Difference between revisions of "Aspartate Carbamoyltransferase"

Revision as of 03:52, 6 October 2009

I did a blastp search using our species' ACTase protein sequence, the following where the results. . .

Halophilic Species with Protein Similarity

Haloarcula marismortui protein sequence:

  >Haloarcula marismortui ATCC 43049
MRHDHIISAKQLSRGDIETVLDHAADIAADPGAFADRHSDTLLGLLFFEPSTRTKMSFTTAMKRLGGDIV
DMGSVESSSVKKGESLADTVRVVEGYTDALVLRHPMEGSAKMASEFVDVPLVNAGDGAGQHPTQTLLDLY
TIRENAGFDDLTIGIMGDLKYGRTVHSLAHALTTVDASQHFISPESLQLPRSVRYDLHEAGAGIREHTEL
DDILPELDVLYVTRIQAERFPDESEYREVAGQYQIDGDTLAAAKDDLTVMHPLPRVDEIAHDVDETTHAQ
YFQQAHNGVPVRMALLDLMLGGDQ

Halorhabdus utahensis protein sequence:

>gi|257051607|ref|YP_003129440.1| aspartate carbamoyltransferase [Halorhabdus utahensis DSM 12940]
MRHDHIISAKQLSRSDIEEVLDRAAEIARDPTAFRQRHPGALLGLLFFEPSTRTKMSFSASIKRLGGDIV
DMGPVEYSSVKKGESLADTVRVVEGYADALVLRHPMEGSAKMASEFVDVPLINGGDGAGQHPTQTLLDLY
TIRENAGLDDLSIGIMGDLKYGRTVHSLAHALTNFDASQHFISPESLRLPRNVRYDLHEEGATIREHTAL
DTVLDSLDVLYVTRIQEERFPEESEYRKVAGEYRIDAETLESTRDDLTVLHPLPRVDEIDHDVDATSHAQ
YFQQAHNGVPVRMALLDLVLGGESA

Natronomonas pharaonis

>gi|76802397|ref|YP_327405.1| aspartate carbamoyltransferase catalytic subunit [Natronomonas pharaonis DSM 2160]
MRHDHLLSAKQLSRGDIEGVLDRAAEIAADPDAVAEKHSDKLLGLLFFEPSTRTKMSFDTAMKRLGGDTV
DMGSVDSSSVKKGESLADTVRVIEGYTDAIVLRHPSEGSPKLASEFVDVPVINAGDGAGQHPTQTLLDMY
TIRENAGLDDLTIGIMGDLKYGRTVHSLAHALTNFDVSQHFISPESLQLPRSVRYDLHESGAQVREHTDI
ESVLETLDVLYVTRIQRERFPDENEYRQVAGEYRIDADLLESAKDDLTVMHPLPRVSEIAADVDETEHAT
YFKQAHNGVPVRMALLDEML

Halorubrum lacusprofundi

>gi|222479710|ref|YP_002565947.1| aspartate carbamoyltransferase [Halorubrum lacusprofundi ATCC 49239]
MRQDHLITATQLSRDDIEAVLDRAREVADDPAAYADRHAGSVLALCFFEPSTRTRMSFDSAAKRLGMNTI
GMGDVDSSSVSKGESLSDTVRVIEGYADALVLRHPSEGAATLAAERVSVPVVNAGDGAGQHPSQTLLDLH
TIRENHGLDDLTVGIMGDLKYGRTVHSLAAALTEFDANQHFISPESLRLPRSVRFDLHETGAQVREHTDL
EGVLGELDVLYVTRIQKERFPDENEYHRVAGEYQIDAETLEDAPDDLTVMHPLPRVDEIAPDVDETDHAT
YFEQAHNGIPVRMALLDILLENAEGDEGMEVDR

Halobacterium salinarum

>gi|169237479|ref|YP_001690683.1| aspartate carbamoyltransferase catalytic subunit [Halobacterium salinarum R1]
MDLDHIITSKQLSRADIETVLDRAAEIDADPSAFADRYENTVLGLLFFEPSTRTKMSFDTAMKRLGGDIV
DMGSVDSSSVKKGETLADTVRVIEGYADALVLRHPNQGAAQMASEFVDVPLLNAGDGAGHHPTQTLLDLY
TIRENAGLEDLTIGIMGDLKYGRTVHSLSRALTNFDVDQHFISPESLQLPRDVVYDLEQESGTRVREHDS
FEDVLPELDILYVTRIQRERFPDENEYQKVAGEYQLTATHLEAAKDDLTIMHPLPRVDEIAPEIDDTDHA
AYFEQAHNGVPVRMALLDLVLSADGGVDDE

Halogeometricum borinquense

>gi|227879444|ref|ZP_03997306.1| aspartate carbamoyltransferase [Halogeometricum borinquense DSM 11551]
MRQDHLISATQLSRDDVEAVLDRAADIDADPAAWRQRHAGTVLGLCFFEPSTRTRMSFDTAMKRLGGRTV
DMGPVESSSVKKGESLADTVRVVEGYGDAIVLRHPLEGAAKMASEFVDVPVINAGDGAGQHPTQTLLDLY
TIRENAGLDDLTIGIMGDLKYGRTVHSLAEALTNFDARQHFISPESLRLPRNVRYDLHGSGAQVREHTEL
SEVLPELDVLYVTRIQRERFPDENEYRKVAGQYQIDADDLSDAKDSLTVMHPLPRVDEIAPEVDETAHAK
YFEQAHNAVPVRMALLDMLLEDN

Natrialba magadii

>gi|224822047|ref|ZP_03695167.1| aspartate carbamoyltransferase [Natrialba magadii ATCC 43099]
MRHDHLITSKQLSRADIETVLDRAAEIADDPDAVANRHANAVLGLLFFEPSTRTKMSFETAMKRLGGSVV
DMGSVESSSVKKGETLADTVRVIEGYADSLVLRHPKQGAAAMACEFVDVPLLNAGDGAGHHPTQTMLDLY
TIRENAGLDDLTIGIMGDLKYGRTVHSLSYALTNFDTHQHFISPESLQLPREVVYDLHQQDDTQIREHEA
LESVLPSLDVLYVTRIQRERFPDENEYQKVAGEYQIDLETLEDASDDLTIMHPLPRVDEIAPEIDETEHA
AYFEQAHNGVPVRMALLDLLLSGGETDE

Haloquadratum walsbyi

>gi|110667833|ref|YP_657644.1| aspartate carbamoyltransferase catalytic subunit [Haloquadratum walsbyi DSM 16790]
MHQDHLLSTTQLSREDIETILDRAAAIEMNPDGWEAQYSGTVLGLCFFEPSTRTRMSFDAAMKRLGGRTI
DMGSVDNSSISKGETLADTVRVIAGYADAIVLRHPSEGAAKLAAEFVDIPVINAGDGAGQHPTQTLLDLY
TMRENVGLDEITVGIAGDLKYGRTVHSLAAALSNFDVRQHFISPESLQLPRNIRYDLHDSGSQVREHTDI
ESILPELDVLYVTRIQRERFPDENEYQRVAGQYRVDTDILSHADNNLTVMHPLPRVDEIAPEVDTTEHAA
YFEQAHNGVPVRMALLDALIGRAK

Marine Bacterium

Vibrio parahaemolyticus

>gi|153839282|ref|ZP_01991949.1| aspartate carbamoyltransferase [Vibrio parahaemolyticus AQ3810]
MSKIPQRSILEIVITVIGKKIMANSLYQKHIISIPELSREELELIVETAGNLKKEPRPELIKNKVVASCF
FEPSTRTRLSFETAIQRIGGDVIGFDNDGNTSLAKKGETLSDSVQVISSYVDAFVMRHPQEGAARLASEF
SNGVPVINAGDGANQHPTQTLLDLYTIAETQGRLDNLNVAFVGDLKYGRTVHSLTQALAKFNNIRFYFVA
PDALAMPEYICEELDEAGIEYSLHTEMEAVIPELDILYMTRVQKERFDESEYAHIKSAYILTAALLKGAR
ENLKVLHPLPRVDEITTDVDKTPHAYYFQQAQNGVYARQALLALVLNETL

Vibrio harveyi

>gi|153834576|ref|ZP_01987243.1| aspartate carbamoyltransferase [Vibrio harveyi HY01]
MANSLYQKHIISIPELSREELELIVETAGQLKKEPRPELIKNKVVASCFFEPSTRTRLSFETAIQRIGGD
VIGFDNGGNTSLAKKGETLSDSVQVISSYVDAFVMRHPQEGAARLASEFSNGVPVINAGDGANQHPTQTL
LDLYTIAETQGRLDNLNVAFVGDLKYGRTVHSLTQALAKFNNIRFFFVAPEALAMPDYICEELDEAGIQY
SLHTDMESVIPELDILYMTRVQKERFDESEYAHIKSAYILTAALLEGARENLKVLHPLPRVDEITTDVDV
TPHAYYFQQAENGVYARQALLALVLNETL

Vibrionales bacterium

>gi|148977756|ref|ZP_01814312.1| aspartate carbamoyltransferase catalytic subunit [Vibrionales bacterium SWAT-3]
MANSLYQKHIISIPELSREELELIVQTAGQLKAEPNPELIKNKVVASCFFEPSTRTRLSFETAIQRIGGD
VIGFDSGGNTSLAKKGETLADSVQVISSYVDAYVMRHPQEGAARLASEFSNGVPVINAGDGANQHPTQTL
LDLFSIAETQGRLDNLNVAFVGDLKYGRTVHSLTQALAKFDNICFYFVAPEALAMPDYICEELDEAGIKY
QLLTDMEDVIPELDVLYMTRVQKERFDESEYAHIKSAYILTAALLENARDNLKVLHPLPRVDEITVDVDK
TPYAYYFQQAENGVYAREALLALVLNETL

Vibrio splendidus

>gi|218710660|ref|YP_002418281.1| aspartate carbamoyltransferase catalytic subunit [Vibrio splendidus LGP32]
MANSLYQKHIISIPELSREELELIVQTAGQLKAEPNPELIKNKVVASCFFEPSTRTRLSFETAIQRIGGD
VIGFDSGGNTSLAKKGETLADSVQVISSYVDAYVMRHPQEGAARLASEFSNGVPVINAGDGANQHPTQTL
LDLFSIAETQGRLDNLNVAFVGDLKYGRTVHSLTQALAKFDNICFYFVAPEALAMPDYICEELDEAGIKY
QLLTDMEDVIPELDVLYMTRVQKERFDESEYAHIKSAYILTASMLENARDNLKVLHPLPRVDEITVDVDK
TPYAYYFQQAENGVYAREALLALVLNETL

Vibrio vulnificus

>gi|27364849|ref|NP_760377.1| aspartate carbamoyltransferase catalytic subunit [Vibrio vulnificus CMCP6]
MTNSLYKKHIISIPELSRAELELIVKTAGQLKAEPNPELIKNKVVASCFFEPSTRTRLSFETAIQRIGGD
VIGFDDGGNTSLAKKGETLSDSVQVISNYVDAFVMRHPQEGAARLASEFSNGVPVINAGDGANQHPTQTL
LDLFTISETQGRLDNLNVAFVGDLKYGRTVHSLTQALAKFNNIRFFFVAPDALAMPDYILEDLDEAGISY
SLHTDMETVIPELDILYMTRVQKERFDESEYAHIKSAYILTAALLEGARENLKVLHPLPRVDEITTDVDK
TPHAYYFQQAGNGVYAREALLALVLNESL

Non-salt loving organisms with top hits

Methanosaeta thermophila- thermophilic archaea

>gi|116754557|ref|YP_843675.1| aspartate carbamoyltransferase catalytic subunit [Methanosaeta thermophila PT]
MLKRRHVLSMKDFSREEIDEILETAESLEPYARGKGSDILEGKILALMFFEPSTRTRMSFETAMKRLGGK
TINLGSAEASSIAKGESLADTIRVVGGYADAIVIRHPKEGSARLAAEFSPVPVLNAGDGAGHHPTQTLLD
LYTIKKESHLDDLSIALVGDLKYGRTVHSLAYALSLYGADIYLVSPPTLRMPEQIIGDLSRMGTRVREVS
DLREVIKEVDVLYITRIQRERFPDPVEYNRVARSYSITIRDLEGVKPELRIMHPLPRVDEISPSVDETEH
AVYFRQSFYGVPVRMALLKMILED

Archaeoglobus fulgidus- sulfur reducing archaea

>gi|11497726|ref|NP_068947.1| aspartate carbamoyltransferase, catalytic subunit (pyrB) [Archaeoglobus fulgidus DSM 4304]
MMRHLISIGDLDREDINYILKKAEEFEDVARGEKKLRILEGKILGNLFFEPSTRTRMSFETAMKRLGGDV
INMTAQEASSIAKGETLADTIRVVSGYCDAIVIRHPLEGAARFAAENSSVPVINAGDGAGQHPTQTLLDL
YTIKKECGRLDGITIALMGDLKYSRTIHSLIKALALFDMRIYLISPEALALPEDIIEDVSAEIRRARLEE
VISEIDVLYVTRIQKERFPDEEEYRKVSGSYRITAETLKSAKDSMIVMHPLPRVDEIHPSVDSTKHARYF
QQSFYGVPVRMAILSEVML

Methanocaldococcus fervens- autotrophic hyperthermophilic archaea

>gi|256810708|ref|YP_003128077.1| aspartate carbamoyltransferase [Methanocaldococcus fervens AG86]
MRHLISMKDIGKEEILEILEEAKKMEELLNTKRPLKLLEGKILATVFYEPSTRTRLSFETAMKRLGGEVI
TMTDLKSSSVAKGESLIDTIRVISGYADIIVLRHPSEGAARLASEYSQVPIINAGDGSNQHPTQTLLDLY
TIMREIGKIDGIKITFVGDLKYGRTVHSLVYALSLFNDVEMYFISPKELKMPRDIIEDLKSKNIKFYEKE
SLDEIGDDIDVLYVTRIQKERFPDPNEYEKVKGSYKIKKEYVEGKKFIIMHPLPRVDEIDYEVDNLPQAK
YFKQSFYGIPVRMAILKKLIEDNE

Chromobacterium violaceum - bacterium

>gi|34495824|ref|NP_900039.1| aspartate carbamoyltransferase catalytic subunit [Chromobacterium violaceum ATCC 12472]
MYRKHIISIPDFSREELELVVDTAARLKQSPRGDLLQDKLVASCFFEPSTRTRLSFETAVQRLGGNIIGF
ADGGNTSAKKGETLADSIKIISSYTDAVVMRHPKEGAARLASEFSAVPVINGGDGSNQHPTQTLLDLFSI
RETQGRLDGLTVAFAGDLKYGRTVHSLAQALSLFGARFYFVSPEVLAMPDYICEELDEKGISYTVADSLE
AVIPEVDVLYMTRVQRERFDEAEFRKIQGQYALRADMLKHARPGMKVLHPLPRVDEIAVDVDATPHAYYF
EQAKNGVYARQALLSLVLNETV

Vibrio cholerae- bacterium causes cholera

>gi|229527555|ref|ZP_04416947.1| aspartate carbamoyltransferase [Vibrio cholerae 12129(1)]
MLKPPLWGAFLWLLENIGKTIMANSLYQKHIISIAELSRAELELIVKTAGQLKAQPNPELIKHKVVASCF
FEPSTRTRLSFETAIQRIGGSVIGFDNGGNTSLAKKGETLADSVRVIASYVDAFVMRHPQEGAARLASEF
SNGTPVINAGDGSNQHPSQTLLDLYSIYETQGRLDNLDVAFVGDLKYGRTVHSLAQALAKFDNNRFYFVA
PEALAMPDYICEELDEAGVKYQVFSDMESVIPELDILYMTRVQKERFDESEYAHIKSAYILTAAHLSEAR
SNLKVLHPLPRVDEITTDVDKTPHAYYFEQVENGVYAREALLALVLNESL

Aeromonas hydrophila- bacterium mainly found in warm areas

>gi|117619960|ref|YP_858511.1| aspartate carbamoyltransferase catalytic subunit [Aeromonas hydrophila subsp. hydrophila ATCC 7966]
MQTMTNPLYKKHVISISDLTRPDMELVVATAQRLKAEPDTRLLKDKLVASCFFEASTRTRLSFETAVQRL
GGNIIGFADGGNTSAKKGETLADSIKIIGSYTDAVVMRHPKEGAARLASEFSRVPVINGGDGSNQHPTQT
LLDLFSIHETQGKLDGLNVAFVGDLKYGRTVHSLAQALSLFNCRFFFISPEALAMPDYICEELEEKGIQF
SVHETMEEVMPELDILYMTRVQKERFDETEYKHMAAKFVLEVATLEGAKPTMKILHPLPRVDEIDVAVDK
TPHAYYFQQAENGVYARQALLALVLNETV

Picrophilus torridus- thermoacidiphil archaea

>gi|48478037|ref|YP_023743.1| aspartate carbamoyltransferase catalytic subunit [Picrophilus torridus DSM 9790]
MLKNNSVISINDLSDEDLNLIFNTADSMAGNLKNGSQIKTMSGRIMATLFFEPSTRTRLSFESAMQRLGG
SVISMADSKSSSTAKGETLADTTRMVSSYSDIIVVRHPLEGAARLVQKFSSRPVINAGDGSGEHPTQTLV
DLYTIKKSFGDINNLEISIIGDLRYGRTVHSLLLALSRYNVRINLVSPDLLRLPGHVMSRLKNIKINEYN
DLNNVIESSDVFYVTRIQRERFTDKNDYNKVIGTYGITEKDTEKMKENAIIMHPLPRVDEISSSVDNTKN
AKYFIQAANGIPVRMALISLILGD

Tolumonas auensis

>gi|237809542|ref|YP_002893982.1| aspartate carbamoyltransferase [Tolumonas auensis DSM 9187]
MANPLYKKNILSIADLSRTDLELVVQTASQLKQSPRHDLLQHKVIASCFFEASTRTRLSFETAVHRLGGS
VIGFADAGNTSLAKKGETLADSVRVITSYTDAFVIRHPQEGAARLSAEFSSVPVINAGDGSNQHPTQTLL
DLFSIYETQGTLDGLKLAFVGDLKYGRTVHSLAQALSLFGARFYFISPEALAMPDYICEELTEKGIEFSF
HDTIEEVMPELDILYMTRVQKERFEESEYRHIAAKFVLSADELKTAKPNMKILHPLPRVDEIHTDVDDTN
HAYYFQQAGNGVYARQALLALVLNEEV

Thermoplasma acidophilum- archaea

>gi|16081678|ref|NP_394049.1| aspartate carbamoyltransferase catalytic subunit [Thermoplasma acidophilum DSM 1728]
MLKNRSVVSIEDVDIDDLNDLFDLSDSMLKTIEKGGSTDLLRNRIMATLFYEPSTRTRLSFESAMHRLGG
SVITVSDVKTSSVAKGETLADTIRMASSYSDIIVIRHPLEGAARLASKFANKPVINAGDGSGQHPTQTIL
DLYTIKRETGSIDGKTITMVGDLRYGRTIHSLIIALSRFDVRINLVSPQILKLPEYVLTKIGDRSRIMEY
DDLSKVIEDTDVLYVTRIQKERFSDQNEYQSVIGSYSVDRDLVSRMKKDAIIMHPLPRIDEIKPEVDELP
QARYFKQAYYGVPVRMALIYRILGD