+
		+	* - is affected by methylation (CpG, dam, dcm)

User:Cawillis - Revision history

Cawillis at 17:00, 9 September 2018

Cawillis at 17:00, 9 September 2018

Cawillis: Created page with "Optimal Restriction Enzymes at 37 degrees C: '''3’ Overhang:''' 2 Sticky Ends- • AsiSI * • PacI • PvuI-HF * • SacII * 4 Sticky Ends- • AatII * • BmtI/BmtI-HF..."