Using Apes (A Plasmid Editor) - Revision history

Macampbell: /* Download ApE (a Plasmid editor) */

2012-08-08T13:51:53Z

‎Download ApE (a Plasmid editor)

Macampbell: /* Download aPe (a Plasmid editor) */

2012-08-08T13:51:24Z

‎Download aPe (a Plasmid editor)

Macampbell: /* Add Features */

2010-06-09T12:07:52Z

‎Add Features

Macampbell at 13:49, 8 June 2010

2010-06-08T13:49:18Z

Olhoshing at 21:38, 18 August 2009

2009-08-18T21:38:11Z

Letaylor: /* Quickly View Features of Sequence */

2009-07-17T19:21:31Z

‎Quickly View Features of Sequence

Letaylor at 19:09, 17 July 2009

2009-07-17T19:09:22Z

New page

==Quickly View Features of Sequence==
1. Open the .seq file 
2. Download this features file 
3. In the features menu on the toolbar select "Open Feature Library" 
4. Select the file you downloaded in step 3 
5. In the features menu on the toolbar select "Annotate Features Using Library" OR press ⌘K 

==Add Features==
1. In the features menu on the toolbar select "Edit Feature Library" 
2. Click on the "New" button 
3. In the top field type the label of the feature you are adding 
In the bottom field type the sequence of the feature you are adding 
4. Highlight the sequence and click the "Uppercase" button 
5. Choose the appropriate feature type and the appropriate color label 
***DO NOT FORGET TO SAVE CHANGES If you wish to add these features to the standard features file, be sure to update the file this page links to.

@@ Line 1: / Line 1: @@
-==Download ApE (a Plasmid editor)==
+==Download ApE (A plasmid Editor)==
 # Go to the web site to [http://www.biology.utah.edu/jorgensen/wayned/ape/ download the latest Windows or Mac version].
 # You will want to use a Feature search or align two sequences to verify your sequencing results.

@@ Line 1: / Line 1: @@
-==Download aPe (a Plasmid editor)==
+==Download ApE (a Plasmid editor)==
 # Go to the web site to [http://www.biology.utah.edu/jorgensen/wayned/ape/ download the latest Windows or Mac version].
 # You will want to use a Feature search or align two sequences to verify your sequencing results.
@@ Line 6: / Line 6: @@
 # It is helpful to identify the BioBrick ends first, so you can see where you are.
 # Some times the sequencing did not work for technical reasons.
 # You can confirm any Ns by looking at the chromatogram.
 ==Quickly View Features of Sequence==

@@ Line 22: / Line 22: @@
 . Highlight the sequence and click the "Uppercase" button<br>
 . Choose the appropriate feature type and the appropriate color label<br>
-'''DO NOT FORGET TO SAVE CHANGES''' If you wish to add these features to the standard features file, be sure to update the file this page links to.
+'''DO NOT FORGET TO SAVE CHANGES''' If you wish to add these features to the standard features file, be sure to update the file this page links to.<br>

← Older revision		Revision as of 13:49, 8 June 2010
Line 1:		Line 1:
		+	==Download aPe (a Plasmid editor)==
		+	# Go to the web site to [http://www.biology.utah.edu/jorgensen/wayned/ape/ download the latest Windows or Mac version].
		+	# You will want to use a Feature search or align two sequences to verify your sequencing results.
		+	# Open the .seq file from your sequence service provider.
		+	# Delete any N's from the first and last parts, but leave "internal" Ns in place.
		+	# It is helpful to identify the BioBrick ends first, so you can see where you are.
		+	# Some times the sequencing did not work for technical reasons.
		+	# You can confirm any Ns by looking at the chromatogram.
		+
	==Quickly View Features of Sequence==		==Quickly View Features of Sequence==
	1. Open the .seq file<br>		1. Open the .seq file<br>

@@ Line 1: / Line 1: @@
 ==Quickly View Features of Sequence==
 . Open the .seq file<br>
-. Download [http://www.bio.davidson.edu/projects/GCAT/Synthetic/iGEMfeatures this] file, called iGEMfeatures<br>
+. Download [http://www.bio.davidson.edu/projects/GCAT/Synthetic/iGEMfeatures this file], called iGEMfeatures<br>
 . In the features menu on the toolbar select "Open Feature Library"<br>
 . Select the file you downloaded in step 3<br>
@@ Line 10: / Line 10: @@
 . Click on the "New" button<br>
 . In the top field type the label of the feature you are adding<br>
-In the bottom field type the sequence of the feature you are adding<br>
+. In the bottom field type the sequence of the feature you are adding<br>
 . Highlight the sequence and click the "Uppercase" button<br>
 . Choose the appropriate feature type and the appropriate color label<br>
-***DO NOT FORGET TO SAVE CHANGES If you wish to add these features to the standard features file, be sure to update the file this page links to.
+'''DO NOT FORGET TO SAVE CHANGES''' If you wish to add these features to the standard features file, be sure to update the file this page links to.